Glyphosate resistance in perennial Sorghum halepense (Johnsongrass), endowed by reduced glyphosate translocation and leaf uptake

Mostrar todas las versiones(2)

Background: In a large cropping area of northern Argentina, Sorghum halepense (Johnsongrass) has evolved towards glyphosate resistance. This study aimed to determine the molecular and biochemical basis conferring glyphosate resistance in this species. Experiments were conducted to assess target EPSP...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Vila-Aiub, M.M., Balbi, M.C., Distéfano, A.J., Fernández, L., Hopp, E., Yu, Q., Powles, S.B.
Formato: JOUR
Materias:
Glyphosate leaf uptake
Glyphosate translocation
Non-target-site resistance mechanism
Perennial Johnsongrass weed
drug derivative
glycine
glyphosate
herbicide
vegetable protein
assessment method
biochemical composition
biological uptake
carbon isotope
molecular analysis
pesticide resistance
sorghum
translocation
article
drug effect
genetics
herbicide resistance
metabolism
plant leaf
transport at the cellular level
Biological Transport
Glycine
Herbicide Resistance
Herbicides
Plant Leaves
Plant Proteins
Sorghum
Argentina
Sorghum halepense
Sorghum x almum
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_1526498X_v68_n3_p430_VilaAiub
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Background: In a large cropping area of northern Argentina, Sorghum halepense (Johnsongrass) has evolved towards glyphosate resistance. This study aimed to determine the molecular and biochemical basis conferring glyphosate resistance in this species. Experiments were conducted to assess target EPSPS gene sequences and 14C-glyphosate leaf absorption and translocation to meristematic tissues. Results: Individuals of all resistant (R) accessions exhibited significantly less glyphosate translocation to root (11% versus 29%) and stem (9% versus 26%) meristems when compared with susceptible (S) plants. A notably higher proportion of the applied glyphosate remained in the treated leaves of R plants (63%) than in the treated leaves of S plants (27%). In addition, individuals of S. halepense accession R 2 consistently showed lower glyphosate absorption rates in both adaxial (10-20%) and abaxial (20-25%) leaf surfaces compared with S plants. No glyphosate resistance endowing mutations in the EPSPS gene at Pro-101-106 residues were found in any of the evaluated R accessions. Conclusion: The results of the present investigation indicate that reduced glyphosate translocation to meristems is the primary mechanism endowing glyphosate resistance in S. halepense from cropping fields in Argentina. To a lesser extent, reduced glyphosate leaf uptake has also been shown to be involved in glyphosate-resistant S. halepense. © 2011 Society of Chemical Industry.

Ejemplares similares