A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples

Chlorophyll-a contained in the peel of Granny Smith apples emits fluorescence upon excitation with blue light. The observed emission, collected by an external detector and corrected by its spectral response, is still distorted by light reabsorption processes taking place in the fruit skin and differ...

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Autores principales:	Ramos, M.E., Lagorio, M.G.
Formato:	JOUR
Materias:	chlorophyll apple article blue light chloroplast correlation analysis fluorescence light absorption measurement model nonhuman photosynthesis priority journal spectroscopy Absorption Chlorophyll Fluorescence Light Malus Models, Chemical Spectrometry, Fluorescence Malus x domestica
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_1474905X_v5_n5_p508_Ramos
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_1474905X_v5_n5_p508_Ramos
record_format	dspace
spelling	todo:paper_1474905X_v5_n5_p508_Ramos2023-10-03T16:18:29Z A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples Ramos, M.E. Lagorio, M.G. chlorophyll apple article blue light chloroplast correlation analysis fluorescence light absorption measurement model nonhuman photosynthesis priority journal spectroscopy Absorption Chlorophyll Fluorescence Light Malus Models, Chemical Spectrometry, Fluorescence Malus x domestica Chlorophyll-a contained in the peel of Granny Smith apples emits fluorescence upon excitation with blue light. The observed emission, collected by an external detector and corrected by its spectral response, is still distorted by light reabsorption processes taking place in the fruit skin and differs appreciably from the true spectral distribution of fluorescence emerging from chlorophyll molecules in the biological tissue. Reabsorption processes particularly affect the ratio of fluorescence intensities at 680 nm and at 730 nm. A model to obtain the correct spectral distribution of the emission, from the experimental fluorescence recorded at a fluorometer detector and corrected for the detector spectral sensitivity, is developed in the present work. Measurements of the whole fruit reflectance, the peel transmittance and the flesh reflectance allow the calculation of the reabsorption-corrected spectra. The model is validated by comparing the corrected emission spectra with that obtained for a thin layer of apple-peel-chloroplasts, where no reabsorption takes place. It is recommended to correct distortions in emission spectra of intact fruits due to light reabsorption effects whenever a correlation between the physiological state of the fruit and its fluorescence spectra is investigated. © The Royal Society of Chemistry and Owner Societies 2006. Fil:Ramos, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lagorio, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_1474905X_v5_n5_p508_Ramos
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	chlorophyll apple article blue light chloroplast correlation analysis fluorescence light absorption measurement model nonhuman photosynthesis priority journal spectroscopy Absorption Chlorophyll Fluorescence Light Malus Models, Chemical Spectrometry, Fluorescence Malus x domestica
spellingShingle	chlorophyll apple article blue light chloroplast correlation analysis fluorescence light absorption measurement model nonhuman photosynthesis priority journal spectroscopy Absorption Chlorophyll Fluorescence Light Malus Models, Chemical Spectrometry, Fluorescence Malus x domestica Ramos, M.E. Lagorio, M.G. A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples
topic_facet	chlorophyll apple article blue light chloroplast correlation analysis fluorescence light absorption measurement model nonhuman photosynthesis priority journal spectroscopy Absorption Chlorophyll Fluorescence Light Malus Models, Chemical Spectrometry, Fluorescence Malus x domestica
description	Chlorophyll-a contained in the peel of Granny Smith apples emits fluorescence upon excitation with blue light. The observed emission, collected by an external detector and corrected by its spectral response, is still distorted by light reabsorption processes taking place in the fruit skin and differs appreciably from the true spectral distribution of fluorescence emerging from chlorophyll molecules in the biological tissue. Reabsorption processes particularly affect the ratio of fluorescence intensities at 680 nm and at 730 nm. A model to obtain the correct spectral distribution of the emission, from the experimental fluorescence recorded at a fluorometer detector and corrected for the detector spectral sensitivity, is developed in the present work. Measurements of the whole fruit reflectance, the peel transmittance and the flesh reflectance allow the calculation of the reabsorption-corrected spectra. The model is validated by comparing the corrected emission spectra with that obtained for a thin layer of apple-peel-chloroplasts, where no reabsorption takes place. It is recommended to correct distortions in emission spectra of intact fruits due to light reabsorption effects whenever a correlation between the physiological state of the fruit and its fluorescence spectra is investigated. © The Royal Society of Chemistry and Owner Societies 2006.
format	JOUR
author	Ramos, M.E. Lagorio, M.G.
author_facet	Ramos, M.E. Lagorio, M.G.
author_sort	Ramos, M.E.
title	A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples
title_short	A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples
title_full	A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples
title_fullStr	A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples
title_full_unstemmed	A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples
title_sort	model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples
url	http://hdl.handle.net/20.500.12110/paper_1474905X_v5_n5_p508_Ramos
work_keys_str_mv	AT ramosme amodelconsideringlightreabsorptionprocessestocorrectinvivochlorophyllfluorescencespectrainapples AT lagoriomg amodelconsideringlightreabsorptionprocessestocorrectinvivochlorophyllfluorescencespectrainapples AT ramosme modelconsideringlightreabsorptionprocessestocorrectinvivochlorophyllfluorescencespectrainapples AT lagoriomg modelconsideringlightreabsorptionprocessestocorrectinvivochlorophyllfluorescencespectrainapples
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A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples

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