Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis

We analyzed the influence of the redox global regulator Anr on the accumulation of poly(3-hydroxybutyrate) (PHB) in Pseudomonas extremaustralis. Anr regulates a set of genes in the aerobic-anaerobic transition including genes involved in nitrate reduction and arginine fermentation. An anr mutant was...

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Autores principales:	Tribelli, P.M., Méndez, B.S., López, N.I.
Formato:	JOUR
Materias:	Anr Microaerobiosis Poly(3-hydroxybutyrate) Pseudomonas nitrate poly(3 hydroxybutyric acid) aerobic bacterium aerobic metabolism anaerobic bacterium Anr gene article bacterial gene bacterial genetics bacterial growth bacterial strain bacterium culture binding site bioreactor controlled study electron gene control gene expression gene inactivation gene location genetic association mutant nonhuman nucleotide sequence oxygen sensitive global regulator phaC gene phaR gene phenotype polymerase chain reaction Pseudomonas extremaustralis real time polymerase chain reaction wild type Bacterial Proteins Base Sequence Bioreactors DNA-Binding Proteins Gene Expression Regulation Hydroxybutyrates Molecular Sequence Data Mutation Oxygen Polyesters Promoter Regions, Genetic Sequence Alignment
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_14641801_v19_n4_p180_Tribelli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_14641801_v19_n4_p180_Tribelli
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spelling	todo:paper_14641801_v19_n4_p180_Tribelli2023-10-03T16:17:06Z Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis Tribelli, P.M. Méndez, B.S. López, N.I. Anr Microaerobiosis Poly(3-hydroxybutyrate) Pseudomonas nitrate poly(3 hydroxybutyric acid) aerobic bacterium aerobic metabolism anaerobic bacterium Anr gene article bacterial gene bacterial genetics bacterial growth bacterial strain bacterium culture binding site bioreactor controlled study electron gene control gene expression gene inactivation gene location genetic association mutant nonhuman nucleotide sequence oxygen sensitive global regulator phaC gene phaR gene phenotype polymerase chain reaction Pseudomonas Pseudomonas extremaustralis real time polymerase chain reaction wild type Bacterial Proteins Base Sequence Bioreactors DNA-Binding Proteins Gene Expression Regulation Hydroxybutyrates Molecular Sequence Data Mutation Oxygen Polyesters Promoter Regions, Genetic Pseudomonas Sequence Alignment Pseudomonas We analyzed the influence of the redox global regulator Anr on the accumulation of poly(3-hydroxybutyrate) (PHB) in Pseudomonas extremaustralis. Anr regulates a set of genes in the aerobic-anaerobic transition including genes involved in nitrate reduction and arginine fermentation. An anr mutant was constructed using PCR-based strategies. The wild-type strain was able to grow in both microaerobic and anaerobic conditions using nitrate as the terminal electron acceptor while the mutant strain was unable to grow under anaerobic conditions. In bioreactor cultures, PHB content in the wild-type strain was higher in microaerobic and anaerobic cultures compared with highly aerated cultures. The mutant strain showed decreased PHB levels in both aerobic and microaerobic conditions compared with the wild-type strain. Inactivation of anr led to decreased expression of phaC and phaR genes as demonstrated in real-time RT-PCR experiments. Associated with the PHB gene region, two putative binding sites for Anr were found that, in line with the phenotype observed in bioreactor cultures, suggest a role of this regulator in PHB biosynthesis. copyright © 2010 S. Karger AG, Basel. Fil:Tribelli, P.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14641801_v19_n4_p180_Tribelli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Anr Microaerobiosis Poly(3-hydroxybutyrate) Pseudomonas nitrate poly(3 hydroxybutyric acid) aerobic bacterium aerobic metabolism anaerobic bacterium Anr gene article bacterial gene bacterial genetics bacterial growth bacterial strain bacterium culture binding site bioreactor controlled study electron gene control gene expression gene inactivation gene location genetic association mutant nonhuman nucleotide sequence oxygen sensitive global regulator phaC gene phaR gene phenotype polymerase chain reaction Pseudomonas Pseudomonas extremaustralis real time polymerase chain reaction wild type Bacterial Proteins Base Sequence Bioreactors DNA-Binding Proteins Gene Expression Regulation Hydroxybutyrates Molecular Sequence Data Mutation Oxygen Polyesters Promoter Regions, Genetic Pseudomonas Sequence Alignment Pseudomonas
spellingShingle	Anr Microaerobiosis Poly(3-hydroxybutyrate) Pseudomonas nitrate poly(3 hydroxybutyric acid) aerobic bacterium aerobic metabolism anaerobic bacterium Anr gene article bacterial gene bacterial genetics bacterial growth bacterial strain bacterium culture binding site bioreactor controlled study electron gene control gene expression gene inactivation gene location genetic association mutant nonhuman nucleotide sequence oxygen sensitive global regulator phaC gene phaR gene phenotype polymerase chain reaction Pseudomonas Pseudomonas extremaustralis real time polymerase chain reaction wild type Bacterial Proteins Base Sequence Bioreactors DNA-Binding Proteins Gene Expression Regulation Hydroxybutyrates Molecular Sequence Data Mutation Oxygen Polyesters Promoter Regions, Genetic Pseudomonas Sequence Alignment Pseudomonas Tribelli, P.M. Méndez, B.S. López, N.I. Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis
topic_facet	Anr Microaerobiosis Poly(3-hydroxybutyrate) Pseudomonas nitrate poly(3 hydroxybutyric acid) aerobic bacterium aerobic metabolism anaerobic bacterium Anr gene article bacterial gene bacterial genetics bacterial growth bacterial strain bacterium culture binding site bioreactor controlled study electron gene control gene expression gene inactivation gene location genetic association mutant nonhuman nucleotide sequence oxygen sensitive global regulator phaC gene phaR gene phenotype polymerase chain reaction Pseudomonas Pseudomonas extremaustralis real time polymerase chain reaction wild type Bacterial Proteins Base Sequence Bioreactors DNA-Binding Proteins Gene Expression Regulation Hydroxybutyrates Molecular Sequence Data Mutation Oxygen Polyesters Promoter Regions, Genetic Pseudomonas Sequence Alignment Pseudomonas
description	We analyzed the influence of the redox global regulator Anr on the accumulation of poly(3-hydroxybutyrate) (PHB) in Pseudomonas extremaustralis. Anr regulates a set of genes in the aerobic-anaerobic transition including genes involved in nitrate reduction and arginine fermentation. An anr mutant was constructed using PCR-based strategies. The wild-type strain was able to grow in both microaerobic and anaerobic conditions using nitrate as the terminal electron acceptor while the mutant strain was unable to grow under anaerobic conditions. In bioreactor cultures, PHB content in the wild-type strain was higher in microaerobic and anaerobic cultures compared with highly aerated cultures. The mutant strain showed decreased PHB levels in both aerobic and microaerobic conditions compared with the wild-type strain. Inactivation of anr led to decreased expression of phaC and phaR genes as demonstrated in real-time RT-PCR experiments. Associated with the PHB gene region, two putative binding sites for Anr were found that, in line with the phenotype observed in bioreactor cultures, suggest a role of this regulator in PHB biosynthesis. copyright © 2010 S. Karger AG, Basel.
format	JOUR
author	Tribelli, P.M. Méndez, B.S. López, N.I.
author_facet	Tribelli, P.M. Méndez, B.S. López, N.I.
author_sort	Tribelli, P.M.
title	Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis
title_short	Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis
title_full	Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis
title_fullStr	Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis
title_full_unstemmed	Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis
title_sort	oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-hydroxybutyrate) in pseudomonas extremaustralis
url	http://hdl.handle.net/20.500.12110/paper_14641801_v19_n4_p180_Tribelli
work_keys_str_mv	AT tribellipm oxygensensitiveglobalregulatoranrisinvolvedinthebiosynthesisofpoly3hydroxybutyrateinpseudomonasextremaustralis AT mendezbs oxygensensitiveglobalregulatoranrisinvolvedinthebiosynthesisofpoly3hydroxybutyrateinpseudomonasextremaustralis AT lopezni oxygensensitiveglobalregulatoranrisinvolvedinthebiosynthesisofpoly3hydroxybutyrateinpseudomonasextremaustralis
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Oxygen-sensitive global regulator, anr, is involved in the biosynthesis of poly(3-Hydroxybutyrate) in pseudomonas extremaustralis

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