Potential immunomodulatory role of VIP in the implantation sites of prediabetic nonobese diabetic mice
Among several factors known to modulate embryo implantation and survival, uterine quiescence and neovascularization, maternal immunotolerance through the Th1/Th2 cytokine balance towards a Th2 profile, local regulatory T-cell (Treg) activation, and high levels of progesterone were assigned a promine...
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todo:paper_14701626_v138_n4_p733_Roca2023-10-03T16:17:36Z Potential immunomodulatory role of VIP in the implantation sites of prediabetic nonobese diabetic mice Roca, V. Calafat, M. Larocca, L. Ramhorst, R. Farina, M. Franchi, A.M. Pérez Leirós, C. progesterone vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 alloimmunity animal experiment animal model animal tissue article birth cell activation controlled study embryo resorption female immunomodulation implantation lifespan litter size male mouse nonhuman nonobese diabetic mouse priority journal progesterone blood level protein expression protein function regulatory T lymphocyte Sjoegren syndrome spleen cell Th1 cell Animals Diabetes, Gestational Embryo Implantation Embryo Loss Female Immunologic Factors Litter Size Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred NOD Prediabetic State Pregnancy Receptors, Vasoactive Intestinal Peptide, Type II Receptors, Vasoactive Intestinal Polypeptide, Type I T-Lymphocytes, Regulatory Vasoactive Intestinal Peptide Mus Among several factors known to modulate embryo implantation and survival, uterine quiescence and neovascularization, maternal immunotolerance through the Th1/Th2 cytokine balance towards a Th2 profile, local regulatory T-cell (Treg) activation, and high levels of progesterone were assigned a prominent role. Vasoactive intestinal peptide (VIP) is a neuroimmunopeptide that has anti-inflammatory effects, promotes Th2 cytokines and CD4 +CD25 +FOXP3 + Treg activation, and stimulates exocrine secretion, smooth muscle relaxation, and vasodilatation favoring uterus quiescence. The goal of the present work was to explore the participation of VIP in the implantation sites of normal and pregnant prediabetic nonobese diabetic (NOD) females, a mouse strain that spontaneously develops an autoimmune exocrinopathy similar to Sjögren's syndrome. Our results indicate a reduction in litter size from the third parturition onwards in the NOD female lifespan with increased resorption rates. Progesterone systemic levels were significantly decreased in pregnant NOD mice compared with BALB/c mice, although the allogeneic response to progesterone by spleen cells was not impaired. VIP receptors, Vipr1 and Vipr2 (Vpac1 and Vpac2), were expressed at the implantation sites and VIP induced leukemia inhibitory factor (LIF) and Treg marker expression in both strains; however, a reduced Vip expression was found in NOD implantation sites. We conclude that the reduced birth rate at 16-week-old NOD mice with a Th1 systemic cytokine profile involves resorption processes with a lower expression of VIP at the sites of implantation, which acts as a local inducer of pro-implantatory LIF and Treg activation. © 2009 Society for Reproduction and Fertility. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14701626_v138_n4_p733_Roca |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
progesterone vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 alloimmunity animal experiment animal model animal tissue article birth cell activation controlled study embryo resorption female immunomodulation implantation lifespan litter size male mouse nonhuman nonobese diabetic mouse priority journal progesterone blood level protein expression protein function regulatory T lymphocyte Sjoegren syndrome spleen cell Th1 cell Animals Diabetes, Gestational Embryo Implantation Embryo Loss Female Immunologic Factors Litter Size Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred NOD Prediabetic State Pregnancy Receptors, Vasoactive Intestinal Peptide, Type II Receptors, Vasoactive Intestinal Polypeptide, Type I T-Lymphocytes, Regulatory Vasoactive Intestinal Peptide Mus |
| spellingShingle |
progesterone vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 alloimmunity animal experiment animal model animal tissue article birth cell activation controlled study embryo resorption female immunomodulation implantation lifespan litter size male mouse nonhuman nonobese diabetic mouse priority journal progesterone blood level protein expression protein function regulatory T lymphocyte Sjoegren syndrome spleen cell Th1 cell Animals Diabetes, Gestational Embryo Implantation Embryo Loss Female Immunologic Factors Litter Size Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred NOD Prediabetic State Pregnancy Receptors, Vasoactive Intestinal Peptide, Type II Receptors, Vasoactive Intestinal Polypeptide, Type I T-Lymphocytes, Regulatory Vasoactive Intestinal Peptide Mus Roca, V. Calafat, M. Larocca, L. Ramhorst, R. Farina, M. Franchi, A.M. Pérez Leirós, C. Potential immunomodulatory role of VIP in the implantation sites of prediabetic nonobese diabetic mice |
| topic_facet |
progesterone vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 alloimmunity animal experiment animal model animal tissue article birth cell activation controlled study embryo resorption female immunomodulation implantation lifespan litter size male mouse nonhuman nonobese diabetic mouse priority journal progesterone blood level protein expression protein function regulatory T lymphocyte Sjoegren syndrome spleen cell Th1 cell Animals Diabetes, Gestational Embryo Implantation Embryo Loss Female Immunologic Factors Litter Size Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred NOD Prediabetic State Pregnancy Receptors, Vasoactive Intestinal Peptide, Type II Receptors, Vasoactive Intestinal Polypeptide, Type I T-Lymphocytes, Regulatory Vasoactive Intestinal Peptide Mus |
| description |
Among several factors known to modulate embryo implantation and survival, uterine quiescence and neovascularization, maternal immunotolerance through the Th1/Th2 cytokine balance towards a Th2 profile, local regulatory T-cell (Treg) activation, and high levels of progesterone were assigned a prominent role. Vasoactive intestinal peptide (VIP) is a neuroimmunopeptide that has anti-inflammatory effects, promotes Th2 cytokines and CD4 +CD25 +FOXP3 + Treg activation, and stimulates exocrine secretion, smooth muscle relaxation, and vasodilatation favoring uterus quiescence. The goal of the present work was to explore the participation of VIP in the implantation sites of normal and pregnant prediabetic nonobese diabetic (NOD) females, a mouse strain that spontaneously develops an autoimmune exocrinopathy similar to Sjögren's syndrome. Our results indicate a reduction in litter size from the third parturition onwards in the NOD female lifespan with increased resorption rates. Progesterone systemic levels were significantly decreased in pregnant NOD mice compared with BALB/c mice, although the allogeneic response to progesterone by spleen cells was not impaired. VIP receptors, Vipr1 and Vipr2 (Vpac1 and Vpac2), were expressed at the implantation sites and VIP induced leukemia inhibitory factor (LIF) and Treg marker expression in both strains; however, a reduced Vip expression was found in NOD implantation sites. We conclude that the reduced birth rate at 16-week-old NOD mice with a Th1 systemic cytokine profile involves resorption processes with a lower expression of VIP at the sites of implantation, which acts as a local inducer of pro-implantatory LIF and Treg activation. © 2009 Society for Reproduction and Fertility. |
| format |
JOUR |
| author |
Roca, V. Calafat, M. Larocca, L. Ramhorst, R. Farina, M. Franchi, A.M. Pérez Leirós, C. |
| author_facet |
Roca, V. Calafat, M. Larocca, L. Ramhorst, R. Farina, M. Franchi, A.M. Pérez Leirós, C. |
| author_sort |
Roca, V. |
| title |
Potential immunomodulatory role of VIP in the implantation sites of prediabetic nonobese diabetic mice |
| title_short |
Potential immunomodulatory role of VIP in the implantation sites of prediabetic nonobese diabetic mice |
| title_full |
Potential immunomodulatory role of VIP in the implantation sites of prediabetic nonobese diabetic mice |
| title_fullStr |
Potential immunomodulatory role of VIP in the implantation sites of prediabetic nonobese diabetic mice |
| title_full_unstemmed |
Potential immunomodulatory role of VIP in the implantation sites of prediabetic nonobese diabetic mice |
| title_sort |
potential immunomodulatory role of vip in the implantation sites of prediabetic nonobese diabetic mice |
| url |
http://hdl.handle.net/20.500.12110/paper_14701626_v138_n4_p733_Roca |
| work_keys_str_mv |
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