Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring...

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Autores principales:	Liras, M., Simoncelli, S., Rivas-Aravena, A., García, O., Scaiano, J.C., Alarcon, E.I., Aspée, A.
Formato:	JOUR
Materias:	Amides Cell culture Cytology Fibroblasts Fluorescence Fluorescence microscopy Cellular redox Fluorescence intensities Human fibroblast Nitroxides Probes 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene amide boron derivative fluorescent dye hydrogen peroxide nitrogen oxide cell culture chemistry fibroblast fluorescence microscopy human metabolism oxidation reduction reaction synthesis Boron Compounds Cells, Cultured Fluorescent Dyes Humans Hydrogen Peroxide Microscopy, Fluorescence Nitrogen Oxides Oxidation-Reduction
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_14770520_v14_n17_p4023_Liras
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_14770520_v14_n17_p4023_Liras
record_format	dspace
spelling	todo:paper_14770520_v14_n17_p4023_Liras2023-10-03T16:19:03Z Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy Liras, M. Simoncelli, S. Rivas-Aravena, A. García, O. Scaiano, J.C. Alarcon, E.I. Aspée, A. Amides Cell culture Cytology Fibroblasts Fluorescence Fluorescence microscopy Cellular redox Fluorescence intensities Human fibroblast Nitroxides Probes 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene amide boron derivative fluorescent dye hydrogen peroxide nitrogen oxide cell culture chemistry fibroblast fluorescence microscopy human metabolism oxidation reduction reaction synthesis Amides Boron Compounds Cells, Cultured Fibroblasts Fluorescent Dyes Humans Hydrogen Peroxide Microscopy, Fluorescence Nitrogen Oxides Oxidation-Reduction A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring nitroxide depletion by hydrogen peroxide; eluding incorrect interpretation if only fluorescence intensity is considered. © 2016 The Royal Society of Chemistry. Fil:Simoncelli, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14770520_v14_n17_p4023_Liras
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Amides Cell culture Cytology Fibroblasts Fluorescence Fluorescence microscopy Cellular redox Fluorescence intensities Human fibroblast Nitroxides Probes 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene amide boron derivative fluorescent dye hydrogen peroxide nitrogen oxide cell culture chemistry fibroblast fluorescence microscopy human metabolism oxidation reduction reaction synthesis Amides Boron Compounds Cells, Cultured Fibroblasts Fluorescent Dyes Humans Hydrogen Peroxide Microscopy, Fluorescence Nitrogen Oxides Oxidation-Reduction
spellingShingle	Amides Cell culture Cytology Fibroblasts Fluorescence Fluorescence microscopy Cellular redox Fluorescence intensities Human fibroblast Nitroxides Probes 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene amide boron derivative fluorescent dye hydrogen peroxide nitrogen oxide cell culture chemistry fibroblast fluorescence microscopy human metabolism oxidation reduction reaction synthesis Amides Boron Compounds Cells, Cultured Fibroblasts Fluorescent Dyes Humans Hydrogen Peroxide Microscopy, Fluorescence Nitrogen Oxides Oxidation-Reduction Liras, M. Simoncelli, S. Rivas-Aravena, A. García, O. Scaiano, J.C. Alarcon, E.I. Aspée, A. Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy
topic_facet	Amides Cell culture Cytology Fibroblasts Fluorescence Fluorescence microscopy Cellular redox Fluorescence intensities Human fibroblast Nitroxides Probes 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene amide boron derivative fluorescent dye hydrogen peroxide nitrogen oxide cell culture chemistry fibroblast fluorescence microscopy human metabolism oxidation reduction reaction synthesis Amides Boron Compounds Cells, Cultured Fibroblasts Fluorescent Dyes Humans Hydrogen Peroxide Microscopy, Fluorescence Nitrogen Oxides Oxidation-Reduction
description	A novel synthesized nitroxide amide-BODIPY prefluorescent probe was used to study cellular redox balance that modulates nitroxide/hydroxylamine ratio in cultured human fibroblasts. FLIM quantitatively differentiated between nitroxide states of the cytoplasm-localized probe imaged by TIRF, monitoring nitroxide depletion by hydrogen peroxide; eluding incorrect interpretation if only fluorescence intensity is considered. © 2016 The Royal Society of Chemistry.
format	JOUR
author	Liras, M. Simoncelli, S. Rivas-Aravena, A. García, O. Scaiano, J.C. Alarcon, E.I. Aspée, A.
author_facet	Liras, M. Simoncelli, S. Rivas-Aravena, A. García, O. Scaiano, J.C. Alarcon, E.I. Aspée, A.
author_sort	Liras, M.
title	Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy
title_short	Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy
title_full	Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy
title_fullStr	Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy
title_full_unstemmed	Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy
title_sort	nitroxide amide-bodipy probe behavior in fibroblasts analyzed by advanced fluorescence microscopy
url	http://hdl.handle.net/20.500.12110/paper_14770520_v14_n17_p4023_Liras
work_keys_str_mv	AT lirasm nitroxideamidebodipyprobebehaviorinfibroblastsanalyzedbyadvancedfluorescencemicroscopy AT simoncellis nitroxideamidebodipyprobebehaviorinfibroblastsanalyzedbyadvancedfluorescencemicroscopy AT rivasaravenaa nitroxideamidebodipyprobebehaviorinfibroblastsanalyzedbyadvancedfluorescencemicroscopy AT garciao nitroxideamidebodipyprobebehaviorinfibroblastsanalyzedbyadvancedfluorescencemicroscopy AT scaianojc nitroxideamidebodipyprobebehaviorinfibroblastsanalyzedbyadvancedfluorescencemicroscopy AT alarconei nitroxideamidebodipyprobebehaviorinfibroblastsanalyzedbyadvancedfluorescencemicroscopy AT aspeea nitroxideamidebodipyprobebehaviorinfibroblastsanalyzedbyadvancedfluorescencemicroscopy
_version_	1782025038800617472

Nitroxide amide-BODIPY probe behavior in fibroblasts analyzed by advanced fluorescence microscopy

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