Mapping the protein-binding sites for iridium(III)-based CO-releasing molecules

A combination of mass spectrometry, Raman microspectroscopy, circular dichroism and X-ray crystallography has been used to obtain detailed information on the reaction of an iridium-based CO-releasing molecule (Ir-CORM), Cs2IrCl5CO, with a model protein, bovine pancreatic ribonuclease. The results sh...

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Autores principales:	Caterino, M., Petruk, A.A., Vergara, A., Ferraro, G., Marasco, D., Doctorovich, F., Estrin, D.A., Merlino, A.
Formato:	JOUR
Materias:	Amino acids Binding sites Bins Biochemistry Crystallography Dichroism Iridium Mass spectrometry Molecules Proteins Biological properties Bovine pancreatic ribonuclease Model proteins N-terminals Protein targets Protein-binding sites Raman microspectroscopy Side-chains X ray crystallography carbon monoxide iridium protein protein binding binding site chemical structure chemistry circular dichroism mass spectrometry Raman spectrometry Binding Sites Carbon Monoxide Circular Dichroism Crystallography, X-Ray Mass Spectrometry Molecular Structure Protein Binding Spectrum Analysis, Raman
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_14779226_v45_n30_p12206_Caterino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_14779226_v45_n30_p12206_Caterino
record_format	dspace
spelling	todo:paper_14779226_v45_n30_p12206_Caterino2023-10-03T16:19:19Z Mapping the protein-binding sites for iridium(III)-based CO-releasing molecules Caterino, M. Petruk, A.A. Vergara, A. Ferraro, G. Marasco, D. Doctorovich, F. Estrin, D.A. Merlino, A. Amino acids Binding sites Bins Biochemistry Crystallography Dichroism Iridium Mass spectrometry Molecules Proteins Biological properties Bovine pancreatic ribonuclease Model proteins N-terminals Protein targets Protein-binding sites Raman microspectroscopy Side-chains X ray crystallography carbon monoxide iridium protein protein binding binding site chemical structure chemistry circular dichroism mass spectrometry Raman spectrometry X ray crystallography Binding Sites Carbon Monoxide Circular Dichroism Crystallography, X-Ray Iridium Mass Spectrometry Molecular Structure Protein Binding Proteins Spectrum Analysis, Raman A combination of mass spectrometry, Raman microspectroscopy, circular dichroism and X-ray crystallography has been used to obtain detailed information on the reaction of an iridium-based CO-releasing molecule (Ir-CORM), Cs2IrCl5CO, with a model protein, bovine pancreatic ribonuclease. The results show that Ir-compound fragments bind to the N-terminal amine and close to histidine and methionine side chains, and the CO ligand is retained for a long time. The data provide helpful information for identifying protein targets for Ir-CORMs and for studying the mechanism that allows them to exhibit their interesting biological properties. © The Royal Society of Chemistry 2016. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14779226_v45_n30_p12206_Caterino
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Amino acids Binding sites Bins Biochemistry Crystallography Dichroism Iridium Mass spectrometry Molecules Proteins Biological properties Bovine pancreatic ribonuclease Model proteins N-terminals Protein targets Protein-binding sites Raman microspectroscopy Side-chains X ray crystallography carbon monoxide iridium protein protein binding binding site chemical structure chemistry circular dichroism mass spectrometry Raman spectrometry X ray crystallography Binding Sites Carbon Monoxide Circular Dichroism Crystallography, X-Ray Iridium Mass Spectrometry Molecular Structure Protein Binding Proteins Spectrum Analysis, Raman
spellingShingle	Amino acids Binding sites Bins Biochemistry Crystallography Dichroism Iridium Mass spectrometry Molecules Proteins Biological properties Bovine pancreatic ribonuclease Model proteins N-terminals Protein targets Protein-binding sites Raman microspectroscopy Side-chains X ray crystallography carbon monoxide iridium protein protein binding binding site chemical structure chemistry circular dichroism mass spectrometry Raman spectrometry X ray crystallography Binding Sites Carbon Monoxide Circular Dichroism Crystallography, X-Ray Iridium Mass Spectrometry Molecular Structure Protein Binding Proteins Spectrum Analysis, Raman Caterino, M. Petruk, A.A. Vergara, A. Ferraro, G. Marasco, D. Doctorovich, F. Estrin, D.A. Merlino, A. Mapping the protein-binding sites for iridium(III)-based CO-releasing molecules
topic_facet	Amino acids Binding sites Bins Biochemistry Crystallography Dichroism Iridium Mass spectrometry Molecules Proteins Biological properties Bovine pancreatic ribonuclease Model proteins N-terminals Protein targets Protein-binding sites Raman microspectroscopy Side-chains X ray crystallography carbon monoxide iridium protein protein binding binding site chemical structure chemistry circular dichroism mass spectrometry Raman spectrometry X ray crystallography Binding Sites Carbon Monoxide Circular Dichroism Crystallography, X-Ray Iridium Mass Spectrometry Molecular Structure Protein Binding Proteins Spectrum Analysis, Raman
description	A combination of mass spectrometry, Raman microspectroscopy, circular dichroism and X-ray crystallography has been used to obtain detailed information on the reaction of an iridium-based CO-releasing molecule (Ir-CORM), Cs2IrCl5CO, with a model protein, bovine pancreatic ribonuclease. The results show that Ir-compound fragments bind to the N-terminal amine and close to histidine and methionine side chains, and the CO ligand is retained for a long time. The data provide helpful information for identifying protein targets for Ir-CORMs and for studying the mechanism that allows them to exhibit their interesting biological properties. © The Royal Society of Chemistry 2016.
format	JOUR
author	Caterino, M. Petruk, A.A. Vergara, A. Ferraro, G. Marasco, D. Doctorovich, F. Estrin, D.A. Merlino, A.
author_facet	Caterino, M. Petruk, A.A. Vergara, A. Ferraro, G. Marasco, D. Doctorovich, F. Estrin, D.A. Merlino, A.
author_sort	Caterino, M.
title	Mapping the protein-binding sites for iridium(III)-based CO-releasing molecules
title_short	Mapping the protein-binding sites for iridium(III)-based CO-releasing molecules
title_full	Mapping the protein-binding sites for iridium(III)-based CO-releasing molecules
title_fullStr	Mapping the protein-binding sites for iridium(III)-based CO-releasing molecules
title_full_unstemmed	Mapping the protein-binding sites for iridium(III)-based CO-releasing molecules
title_sort	mapping the protein-binding sites for iridium(iii)-based co-releasing molecules
url	http://hdl.handle.net/20.500.12110/paper_14779226_v45_n30_p12206_Caterino
work_keys_str_mv	AT caterinom mappingtheproteinbindingsitesforiridiumiiibasedcoreleasingmolecules AT petrukaa mappingtheproteinbindingsitesforiridiumiiibasedcoreleasingmolecules AT vergaraa mappingtheproteinbindingsitesforiridiumiiibasedcoreleasingmolecules AT ferrarog mappingtheproteinbindingsitesforiridiumiiibasedcoreleasingmolecules AT marascod mappingtheproteinbindingsitesforiridiumiiibasedcoreleasingmolecules AT doctorovichf mappingtheproteinbindingsitesforiridiumiiibasedcoreleasingmolecules AT estrinda mappingtheproteinbindingsitesforiridiumiiibasedcoreleasingmolecules AT merlinoa mappingtheproteinbindingsitesforiridiumiiibasedcoreleasingmolecules
_version_	1807321622485401600

Mapping the protein-binding sites for iridium(III)-based CO-releasing molecules

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