Glyphosate resistance in perennial Sorghum halepense (Johnsongrass), endowed by reduced glyphosate translocation and leaf uptake

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Background: In a large cropping area of northern Argentina, Sorghum halepense (Johnsongrass) has evolved towards glyphosate resistance. This study aimed to determine the molecular and biochemical basis conferring glyphosate resistance in this species. Experiments were conducted to assess target EPSP...

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Autores principales:	Vila-Aiub, M.M., Balbi, M.C., Distéfano, A.J., Fernández, L., Hopp, E., Yu, Q., Powles, S.B.
Formato:	JOUR
Materias:	Glyphosate leaf uptake Glyphosate translocation Non-target-site resistance mechanism Perennial Johnsongrass weed drug derivative glycine glyphosate herbicide vegetable protein assessment method biochemical composition biological uptake carbon isotope molecular analysis pesticide resistance sorghum translocation article drug effect genetics herbicide resistance metabolism plant leaf transport at the cellular level Biological Transport Glycine Herbicide Resistance Herbicides Plant Leaves Plant Proteins Sorghum Argentina Sorghum halepense Sorghum x almum
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1526498X_v68_n3_p430_VilaAiub
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_1526498X_v68_n3_p430_VilaAiub2023-10-03T16:21:07Z Glyphosate resistance in perennial Sorghum halepense (Johnsongrass), endowed by reduced glyphosate translocation and leaf uptake Vila-Aiub, M.M. Balbi, M.C. Distéfano, A.J. Fernández, L. Hopp, E. Yu, Q. Powles, S.B. Glyphosate leaf uptake Glyphosate translocation Non-target-site resistance mechanism Perennial Johnsongrass weed drug derivative glycine glyphosate herbicide vegetable protein assessment method biochemical composition biological uptake carbon isotope glyphosate molecular analysis pesticide resistance sorghum translocation article drug effect genetics herbicide resistance metabolism plant leaf sorghum transport at the cellular level Biological Transport Glycine Herbicide Resistance Herbicides Plant Leaves Plant Proteins Sorghum Argentina Sorghum halepense Sorghum x almum Background: In a large cropping area of northern Argentina, Sorghum halepense (Johnsongrass) has evolved towards glyphosate resistance. This study aimed to determine the molecular and biochemical basis conferring glyphosate resistance in this species. Experiments were conducted to assess target EPSPS gene sequences and 14C-glyphosate leaf absorption and translocation to meristematic tissues. Results: Individuals of all resistant (R) accessions exhibited significantly less glyphosate translocation to root (11% versus 29%) and stem (9% versus 26%) meristems when compared with susceptible (S) plants. A notably higher proportion of the applied glyphosate remained in the treated leaves of R plants (63%) than in the treated leaves of S plants (27%). In addition, individuals of S. halepense accession R 2 consistently showed lower glyphosate absorption rates in both adaxial (10-20%) and abaxial (20-25%) leaf surfaces compared with S plants. No glyphosate resistance endowing mutations in the EPSPS gene at Pro-101-106 residues were found in any of the evaluated R accessions. Conclusion: The results of the present investigation indicate that reduced glyphosate translocation to meristems is the primary mechanism endowing glyphosate resistance in S. halepense from cropping fields in Argentina. To a lesser extent, reduced glyphosate leaf uptake has also been shown to be involved in glyphosate-resistant S. halepense. © 2011 Society of Chemical Industry. Fil:Distéfano, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1526498X_v68_n3_p430_VilaAiub
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Glyphosate leaf uptake Glyphosate translocation Non-target-site resistance mechanism Perennial Johnsongrass weed drug derivative glycine glyphosate herbicide vegetable protein assessment method biochemical composition biological uptake carbon isotope glyphosate molecular analysis pesticide resistance sorghum translocation article drug effect genetics herbicide resistance metabolism plant leaf sorghum transport at the cellular level Biological Transport Glycine Herbicide Resistance Herbicides Plant Leaves Plant Proteins Sorghum Argentina Sorghum halepense Sorghum x almum
spellingShingle	Glyphosate leaf uptake Glyphosate translocation Non-target-site resistance mechanism Perennial Johnsongrass weed drug derivative glycine glyphosate herbicide vegetable protein assessment method biochemical composition biological uptake carbon isotope glyphosate molecular analysis pesticide resistance sorghum translocation article drug effect genetics herbicide resistance metabolism plant leaf sorghum transport at the cellular level Biological Transport Glycine Herbicide Resistance Herbicides Plant Leaves Plant Proteins Sorghum Argentina Sorghum halepense Sorghum x almum Vila-Aiub, M.M. Balbi, M.C. Distéfano, A.J. Fernández, L. Hopp, E. Yu, Q. Powles, S.B. Glyphosate resistance in perennial Sorghum halepense (Johnsongrass), endowed by reduced glyphosate translocation and leaf uptake
topic_facet	Glyphosate leaf uptake Glyphosate translocation Non-target-site resistance mechanism Perennial Johnsongrass weed drug derivative glycine glyphosate herbicide vegetable protein assessment method biochemical composition biological uptake carbon isotope glyphosate molecular analysis pesticide resistance sorghum translocation article drug effect genetics herbicide resistance metabolism plant leaf sorghum transport at the cellular level Biological Transport Glycine Herbicide Resistance Herbicides Plant Leaves Plant Proteins Sorghum Argentina Sorghum halepense Sorghum x almum
description	Background: In a large cropping area of northern Argentina, Sorghum halepense (Johnsongrass) has evolved towards glyphosate resistance. This study aimed to determine the molecular and biochemical basis conferring glyphosate resistance in this species. Experiments were conducted to assess target EPSPS gene sequences and 14C-glyphosate leaf absorption and translocation to meristematic tissues. Results: Individuals of all resistant (R) accessions exhibited significantly less glyphosate translocation to root (11% versus 29%) and stem (9% versus 26%) meristems when compared with susceptible (S) plants. A notably higher proportion of the applied glyphosate remained in the treated leaves of R plants (63%) than in the treated leaves of S plants (27%). In addition, individuals of S. halepense accession R 2 consistently showed lower glyphosate absorption rates in both adaxial (10-20%) and abaxial (20-25%) leaf surfaces compared with S plants. No glyphosate resistance endowing mutations in the EPSPS gene at Pro-101-106 residues were found in any of the evaluated R accessions. Conclusion: The results of the present investigation indicate that reduced glyphosate translocation to meristems is the primary mechanism endowing glyphosate resistance in S. halepense from cropping fields in Argentina. To a lesser extent, reduced glyphosate leaf uptake has also been shown to be involved in glyphosate-resistant S. halepense. © 2011 Society of Chemical Industry.
format	JOUR
author	Vila-Aiub, M.M. Balbi, M.C. Distéfano, A.J. Fernández, L. Hopp, E. Yu, Q. Powles, S.B.
author_facet	Vila-Aiub, M.M. Balbi, M.C. Distéfano, A.J. Fernández, L. Hopp, E. Yu, Q. Powles, S.B.
author_sort	Vila-Aiub, M.M.
title	Glyphosate resistance in perennial Sorghum halepense (Johnsongrass), endowed by reduced glyphosate translocation and leaf uptake
title_short	Glyphosate resistance in perennial Sorghum halepense (Johnsongrass), endowed by reduced glyphosate translocation and leaf uptake
title_full	Glyphosate resistance in perennial Sorghum halepense (Johnsongrass), endowed by reduced glyphosate translocation and leaf uptake
title_fullStr	Glyphosate resistance in perennial Sorghum halepense (Johnsongrass), endowed by reduced glyphosate translocation and leaf uptake
title_full_unstemmed	Glyphosate resistance in perennial Sorghum halepense (Johnsongrass), endowed by reduced glyphosate translocation and leaf uptake
title_sort	glyphosate resistance in perennial sorghum halepense (johnsongrass), endowed by reduced glyphosate translocation and leaf uptake
url	http://hdl.handle.net/20.500.12110/paper_1526498X_v68_n3_p430_VilaAiub
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_version_	1807323666973720576

Glyphosate resistance in perennial Sorghum halepense (Johnsongrass), endowed by reduced glyphosate translocation and leaf uptake

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