Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats
Leptin exerts both stimulatory and inhibitory effects on the ovulatory process. In this study, we investigated whether these opposite effects involve changes in the oxidative status in response to different levels of leptin. To this end, we performed both in vivo and in vitro assays using ovaries of...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_14701626_v149_n4_p357_Bilbao |
| Aporte de: |
| id |
todo:paper_14701626_v149_n4_p357_Bilbao |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_14701626_v149_n4_p357_Bilbao2023-10-03T16:17:37Z Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats Bilbao, M.G. Di Yorio, M.P. Galarza, R.A. Varone, C.L. Faletti, A.G. caspase 3 catalase glutathione gonadotropin leptin protein Bax protein bcl 2 reactive oxygen metabolite superoxide dismutase caspase 3 catalase glutathione leptin superoxide dismutase animal cell animal experiment animal tissue apoptosis Article controlled study explant female immaturity in vitro study in vivo study investigative procedures lipid peroxidation nonhuman ovary function ovulation oxidative stress priority journal protein expression rat Western blotting animal drug effects metabolism ovary ovulation oxidation reduction reaction Sprague Dawley rat Rattus Animals Blotting, Western Caspase 3 Catalase Female Glutathione Leptin Lipid Peroxidation Ovary Ovulation Oxidation-Reduction Oxidative Stress Rats Rats, Sprague-Dawley Superoxide Dismutase Leptin exerts both stimulatory and inhibitory effects on the ovulatory process. In this study, we investigated whether these opposite effects involve changes in the oxidative status in response to different levels of leptin. To this end, we performed both in vivo and in vitro assays using ovaries of immature rats primed with gonadotropins to induce ovulation. Superoxide dismutase (SOD) and catalase (CAT) activity, lipid peroxidation, glutathione (GSH) content, and reactive oxygen species (ROS) were studied as oxidative damage-related parameters. The expression of BCL2, BAX, and caspase 3 were measured by western blot as apoptosis-related biomarkers. The acute treatment with leptin, which inhibits ovulation, decreased SOD activity and increased active caspase 3 expression. No differences were found in CATactivity, lipid peroxidation, or total GSH. In contrast, the daily administration of leptin, which induces ovulation, decreased GSH content, ROS levels, and Bax and active caspase 3 expression, but caused no changes in other parameters. In addition, the daily administration of leptin induced follicular growth, measured by the number of antral follicles in ovarian sections. Using ovarian explant cultures, we found increased BCL2 expression and decreased SOD activity at low and high concentrations of leptin respectively. Thus, leptin can modulate the oxidative status of the ovarian tissue, during the ovulatory process, by acting on different targets according to its circulating levels. At low concentration, leptin seems to play a protective role against the oxidative stress, whereas at high concentrations, this protein seems to be involved in cell death. © 2015 Society for Reproduction and Fertility. Fil:Bilbao, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Faletti, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14701626_v149_n4_p357_Bilbao |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
caspase 3 catalase glutathione gonadotropin leptin protein Bax protein bcl 2 reactive oxygen metabolite superoxide dismutase caspase 3 catalase glutathione leptin superoxide dismutase animal cell animal experiment animal tissue apoptosis Article controlled study explant female immaturity in vitro study in vivo study investigative procedures lipid peroxidation nonhuman ovary function ovulation oxidative stress priority journal protein expression rat Western blotting animal drug effects metabolism ovary ovulation oxidation reduction reaction Sprague Dawley rat Rattus Animals Blotting, Western Caspase 3 Catalase Female Glutathione Leptin Lipid Peroxidation Ovary Ovulation Oxidation-Reduction Oxidative Stress Rats Rats, Sprague-Dawley Superoxide Dismutase |
| spellingShingle |
caspase 3 catalase glutathione gonadotropin leptin protein Bax protein bcl 2 reactive oxygen metabolite superoxide dismutase caspase 3 catalase glutathione leptin superoxide dismutase animal cell animal experiment animal tissue apoptosis Article controlled study explant female immaturity in vitro study in vivo study investigative procedures lipid peroxidation nonhuman ovary function ovulation oxidative stress priority journal protein expression rat Western blotting animal drug effects metabolism ovary ovulation oxidation reduction reaction Sprague Dawley rat Rattus Animals Blotting, Western Caspase 3 Catalase Female Glutathione Leptin Lipid Peroxidation Ovary Ovulation Oxidation-Reduction Oxidative Stress Rats Rats, Sprague-Dawley Superoxide Dismutase Bilbao, M.G. Di Yorio, M.P. Galarza, R.A. Varone, C.L. Faletti, A.G. Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats |
| topic_facet |
caspase 3 catalase glutathione gonadotropin leptin protein Bax protein bcl 2 reactive oxygen metabolite superoxide dismutase caspase 3 catalase glutathione leptin superoxide dismutase animal cell animal experiment animal tissue apoptosis Article controlled study explant female immaturity in vitro study in vivo study investigative procedures lipid peroxidation nonhuman ovary function ovulation oxidative stress priority journal protein expression rat Western blotting animal drug effects metabolism ovary ovulation oxidation reduction reaction Sprague Dawley rat Rattus Animals Blotting, Western Caspase 3 Catalase Female Glutathione Leptin Lipid Peroxidation Ovary Ovulation Oxidation-Reduction Oxidative Stress Rats Rats, Sprague-Dawley Superoxide Dismutase |
| description |
Leptin exerts both stimulatory and inhibitory effects on the ovulatory process. In this study, we investigated whether these opposite effects involve changes in the oxidative status in response to different levels of leptin. To this end, we performed both in vivo and in vitro assays using ovaries of immature rats primed with gonadotropins to induce ovulation. Superoxide dismutase (SOD) and catalase (CAT) activity, lipid peroxidation, glutathione (GSH) content, and reactive oxygen species (ROS) were studied as oxidative damage-related parameters. The expression of BCL2, BAX, and caspase 3 were measured by western blot as apoptosis-related biomarkers. The acute treatment with leptin, which inhibits ovulation, decreased SOD activity and increased active caspase 3 expression. No differences were found in CATactivity, lipid peroxidation, or total GSH. In contrast, the daily administration of leptin, which induces ovulation, decreased GSH content, ROS levels, and Bax and active caspase 3 expression, but caused no changes in other parameters. In addition, the daily administration of leptin induced follicular growth, measured by the number of antral follicles in ovarian sections. Using ovarian explant cultures, we found increased BCL2 expression and decreased SOD activity at low and high concentrations of leptin respectively. Thus, leptin can modulate the oxidative status of the ovarian tissue, during the ovulatory process, by acting on different targets according to its circulating levels. At low concentration, leptin seems to play a protective role against the oxidative stress, whereas at high concentrations, this protein seems to be involved in cell death. © 2015 Society for Reproduction and Fertility. |
| format |
JOUR |
| author |
Bilbao, M.G. Di Yorio, M.P. Galarza, R.A. Varone, C.L. Faletti, A.G. |
| author_facet |
Bilbao, M.G. Di Yorio, M.P. Galarza, R.A. Varone, C.L. Faletti, A.G. |
| author_sort |
Bilbao, M.G. |
| title |
Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats |
| title_short |
Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats |
| title_full |
Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats |
| title_fullStr |
Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats |
| title_full_unstemmed |
Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats |
| title_sort |
regulation of the ovarian oxidative status by leptin during the ovulatory process in rats |
| url |
http://hdl.handle.net/20.500.12110/paper_14701626_v149_n4_p357_Bilbao |
| work_keys_str_mv |
AT bilbaomg regulationoftheovarianoxidativestatusbyleptinduringtheovulatoryprocessinrats AT diyoriomp regulationoftheovarianoxidativestatusbyleptinduringtheovulatoryprocessinrats AT galarzara regulationoftheovarianoxidativestatusbyleptinduringtheovulatoryprocessinrats AT varonecl regulationoftheovarianoxidativestatusbyleptinduringtheovulatoryprocessinrats AT falettiag regulationoftheovarianoxidativestatusbyleptinduringtheovulatoryprocessinrats |
| _version_ |
1782023938273968128 |