Dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy
Fluorescence fluctuation-based methods are non-invasive microscopy tools especially suited for the study of dynamical aspects of biological processes. These methods examine spontaneous intensity fluctuations produced by fluorescent molecules moving through the small, femtoliter-sized observation vol...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_15216543_v69_n1_p8_GonzalezBardeci |
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todo:paper_15216543_v69_n1_p8_GonzalezBardeci2023-10-03T16:20:39Z Dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy González Bardeci, N. Angiolini, J.F. De Rossi, M.C. Bruno, L. Levi, V. autocorrelation cross-correlation diffusion fluorescence correlation spectroscopy fluorescence microscopy intracellular dynamics cell nucleus confocal microscopy cytology fluctuations technique fluorescence correlation microscopy fluorescence cross correlation spectroscopy fluorescence microscopy molecule nonhuman practice guideline quantitative analysis Review cells cytoplasm fluorescence molecular imaging procedures transport at the cellular level ultrastructure Biological Transport Cells Cytoplasm Fluorescence Microscopy, Fluorescence Molecular Imaging Fluorescence fluctuation-based methods are non-invasive microscopy tools especially suited for the study of dynamical aspects of biological processes. These methods examine spontaneous intensity fluctuations produced by fluorescent molecules moving through the small, femtoliter-sized observation volume defined in confocal and multiphoton microscopes. The quantitative analysis of the intensity trace provides information on the processes producing the fluctuations that include diffusion, binding interactions, chemical reactions and photophysical phenomena. In this review, we present the basic principles of the most widespread fluctuation-based methods, discuss their implementation in standard confocal microscopes and briefly revise some examples of their applications to address relevant questions in living cells. The ultimate goal of these methods in the Cell Biology field is to observe biomolecules as they move, interact with targets and perform their biological action in the natural context. © 2016 IUBMB Life, 69(1):8–15, 2017. © 2016 International Union of Biochemistry and Molecular Biology Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_15216543_v69_n1_p8_GonzalezBardeci |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
autocorrelation cross-correlation diffusion fluorescence correlation spectroscopy fluorescence microscopy intracellular dynamics cell nucleus confocal microscopy cytology fluctuations technique fluorescence correlation microscopy fluorescence cross correlation spectroscopy fluorescence microscopy molecule nonhuman practice guideline quantitative analysis Review cells cytoplasm fluorescence molecular imaging procedures transport at the cellular level ultrastructure Biological Transport Cells Cytoplasm Fluorescence Microscopy, Fluorescence Molecular Imaging |
| spellingShingle |
autocorrelation cross-correlation diffusion fluorescence correlation spectroscopy fluorescence microscopy intracellular dynamics cell nucleus confocal microscopy cytology fluctuations technique fluorescence correlation microscopy fluorescence cross correlation spectroscopy fluorescence microscopy molecule nonhuman practice guideline quantitative analysis Review cells cytoplasm fluorescence molecular imaging procedures transport at the cellular level ultrastructure Biological Transport Cells Cytoplasm Fluorescence Microscopy, Fluorescence Molecular Imaging González Bardeci, N. Angiolini, J.F. De Rossi, M.C. Bruno, L. Levi, V. Dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy |
| topic_facet |
autocorrelation cross-correlation diffusion fluorescence correlation spectroscopy fluorescence microscopy intracellular dynamics cell nucleus confocal microscopy cytology fluctuations technique fluorescence correlation microscopy fluorescence cross correlation spectroscopy fluorescence microscopy molecule nonhuman practice guideline quantitative analysis Review cells cytoplasm fluorescence molecular imaging procedures transport at the cellular level ultrastructure Biological Transport Cells Cytoplasm Fluorescence Microscopy, Fluorescence Molecular Imaging |
| description |
Fluorescence fluctuation-based methods are non-invasive microscopy tools especially suited for the study of dynamical aspects of biological processes. These methods examine spontaneous intensity fluctuations produced by fluorescent molecules moving through the small, femtoliter-sized observation volume defined in confocal and multiphoton microscopes. The quantitative analysis of the intensity trace provides information on the processes producing the fluctuations that include diffusion, binding interactions, chemical reactions and photophysical phenomena. In this review, we present the basic principles of the most widespread fluctuation-based methods, discuss their implementation in standard confocal microscopes and briefly revise some examples of their applications to address relevant questions in living cells. The ultimate goal of these methods in the Cell Biology field is to observe biomolecules as they move, interact with targets and perform their biological action in the natural context. © 2016 IUBMB Life, 69(1):8–15, 2017. © 2016 International Union of Biochemistry and Molecular Biology |
| format |
JOUR |
| author |
González Bardeci, N. Angiolini, J.F. De Rossi, M.C. Bruno, L. Levi, V. |
| author_facet |
González Bardeci, N. Angiolini, J.F. De Rossi, M.C. Bruno, L. Levi, V. |
| author_sort |
González Bardeci, N. |
| title |
Dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy |
| title_short |
Dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy |
| title_full |
Dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy |
| title_fullStr |
Dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy |
| title_full_unstemmed |
Dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy |
| title_sort |
dynamics of intracellular processes in live-cell systems unveiled by fluorescence correlation microscopy |
| url |
http://hdl.handle.net/20.500.12110/paper_15216543_v69_n1_p8_GonzalezBardeci |
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