Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit
Cochlear inner hair cells (IHCs) release neurotransmitter onto afferent auditory nerve fibers in response to sound stimulation. During early development, synaptic transmission is triggered by spontaneous Ca2+ spikes which are modulated by an efferent cholinergic innervation to IHCs. This synapse is...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_15253961_v10_n2_p221_GomezCasati |
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todo:paper_15253961_v10_n2_p221_GomezCasati2023-10-03T16:20:59Z Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit Gómez-Casati, M.E. Wedemeyer, C. Taranda, J. Lipovsek, M. Dalamon, V. Elgoyhen, A.B. Katz, E. Auditory Calcium spikes Calcium-sensitive K+ channels Cholinergic Development Voltage-gated K+ channels calcium calcium activated potassium channel calcium channel cholinergic receptor neurotransmitter nicotinic receptor potassium channel voltage gated potassium channel acoustic nerve fiber animal cell article auditory stimulation calcium conductance calcium transport Chrna10 gene cochlea controlled study Corti organ electric potential electrophysiology gene hair cell hearing mouse neurotransmitter release newborn nonhuman parasympathetic innervation priority journal sensory nerve synapse synaptic transmission whole cell Animals Apamin Cochlea Electric Capacitance Hair Cells, Auditory, Inner Hearing Mice Patch-Clamp Techniques Potassium Channels, Calcium-Activated Receptors, Nicotinic Cochlear inner hair cells (IHCs) release neurotransmitter onto afferent auditory nerve fibers in response to sound stimulation. During early development, synaptic transmission is triggered by spontaneous Ca2+ spikes which are modulated by an efferent cholinergic innervation to IHCs. This synapse is inhibitory and mediated by the α9α10 nicotinic cholinergic receptor (nAChR). After the onset of hearing, large-conductance Ca2+-activated K+ channels are acquired and both the spiking activity and the efferent innervation disappear from IHCs. In this work, we studied the developmental changes in the membrane properties of cochlear IHCs from α10 nAChR gene (Chrna10) "knockout" mice. Electrophysiological properties of IHCs were studied by whole-cell recordings in acutely excised apical turns of the organ of Corti from developing mice. Neither the spiking activity nor the developmental functional expression of voltage-gated and/or calcium-sensitive K+ channels is altered in the absence of the α10 nAChR subunit. The present results show that the α10 nAChR subunit is not essential for the correct establishment of the intrinsic electrical properties of IHCs during development. © 2009 Association for Research in Otolaryngology. Fil:Gómez-Casati, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wedemeyer, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Taranda, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lipovsek, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15253961_v10_n2_p221_GomezCasati |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Auditory Calcium spikes Calcium-sensitive K+ channels Cholinergic Development Voltage-gated K+ channels calcium calcium activated potassium channel calcium channel cholinergic receptor neurotransmitter nicotinic receptor potassium channel voltage gated potassium channel acoustic nerve fiber animal cell article auditory stimulation calcium conductance calcium transport Chrna10 gene cochlea controlled study Corti organ electric potential electrophysiology gene hair cell hearing mouse neurotransmitter release newborn nonhuman parasympathetic innervation priority journal sensory nerve synapse synaptic transmission whole cell Animals Apamin Cochlea Electric Capacitance Hair Cells, Auditory, Inner Hearing Mice Patch-Clamp Techniques Potassium Channels, Calcium-Activated Receptors, Nicotinic |
| spellingShingle |
Auditory Calcium spikes Calcium-sensitive K+ channels Cholinergic Development Voltage-gated K+ channels calcium calcium activated potassium channel calcium channel cholinergic receptor neurotransmitter nicotinic receptor potassium channel voltage gated potassium channel acoustic nerve fiber animal cell article auditory stimulation calcium conductance calcium transport Chrna10 gene cochlea controlled study Corti organ electric potential electrophysiology gene hair cell hearing mouse neurotransmitter release newborn nonhuman parasympathetic innervation priority journal sensory nerve synapse synaptic transmission whole cell Animals Apamin Cochlea Electric Capacitance Hair Cells, Auditory, Inner Hearing Mice Patch-Clamp Techniques Potassium Channels, Calcium-Activated Receptors, Nicotinic Gómez-Casati, M.E. Wedemeyer, C. Taranda, J. Lipovsek, M. Dalamon, V. Elgoyhen, A.B. Katz, E. Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit |
| topic_facet |
Auditory Calcium spikes Calcium-sensitive K+ channels Cholinergic Development Voltage-gated K+ channels calcium calcium activated potassium channel calcium channel cholinergic receptor neurotransmitter nicotinic receptor potassium channel voltage gated potassium channel acoustic nerve fiber animal cell article auditory stimulation calcium conductance calcium transport Chrna10 gene cochlea controlled study Corti organ electric potential electrophysiology gene hair cell hearing mouse neurotransmitter release newborn nonhuman parasympathetic innervation priority journal sensory nerve synapse synaptic transmission whole cell Animals Apamin Cochlea Electric Capacitance Hair Cells, Auditory, Inner Hearing Mice Patch-Clamp Techniques Potassium Channels, Calcium-Activated Receptors, Nicotinic |
| description |
Cochlear inner hair cells (IHCs) release neurotransmitter onto afferent auditory nerve fibers in response to sound stimulation. During early development, synaptic transmission is triggered by spontaneous Ca2+ spikes which are modulated by an efferent cholinergic innervation to IHCs. This synapse is inhibitory and mediated by the α9α10 nicotinic cholinergic receptor (nAChR). After the onset of hearing, large-conductance Ca2+-activated K+ channels are acquired and both the spiking activity and the efferent innervation disappear from IHCs. In this work, we studied the developmental changes in the membrane properties of cochlear IHCs from α10 nAChR gene (Chrna10) "knockout" mice. Electrophysiological properties of IHCs were studied by whole-cell recordings in acutely excised apical turns of the organ of Corti from developing mice. Neither the spiking activity nor the developmental functional expression of voltage-gated and/or calcium-sensitive K+ channels is altered in the absence of the α10 nAChR subunit. The present results show that the α10 nAChR subunit is not essential for the correct establishment of the intrinsic electrical properties of IHCs during development. © 2009 Association for Research in Otolaryngology. |
| format |
JOUR |
| author |
Gómez-Casati, M.E. Wedemeyer, C. Taranda, J. Lipovsek, M. Dalamon, V. Elgoyhen, A.B. Katz, E. |
| author_facet |
Gómez-Casati, M.E. Wedemeyer, C. Taranda, J. Lipovsek, M. Dalamon, V. Elgoyhen, A.B. Katz, E. |
| author_sort |
Gómez-Casati, M.E. |
| title |
Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit |
| title_short |
Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit |
| title_full |
Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit |
| title_fullStr |
Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit |
| title_full_unstemmed |
Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit |
| title_sort |
electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the α10 nicotinic cholinergic receptor subunit |
| url |
http://hdl.handle.net/20.500.12110/paper_15253961_v10_n2_p221_GomezCasati |
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