Spatiotemporal analysis of the protein expression of angiogenic factors and their related receptors during folliculogenesis in rats with and without hormonal treatment
This study investigated the protein expression and cellular localization of ANGPT1, ANGPT2, and their receptor TEK, as well as vascular endothelial growth factor A (VEGFA) and its receptor KDR (VEGFR2) during folliculogenesis. To obtain follicles at different stages for immunochemistry and western a...
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todo:paper_14701626_v137_n2_p309_Abramovich2023-10-03T16:17:35Z Spatiotemporal analysis of the protein expression of angiogenic factors and their related receptors during folliculogenesis in rats with and without hormonal treatment Abramovich, D. Celin, A.R. Hernandez, F. Tesone, M. Parborell, F. angiopoietin 1 angiopoietin 2 chorionic gonadotropin tyrosine kinase receptor vasculotropin A vasculotropin receptor 2 angiogenic factor angiopoietin 1 angiopoietin 2 angiopoietin receptor diethylstilbestrol seric gonadotropin vascular endothelial growth factor A, rat vasculotropin A vasculotropin receptor 2 animal cell animal experiment animal tissue article controlled study developmental stage female granulosa cell immunochemistry nonhuman ovary follicle development ovary follicle maturation priority journal protein expression protein localization rat theca cell Western blotting animal comparative study drug effect gene expression immunohistochemistry metabolism methodology ovary follicle physiology Sprague Dawley rat Equidae Rattus Angiogenesis Inducing Agents Angiopoietin-1 Angiopoietin-2 Animals Blotting, Western Diethylstilbestrol Female Gene Expression Gonadotropins, Equine Immunohistochemistry Ovarian Follicle Rats Rats, Sprague-Dawley Receptor, TIE-2 Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 This study investigated the protein expression and cellular localization of ANGPT1, ANGPT2, and their receptor TEK, as well as vascular endothelial growth factor A (VEGFA) and its receptor KDR (VEGFR2) during folliculogenesis. To obtain follicles at different stages for immunochemistry and western analyses, we used prepubertal untreated, diethylstilbestrol- and equine chorionic gonadotropin-treated rats. To confirm that these hormonal treatments reflect physiological change, we used non-treated adult rats. No expression of ANGPT1 was observed in granulosa cells (Gc) from immature hormone-treated and non-treated rats at any follicular stage. By contrast, ANGPT1 expression in theca cells (Tc) increased with follicular maturation. ANGPT2 protein was either absent or weakly expressed in Gc at all follicular stages. In Tc, minimal expression of ANGPT2 protein was detected in the preantral follicle (PF), whereas it was stronger in the early antral follicle (EAF) and preovulatory follicle (POF). TEK staining was absent in Gc but was intense in Tc at every follicular stage. Staining for VEGFA was either absent or weakly present in Gc and Tc in PF and EAF, although in POF it was stronger in Gc and Tc. Staining for KDR was absent in Gc and very low in Tc from PF. Gc and Tc of EAF showed positive staining for KDR and in POF the staining was stronger. These results were confirmed by western immunoblot. A similar pattern of expression of these proteins was observed in cycling rats. In conclusion, we observed that the protein expression of ANGPT1, ANGPT2, VEGFA and their receptors increased during follicular development in rats. © 2009 Society for Reproduction and Fertility. Fil:Abramovich, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Parborell, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14701626_v137_n2_p309_Abramovich |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
angiopoietin 1 angiopoietin 2 chorionic gonadotropin tyrosine kinase receptor vasculotropin A vasculotropin receptor 2 angiogenic factor angiopoietin 1 angiopoietin 2 angiopoietin receptor diethylstilbestrol seric gonadotropin vascular endothelial growth factor A, rat vasculotropin A vasculotropin receptor 2 animal cell animal experiment animal tissue article controlled study developmental stage female granulosa cell immunochemistry nonhuman ovary follicle development ovary follicle maturation priority journal protein expression protein localization rat theca cell Western blotting animal comparative study drug effect gene expression immunohistochemistry metabolism methodology ovary follicle physiology Sprague Dawley rat Equidae Rattus Angiogenesis Inducing Agents Angiopoietin-1 Angiopoietin-2 Animals Blotting, Western Diethylstilbestrol Female Gene Expression Gonadotropins, Equine Immunohistochemistry Ovarian Follicle Rats Rats, Sprague-Dawley Receptor, TIE-2 Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 |
| spellingShingle |
angiopoietin 1 angiopoietin 2 chorionic gonadotropin tyrosine kinase receptor vasculotropin A vasculotropin receptor 2 angiogenic factor angiopoietin 1 angiopoietin 2 angiopoietin receptor diethylstilbestrol seric gonadotropin vascular endothelial growth factor A, rat vasculotropin A vasculotropin receptor 2 animal cell animal experiment animal tissue article controlled study developmental stage female granulosa cell immunochemistry nonhuman ovary follicle development ovary follicle maturation priority journal protein expression protein localization rat theca cell Western blotting animal comparative study drug effect gene expression immunohistochemistry metabolism methodology ovary follicle physiology Sprague Dawley rat Equidae Rattus Angiogenesis Inducing Agents Angiopoietin-1 Angiopoietin-2 Animals Blotting, Western Diethylstilbestrol Female Gene Expression Gonadotropins, Equine Immunohistochemistry Ovarian Follicle Rats Rats, Sprague-Dawley Receptor, TIE-2 Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 Abramovich, D. Celin, A.R. Hernandez, F. Tesone, M. Parborell, F. Spatiotemporal analysis of the protein expression of angiogenic factors and their related receptors during folliculogenesis in rats with and without hormonal treatment |
| topic_facet |
angiopoietin 1 angiopoietin 2 chorionic gonadotropin tyrosine kinase receptor vasculotropin A vasculotropin receptor 2 angiogenic factor angiopoietin 1 angiopoietin 2 angiopoietin receptor diethylstilbestrol seric gonadotropin vascular endothelial growth factor A, rat vasculotropin A vasculotropin receptor 2 animal cell animal experiment animal tissue article controlled study developmental stage female granulosa cell immunochemistry nonhuman ovary follicle development ovary follicle maturation priority journal protein expression protein localization rat theca cell Western blotting animal comparative study drug effect gene expression immunohistochemistry metabolism methodology ovary follicle physiology Sprague Dawley rat Equidae Rattus Angiogenesis Inducing Agents Angiopoietin-1 Angiopoietin-2 Animals Blotting, Western Diethylstilbestrol Female Gene Expression Gonadotropins, Equine Immunohistochemistry Ovarian Follicle Rats Rats, Sprague-Dawley Receptor, TIE-2 Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 |
| description |
This study investigated the protein expression and cellular localization of ANGPT1, ANGPT2, and their receptor TEK, as well as vascular endothelial growth factor A (VEGFA) and its receptor KDR (VEGFR2) during folliculogenesis. To obtain follicles at different stages for immunochemistry and western analyses, we used prepubertal untreated, diethylstilbestrol- and equine chorionic gonadotropin-treated rats. To confirm that these hormonal treatments reflect physiological change, we used non-treated adult rats. No expression of ANGPT1 was observed in granulosa cells (Gc) from immature hormone-treated and non-treated rats at any follicular stage. By contrast, ANGPT1 expression in theca cells (Tc) increased with follicular maturation. ANGPT2 protein was either absent or weakly expressed in Gc at all follicular stages. In Tc, minimal expression of ANGPT2 protein was detected in the preantral follicle (PF), whereas it was stronger in the early antral follicle (EAF) and preovulatory follicle (POF). TEK staining was absent in Gc but was intense in Tc at every follicular stage. Staining for VEGFA was either absent or weakly present in Gc and Tc in PF and EAF, although in POF it was stronger in Gc and Tc. Staining for KDR was absent in Gc and very low in Tc from PF. Gc and Tc of EAF showed positive staining for KDR and in POF the staining was stronger. These results were confirmed by western immunoblot. A similar pattern of expression of these proteins was observed in cycling rats. In conclusion, we observed that the protein expression of ANGPT1, ANGPT2, VEGFA and their receptors increased during follicular development in rats. © 2009 Society for Reproduction and Fertility. |
| format |
JOUR |
| author |
Abramovich, D. Celin, A.R. Hernandez, F. Tesone, M. Parborell, F. |
| author_facet |
Abramovich, D. Celin, A.R. Hernandez, F. Tesone, M. Parborell, F. |
| author_sort |
Abramovich, D. |
| title |
Spatiotemporal analysis of the protein expression of angiogenic factors and their related receptors during folliculogenesis in rats with and without hormonal treatment |
| title_short |
Spatiotemporal analysis of the protein expression of angiogenic factors and their related receptors during folliculogenesis in rats with and without hormonal treatment |
| title_full |
Spatiotemporal analysis of the protein expression of angiogenic factors and their related receptors during folliculogenesis in rats with and without hormonal treatment |
| title_fullStr |
Spatiotemporal analysis of the protein expression of angiogenic factors and their related receptors during folliculogenesis in rats with and without hormonal treatment |
| title_full_unstemmed |
Spatiotemporal analysis of the protein expression of angiogenic factors and their related receptors during folliculogenesis in rats with and without hormonal treatment |
| title_sort |
spatiotemporal analysis of the protein expression of angiogenic factors and their related receptors during folliculogenesis in rats with and without hormonal treatment |
| url |
http://hdl.handle.net/20.500.12110/paper_14701626_v137_n2_p309_Abramovich |
| work_keys_str_mv |
AT abramovichd spatiotemporalanalysisoftheproteinexpressionofangiogenicfactorsandtheirrelatedreceptorsduringfolliculogenesisinratswithandwithouthormonaltreatment AT celinar spatiotemporalanalysisoftheproteinexpressionofangiogenicfactorsandtheirrelatedreceptorsduringfolliculogenesisinratswithandwithouthormonaltreatment AT hernandezf spatiotemporalanalysisoftheproteinexpressionofangiogenicfactorsandtheirrelatedreceptorsduringfolliculogenesisinratswithandwithouthormonaltreatment AT tesonem spatiotemporalanalysisoftheproteinexpressionofangiogenicfactorsandtheirrelatedreceptorsduringfolliculogenesisinratswithandwithouthormonaltreatment AT parborellf spatiotemporalanalysisoftheproteinexpressionofangiogenicfactorsandtheirrelatedreceptorsduringfolliculogenesisinratswithandwithouthormonaltreatment |
| _version_ |
1807314910467588096 |