Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation

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The aim of this study was to evaluate the DNA damage induced by low and high linear energy transfer radiation in two related cell lines with different radiation sensitivity, CHO10B2 and irs-20 cells (defective in DNAPKcs). Double-strand breaks were assessed by the detection of phosphorylated histone...

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Autores principales: Bracalente, C., Ibañez, I.L., Molinari, B., Palmieri, M.A., Maglioco, A., Policastro, L., Kreiner, A.J., Burlón, A., Valda, A., Davidson, J., Davidson, M., Vázquez, M., Ozafrán, M., Durán, H.
Formato: JOUR
Materias:
γH2AX
CHO10B2
DNA double strand break
DNA-PKcs
H2AX foci size
Ionising radiation
irs-20
LET
Linear energy transfer
histone gamma h2ax
histone H2AX
lithium
unclassified drug
animal cell
article
controlled study
DNA damage
double stranded DNA break
gamma irradiation
immunofluorescence
ionizing radiation
linear energy transfer
nonhuman
protein phosphorylation
radiation dose fractionation
radiosensitivity
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_14776545_v7_n5_p393_Bracalente
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_14776545_v7_n5_p393_Bracalente
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spelling todo:paper_14776545_v7_n5_p393_Bracalente2023-10-03T16:19:12Z Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation Bracalente, C. Ibañez, I.L. Molinari, B. Palmieri, M.A. Maglioco, A. Policastro, L. Kreiner, A.J. Burlón, A. Valda, A. Davidson, J. Davidson, M. Vázquez, M. Ozafrán, M. Durán, H. γH2AX CHO10B2 DNA double strand break DNA-PKcs H2AX foci size Ionising radiation irs-20 LET Linear energy transfer histone gamma h2ax histone H2AX lithium unclassified drug animal cell article controlled study DNA damage double stranded DNA break gamma irradiation immunofluorescence ionizing radiation linear energy transfer nonhuman protein phosphorylation radiation dose fractionation radiosensitivity The aim of this study was to evaluate the DNA damage induced by low and high linear energy transfer radiation in two related cell lines with different radiation sensitivity, CHO10B2 and irs-20 cells (defective in DNAPKcs). Double-strand breaks were assessed by the detection of phosphorylated histone H2AX foci (γH2AX). The number of foci increased as a function of dose for both cell lines and types of radiation. However, irs-20 cells showed a higher number of foci than the parental CHO10B2. An increase in the foci size was observed for both cell lines after lithium irradiation. This could be attributed to clusters of DNA damage. Moreover, the number of lithiuminduced larger foci per nuclei increased with dose and fitted with the expected number of hits per nucleus. Concluding, γH2AX foci size provides a potential tool to characterise the intrinsic radiosensitivity of cells related to DNA damage and to compare the effects of different quality radiations. Copyright © 2010 Inderscience Enterprises Ltd. Fil:Molinari, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Palmieri, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maglioco, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Policastro, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kreiner, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Burlón, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Davidson, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Davidson, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Durán, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14776545_v7_n5_p393_Bracalente
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic γH2AX
CHO10B2
DNA double strand break
DNA-PKcs
H2AX foci size
Ionising radiation
irs-20
LET
Linear energy transfer
histone gamma h2ax
histone H2AX
lithium
unclassified drug
animal cell
article
controlled study
DNA damage
double stranded DNA break
gamma irradiation
immunofluorescence
ionizing radiation
linear energy transfer
nonhuman
protein phosphorylation
radiation dose fractionation
radiosensitivity
spellingShingle γH2AX
CHO10B2
DNA double strand break
DNA-PKcs
H2AX foci size
Ionising radiation
irs-20
LET
Linear energy transfer
histone gamma h2ax
histone H2AX
lithium
unclassified drug
animal cell
article
controlled study
DNA damage
double stranded DNA break
gamma irradiation
immunofluorescence
ionizing radiation
linear energy transfer
nonhuman
protein phosphorylation
radiation dose fractionation
radiosensitivity
Bracalente, C.
Ibañez, I.L.
Molinari, B.
Palmieri, M.A.
Maglioco, A.
Policastro, L.
Kreiner, A.J.
Burlón, A.
Valda, A.
Davidson, J.
Davidson, M.
Vázquez, M.
Ozafrán, M.
Durán, H.
Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
topic_facet γH2AX
CHO10B2
DNA double strand break
DNA-PKcs
H2AX foci size
Ionising radiation
irs-20
LET
Linear energy transfer
histone gamma h2ax
histone H2AX
lithium
unclassified drug
animal cell
article
controlled study
DNA damage
double stranded DNA break
gamma irradiation
immunofluorescence
ionizing radiation
linear energy transfer
nonhuman
protein phosphorylation
radiation dose fractionation
radiosensitivity
description The aim of this study was to evaluate the DNA damage induced by low and high linear energy transfer radiation in two related cell lines with different radiation sensitivity, CHO10B2 and irs-20 cells (defective in DNAPKcs). Double-strand breaks were assessed by the detection of phosphorylated histone H2AX foci (γH2AX). The number of foci increased as a function of dose for both cell lines and types of radiation. However, irs-20 cells showed a higher number of foci than the parental CHO10B2. An increase in the foci size was observed for both cell lines after lithium irradiation. This could be attributed to clusters of DNA damage. Moreover, the number of lithiuminduced larger foci per nuclei increased with dose and fitted with the expected number of hits per nucleus. Concluding, γH2AX foci size provides a potential tool to characterise the intrinsic radiosensitivity of cells related to DNA damage and to compare the effects of different quality radiations. Copyright © 2010 Inderscience Enterprises Ltd.
format JOUR
author Bracalente, C.
Ibañez, I.L.
Molinari, B.
Palmieri, M.A.
Maglioco, A.
Policastro, L.
Kreiner, A.J.
Burlón, A.
Valda, A.
Davidson, J.
Davidson, M.
Vázquez, M.
Ozafrán, M.
Durán, H.
author_facet Bracalente, C.
Ibañez, I.L.
Molinari, B.
Palmieri, M.A.
Maglioco, A.
Policastro, L.
Kreiner, A.J.
Burlón, A.
Valda, A.
Davidson, J.
Davidson, M.
Vázquez, M.
Ozafrán, M.
Durán, H.
author_sort Bracalente, C.
title Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
title_short Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
title_full Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
title_fullStr Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
title_full_unstemmed Assessment of γh2AX nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
title_sort assessment of γh2ax nuclear foci number and size in normal and repair-deficient cells irradiated with low and high linear energy transfer radiation
url http://hdl.handle.net/20.500.12110/paper_14776545_v7_n5_p393_Bracalente
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