A comparative study of the photophysical and phototoxic properties of octakis(decyloxy)phthalocyaninato zinc(II), incorporated in a hydrophilic polymer, in liposomes and in non-ionic micelles

Factors such as charge, aggregation and lipophilicity influence photosensitiser localisation. The lipophilic octasubstituted sensitiser 2,3,9,10,16,17,23,24-octakis(decyloxy)phthalocyaninato zinc(II) was incorporated into liposomes of dimyristoyl-L-α-phosphatidylcholine (DMPC), non-ionic micelles of...

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Detalles Bibliográficos
Autores principales: Rodriguez, M.E., Morán, F., Bonansea, A., Monetti, M., Fernández, D.A., Strassert, C.A., Rivarola, V., Awruch, J., Dicelio, L.E.
Formato: JOUR
Materias:
2,3,9,10,16,17,23,24 octakis(decyloxy)phthalocyaninato zinc
dimyristoylphosphatidylcholine
liposome
photosensitizing agent
polymer
polysorbate 80
singlet oxygen
solutol hs 15
unclassified drug
zinc derivative
organometallic compound
apoptosis
article
cell death
cell viability
controlled study
drug mechanism
drug structure
HEp 2 cell
human
human cell
irradiation
lipophilicity
micelle
photodynamic therapy
phototoxicity
priority journal
biology
cell line
cell survival
chemistry
comparative study
drug effect
necrosis
photochemistry
photochemotherapy
Apoptosis
Cell Line
Cell Survival
Dimyristoylphosphatidylcholine
Humans
Liposomes
Micelles
Necrosis
Organometallic Compounds
Photobiology
Photochemistry
Photochemotherapy
Photosensitizing Agents
Polymers
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_1474905X_v2_n10_p988_Rodriguez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Factors such as charge, aggregation and lipophilicity influence photosensitiser localisation. The lipophilic octasubstituted sensitiser 2,3,9,10,16,17,23,24-octakis(decyloxy)phthalocyaninato zinc(II) was incorporated into liposomes of dimyristoyl-L-α-phosphatidylcholine (DMPC), non-ionic micelles of Tween 80 and the hydrosoluble polymer Solutol® HS 15 in order to investigate how these different environments affect the photophysical properties and phototoxicity of the photosensitiser. Fluorescence quantum yields and singlet molecular oxygen generation are enhanced in the presence of Solutol® HS 15. Phototoxicities were calculated by employing a concentration of 10-7 M of the dye against the Hcp-2 cell line, which showed a viability of 53 and 30% in DMPC and Solutol® HS 15, respectively. After 24 h of photodynamic therapy with 15 min irradiation, apoptotic and necrotic cells were observed. © The Royal Society of Chemistry and Owner Societies 2003.

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