Luminal Ca2+ dynamics during IP3R mediated signals

The role of cytosolic Ca2+ on the kinetics of Inositol 1,4,5-triphosphate receptors (IP3Rs) and on the dynamics of IP3R-mediated Ca2+ signals has been studied at large both experimentally and by modeling. The role of luminal Ca2+ has not been investigated with that much detail although it has been f...

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Detalles Bibliográficos
Autores principales:	Lopez, L.F., Dawson, S.P.
Formato:	JOUR
Materias:	calcium fluorescent dye inositol 1,4,5 trisphosphate receptor animal calcium signaling chemistry cytosol metabolism oocyte Xenopus laevis Animals Calcium Calcium Signaling Cytosol Fluorescent Dyes Inositol 1,4,5-Trisphosphate Receptors Oocytes
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_14783967_v13_n3_p_Lopez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_14783967_v13_n3_p_Lopez
record_format	dspace
spelling	todo:paper_14783967_v13_n3_p_Lopez2023-10-03T16:19:24Z Luminal Ca2+ dynamics during IP3R mediated signals Lopez, L.F. Dawson, S.P. calcium fluorescent dye inositol 1,4,5 trisphosphate receptor animal calcium signaling chemistry cytosol metabolism oocyte Xenopus laevis Animals Calcium Calcium Signaling Cytosol Fluorescent Dyes Inositol 1,4,5-Trisphosphate Receptors Oocytes Xenopus laevis The role of cytosolic Ca2+ on the kinetics of Inositol 1,4,5-triphosphate receptors (IP3Rs) and on the dynamics of IP3R-mediated Ca2+ signals has been studied at large both experimentally and by modeling. The role of luminal Ca2+ has not been investigated with that much detail although it has been found that it is relevant for signal termination in the case of Ca2+ release through ryanodine receptors. In this work we present the results of observing the dynamics of luminal and cytosolic Ca2+ simultaneously in Xenopus laevis oocytes. Combining observations and modeling we conclude that there is a rapid mechanism that guarantees the availability of free Ca2+ in the lumen even when a relatively large Ca2+ release is evoked. Comparing the dynamics of cytosolic and luminal Ca2+ during a release, we estimate that they are consistent with a 80% of luminal Ca2+ being buffered. The rapid availability of free luminal Ca2+ correlates with the observation that the lumen occupies a considerable volume in several regions across the images. © 2016 IOP Publishing Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14783967_v13_n3_p_Lopez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	calcium fluorescent dye inositol 1,4,5 trisphosphate receptor animal calcium signaling chemistry cytosol metabolism oocyte Xenopus laevis Animals Calcium Calcium Signaling Cytosol Fluorescent Dyes Inositol 1,4,5-Trisphosphate Receptors Oocytes Xenopus laevis
spellingShingle	calcium fluorescent dye inositol 1,4,5 trisphosphate receptor animal calcium signaling chemistry cytosol metabolism oocyte Xenopus laevis Animals Calcium Calcium Signaling Cytosol Fluorescent Dyes Inositol 1,4,5-Trisphosphate Receptors Oocytes Xenopus laevis Lopez, L.F. Dawson, S.P. Luminal Ca2+ dynamics during IP3R mediated signals
topic_facet	calcium fluorescent dye inositol 1,4,5 trisphosphate receptor animal calcium signaling chemistry cytosol metabolism oocyte Xenopus laevis Animals Calcium Calcium Signaling Cytosol Fluorescent Dyes Inositol 1,4,5-Trisphosphate Receptors Oocytes Xenopus laevis
description	The role of cytosolic Ca2+ on the kinetics of Inositol 1,4,5-triphosphate receptors (IP3Rs) and on the dynamics of IP3R-mediated Ca2+ signals has been studied at large both experimentally and by modeling. The role of luminal Ca2+ has not been investigated with that much detail although it has been found that it is relevant for signal termination in the case of Ca2+ release through ryanodine receptors. In this work we present the results of observing the dynamics of luminal and cytosolic Ca2+ simultaneously in Xenopus laevis oocytes. Combining observations and modeling we conclude that there is a rapid mechanism that guarantees the availability of free Ca2+ in the lumen even when a relatively large Ca2+ release is evoked. Comparing the dynamics of cytosolic and luminal Ca2+ during a release, we estimate that they are consistent with a 80% of luminal Ca2+ being buffered. The rapid availability of free luminal Ca2+ correlates with the observation that the lumen occupies a considerable volume in several regions across the images. © 2016 IOP Publishing Ltd.
format	JOUR
author	Lopez, L.F. Dawson, S.P.
author_facet	Lopez, L.F. Dawson, S.P.
author_sort	Lopez, L.F.
title	Luminal Ca2+ dynamics during IP3R mediated signals
title_short	Luminal Ca2+ dynamics during IP3R mediated signals
title_full	Luminal Ca2+ dynamics during IP3R mediated signals
title_fullStr	Luminal Ca2+ dynamics during IP3R mediated signals
title_full_unstemmed	Luminal Ca2+ dynamics during IP3R mediated signals
title_sort	luminal ca2+ dynamics during ip3r mediated signals
url	http://hdl.handle.net/20.500.12110/paper_14783967_v13_n3_p_Lopez
work_keys_str_mv	AT lopezlf luminalca2dynamicsduringip3rmediatedsignals AT dawsonsp luminalca2dynamicsduringip3rmediatedsignals
_version_	1807320478839209984

Luminal Ca2+ dynamics during IP3R mediated signals

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