Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos
Background: BMP4 is a member of the transforming growth factor beta (TGFbeta) superfamily and Noggin is a potent BMP inhibitor that exerts its function by binding to BMPs preventing interactions with its receptors. The aim of this work was to investigate the role of BMP4 and Noggin, on oocytes in vi...
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todo:paper_14777827_v9_n_p_LaRosa2023-10-03T16:19:12Z Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos La Rosa, I. Camargo, L.S.A. Pereira, M.M. Fernandez-Martin, R. Paz, D.A. Salamone, D.F. bone morphogenetic protein 4 cell protein growth differentiation factor 9 heat shock protein 70 mater protein noggin octamer transcription factor 4 protein Bax transforming growth factor beta unclassified drug ZAR1 protein autoantigen carrier protein heat shock protein 70 noggin protein animal cell article blastocyst cell nucleus controlled study cow embryo culture embryo development fertilization in vitro gene expression hatching immunocytochemistry maturation meiosis nonhuman oocyte maturation parthenogenesis preimplantation embryo reverse transcription polymerase chain reaction animal biosynthesis cattle drug antagonism drug effect metabolism physiology Bovinae Animals Autoantigens Blastocyst Bone Morphogenetic Protein 4 Carrier Proteins Cattle Embryonic Development Fertilization in Vitro HSP70 Heat-Shock Proteins Parthenogenesis Background: BMP4 is a member of the transforming growth factor beta (TGFbeta) superfamily and Noggin is a potent BMP inhibitor that exerts its function by binding to BMPs preventing interactions with its receptors. The aim of this work was to investigate the role of BMP4 and Noggin, on oocytes in vitro maturation (m experiments) and embryos in vitro development (c experiments) of bovine.Methods: For m experiments, COCs were collected from slaughterhouse ovaries and in vitro matured in TCM with 100 ng/ml of either BMP4 or Noggin. After 24 h, the nuclear stage of the oocytes was determined by staining with Hoechst 33342. In addition, RT-qPCR was performed on MII oocytes to study the relative concentration of ZAR1, GDF9, BAX, MATER and HSP70 transcripts. Treated oocytes were submitted to parthenogenic activation (PA) or in vitro fertilization (IVF) and cultured in CR2. For c experiments, non-treated matured oocytes were submitted to PA or IVF to generate embryos that were exposed to 100 ng/ml of BMP4 or Noggin in CR2 until day nine of culture. Cleavage, blastocyst and hatching rates, expression pattern of the transcription factor Oct-4 in blastocysts and embryo cell number at day two and nine post-activation or fertilization were evaluated.Results: We found that Noggin, as BMP4, did not affect oocyte nuclear maturation. Noggin supplementation up-regulated the expression of HSP70 and MATER genes in matured oocytes. Moreover, BMP4 during maturation increased the proportion of Oct-4 positive cells in parthenogenic embryos. On the other hand, when Noggin was added to embryo culture medium, developmental rates of parthenogenic and in vitro fertilized embryos were reduced. However, BMP4 addition decreases the development only for in vitro fertilized embryos. BMP4 and Noggin during culture reduced the proportion of Oct-4-expressing cells.Conclusions: Our results show that BMP4 is implicated in bovine oocytes maturation and embryo development. Moreover, our findings demonstrate, for the first time, that a correct balance of BMP signaling is needed for proper pre-implantation development of bovine embryos. © 2011 La Rosa et al; licensee BioMed Central Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14777827_v9_n_p_LaRosa |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bone morphogenetic protein 4 cell protein growth differentiation factor 9 heat shock protein 70 mater protein noggin octamer transcription factor 4 protein Bax transforming growth factor beta unclassified drug ZAR1 protein autoantigen carrier protein heat shock protein 70 noggin protein animal cell article blastocyst cell nucleus controlled study cow embryo culture embryo development fertilization in vitro gene expression hatching immunocytochemistry maturation meiosis nonhuman oocyte maturation parthenogenesis preimplantation embryo reverse transcription polymerase chain reaction animal biosynthesis cattle drug antagonism drug effect metabolism physiology Bovinae Animals Autoantigens Blastocyst Bone Morphogenetic Protein 4 Carrier Proteins Cattle Embryonic Development Fertilization in Vitro HSP70 Heat-Shock Proteins Parthenogenesis |
| spellingShingle |
bone morphogenetic protein 4 cell protein growth differentiation factor 9 heat shock protein 70 mater protein noggin octamer transcription factor 4 protein Bax transforming growth factor beta unclassified drug ZAR1 protein autoantigen carrier protein heat shock protein 70 noggin protein animal cell article blastocyst cell nucleus controlled study cow embryo culture embryo development fertilization in vitro gene expression hatching immunocytochemistry maturation meiosis nonhuman oocyte maturation parthenogenesis preimplantation embryo reverse transcription polymerase chain reaction animal biosynthesis cattle drug antagonism drug effect metabolism physiology Bovinae Animals Autoantigens Blastocyst Bone Morphogenetic Protein 4 Carrier Proteins Cattle Embryonic Development Fertilization in Vitro HSP70 Heat-Shock Proteins Parthenogenesis La Rosa, I. Camargo, L.S.A. Pereira, M.M. Fernandez-Martin, R. Paz, D.A. Salamone, D.F. Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos |
| topic_facet |
bone morphogenetic protein 4 cell protein growth differentiation factor 9 heat shock protein 70 mater protein noggin octamer transcription factor 4 protein Bax transforming growth factor beta unclassified drug ZAR1 protein autoantigen carrier protein heat shock protein 70 noggin protein animal cell article blastocyst cell nucleus controlled study cow embryo culture embryo development fertilization in vitro gene expression hatching immunocytochemistry maturation meiosis nonhuman oocyte maturation parthenogenesis preimplantation embryo reverse transcription polymerase chain reaction animal biosynthesis cattle drug antagonism drug effect metabolism physiology Bovinae Animals Autoantigens Blastocyst Bone Morphogenetic Protein 4 Carrier Proteins Cattle Embryonic Development Fertilization in Vitro HSP70 Heat-Shock Proteins Parthenogenesis |
| description |
Background: BMP4 is a member of the transforming growth factor beta (TGFbeta) superfamily and Noggin is a potent BMP inhibitor that exerts its function by binding to BMPs preventing interactions with its receptors. The aim of this work was to investigate the role of BMP4 and Noggin, on oocytes in vitro maturation (m experiments) and embryos in vitro development (c experiments) of bovine.Methods: For m experiments, COCs were collected from slaughterhouse ovaries and in vitro matured in TCM with 100 ng/ml of either BMP4 or Noggin. After 24 h, the nuclear stage of the oocytes was determined by staining with Hoechst 33342. In addition, RT-qPCR was performed on MII oocytes to study the relative concentration of ZAR1, GDF9, BAX, MATER and HSP70 transcripts. Treated oocytes were submitted to parthenogenic activation (PA) or in vitro fertilization (IVF) and cultured in CR2. For c experiments, non-treated matured oocytes were submitted to PA or IVF to generate embryos that were exposed to 100 ng/ml of BMP4 or Noggin in CR2 until day nine of culture. Cleavage, blastocyst and hatching rates, expression pattern of the transcription factor Oct-4 in blastocysts and embryo cell number at day two and nine post-activation or fertilization were evaluated.Results: We found that Noggin, as BMP4, did not affect oocyte nuclear maturation. Noggin supplementation up-regulated the expression of HSP70 and MATER genes in matured oocytes. Moreover, BMP4 during maturation increased the proportion of Oct-4 positive cells in parthenogenic embryos. On the other hand, when Noggin was added to embryo culture medium, developmental rates of parthenogenic and in vitro fertilized embryos were reduced. However, BMP4 addition decreases the development only for in vitro fertilized embryos. BMP4 and Noggin during culture reduced the proportion of Oct-4-expressing cells.Conclusions: Our results show that BMP4 is implicated in bovine oocytes maturation and embryo development. Moreover, our findings demonstrate, for the first time, that a correct balance of BMP signaling is needed for proper pre-implantation development of bovine embryos. © 2011 La Rosa et al; licensee BioMed Central Ltd. |
| format |
JOUR |
| author |
La Rosa, I. Camargo, L.S.A. Pereira, M.M. Fernandez-Martin, R. Paz, D.A. Salamone, D.F. |
| author_facet |
La Rosa, I. Camargo, L.S.A. Pereira, M.M. Fernandez-Martin, R. Paz, D.A. Salamone, D.F. |
| author_sort |
La Rosa, I. |
| title |
Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos |
| title_short |
Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos |
| title_full |
Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos |
| title_fullStr |
Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos |
| title_full_unstemmed |
Effects of bone morphogenic protein 4 (BMP4) and its inhibitor, Noggin, on in vitro maturation and culture of bovine preimplantation embryos |
| title_sort |
effects of bone morphogenic protein 4 (bmp4) and its inhibitor, noggin, on in vitro maturation and culture of bovine preimplantation embryos |
| url |
http://hdl.handle.net/20.500.12110/paper_14777827_v9_n_p_LaRosa |
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