Differential representation of sunflower ESTs in enriched organ-specific cDNA libraries in a small scale sequencing project

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Background: Subtractive hybridization methods are valuable tools for identifying differentially regulated genes in a given tissue avoiding redundant sequencing of clones representing the same expressed genes, maximizing detection of low abundant transcripts and thus, affecting the efficiency and cos...

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Detalles Bibliográficos
Autores principales: Fernández, P., Paniego, N., Lew, S., Hopp, H.E., Heinz, R.A.
Formato: JOUR
Materias:
EST
Helianthus annuus
Organ-specific transcripts
SSH
Sunflower
complementary DNA
RNA
agronomic trait
article
breeding
controlled study
cost effectiveness analysis
developmental stage
DNA library
DNA sequence
expressed sequence tag
flower
gene expression
gene function
gene identification
gene isolation
gene sequence
genetic association
genetic regulation
genetic trait
genetic transcription
marker gene
molecular cloning
nonhuman
nucleotide sequence
plant genetics
plant leaf
plant root
plant stem
prediction
redundancy analysis
regulator gene
sequence analysis
sequence database
sequence homology
subtractive hybridization
sunflower
tissue specificity
chemistry
comparative study
gene expression profiling
gene expression regulation
gene library
genetics
growth, development and aging
methodology
nucleic acid hybridization
reproducibility
sensitivity and specificity
Helianthus
DNA, Complementary
Expressed Sequence Tags
Flowers
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Gene Library
Nucleic Acid Hybridization
Plant Leaves
Plant Roots
Plant Stems
Reproducibility of Results
Sensitivity and Specificity
Sequence Analysis, DNA
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_14712164_v4_n_p_Fernandez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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