Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves
Temperate grasses, such as wheat, become compact plants with small thick leaves after exposure to low temperature. These responses are associated with cold hardiness, but their underlying mechanisms remain largely unknown. Here we analyse the effects of low temperature on leaf morpho-anatomical stru...
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todo:paper_14358603_v21_n_p84_Lorenzo2023-10-03T16:15:52Z Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves Lorenzo, M. Pinedo, M.L. Equiza, M.A. Fernández, P.V. Ciancia, M. Ganem, D.G. Tognetti, J.A. Cell elongation cell wall cold hardiness hemicellulose peroxidase wheat anatomy cell component cellulose cold tolerance cultivar enzyme activity grass leaf morphology low temperature wheat Poaceae Triticum aestivum hemicellulose peroxidase plant protein polysaccharide anatomy and histology cell wall cold metabolism physiology plant leaf season wheat Cell Wall Cold Temperature Peroxidase Plant Leaves Plant Proteins Polysaccharides Seasons Triticum Temperate grasses, such as wheat, become compact plants with small thick leaves after exposure to low temperature. These responses are associated with cold hardiness, but their underlying mechanisms remain largely unknown. Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability. A combined microscopy and biochemical approach was applied to study actively growing leaves of winter (ProINTA-Pincén) and spring (Buck-Patacón) wheat developed under constant warm (25 °C) or cool (5 °C) temperature. Cold-grown plants had shorter leaves but longer inter-stomatal epidermal cells than warm-grown plants. They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose. These effects were more pronounced in the winter cultivar (Pincén). Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf. This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C. The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening. New insights are presented on the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses. © 2018 German Society for Plant Sciences and The Royal Botanical Society of the Netherlands JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14358603_v21_n_p84_Lorenzo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cell elongation cell wall cold hardiness hemicellulose peroxidase wheat anatomy cell component cellulose cold tolerance cultivar enzyme activity grass leaf morphology low temperature wheat Poaceae Triticum aestivum hemicellulose peroxidase plant protein polysaccharide anatomy and histology cell wall cold metabolism physiology plant leaf season wheat Cell Wall Cold Temperature Peroxidase Plant Leaves Plant Proteins Polysaccharides Seasons Triticum |
| spellingShingle |
Cell elongation cell wall cold hardiness hemicellulose peroxidase wheat anatomy cell component cellulose cold tolerance cultivar enzyme activity grass leaf morphology low temperature wheat Poaceae Triticum aestivum hemicellulose peroxidase plant protein polysaccharide anatomy and histology cell wall cold metabolism physiology plant leaf season wheat Cell Wall Cold Temperature Peroxidase Plant Leaves Plant Proteins Polysaccharides Seasons Triticum Lorenzo, M. Pinedo, M.L. Equiza, M.A. Fernández, P.V. Ciancia, M. Ganem, D.G. Tognetti, J.A. Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves |
| topic_facet |
Cell elongation cell wall cold hardiness hemicellulose peroxidase wheat anatomy cell component cellulose cold tolerance cultivar enzyme activity grass leaf morphology low temperature wheat Poaceae Triticum aestivum hemicellulose peroxidase plant protein polysaccharide anatomy and histology cell wall cold metabolism physiology plant leaf season wheat Cell Wall Cold Temperature Peroxidase Plant Leaves Plant Proteins Polysaccharides Seasons Triticum |
| description |
Temperate grasses, such as wheat, become compact plants with small thick leaves after exposure to low temperature. These responses are associated with cold hardiness, but their underlying mechanisms remain largely unknown. Here we analyse the effects of low temperature on leaf morpho-anatomical structure, cell wall composition and activity of extracellular peroxidases, which play key roles in cell elongation and cell wall thickening, in two wheat cultivars with contrasting cold-hardening ability. A combined microscopy and biochemical approach was applied to study actively growing leaves of winter (ProINTA-Pincén) and spring (Buck-Patacón) wheat developed under constant warm (25 °C) or cool (5 °C) temperature. Cold-grown plants had shorter leaves but longer inter-stomatal epidermal cells than warm-grown plants. They had thicker walls in metaxylem vessels and mestome sheath cells, paralleled with accumulation of wall components, predominantly hemicellulose. These effects were more pronounced in the winter cultivar (Pincén). Cold also induced a sharp decrease in apoplastic peroxidase activity within the leaf elongating zone of Pincén, and a three-fold increase in the distal mature zone of the leaf. This was consistent with the enhanced cell length and thicker cell walls in this cultivar at 5 °C. The different response to low temperature of apoplastic peroxidase activity and hemicellulose between leaf zones and cultivar types suggests they might play a central role in the development of cold-induced compact morphology and cold hardening. New insights are presented on the potential temperature-driven role of peroxidases and hemicellulose in cell wall dynamics of grasses. © 2018 German Society for Plant Sciences and The Royal Botanical Society of the Netherlands |
| format |
JOUR |
| author |
Lorenzo, M. Pinedo, M.L. Equiza, M.A. Fernández, P.V. Ciancia, M. Ganem, D.G. Tognetti, J.A. |
| author_facet |
Lorenzo, M. Pinedo, M.L. Equiza, M.A. Fernández, P.V. Ciancia, M. Ganem, D.G. Tognetti, J.A. |
| author_sort |
Lorenzo, M. |
| title |
Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves |
| title_short |
Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves |
| title_full |
Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves |
| title_fullStr |
Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves |
| title_full_unstemmed |
Changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves |
| title_sort |
changes in apoplastic peroxidase activity and cell wall composition are associated with cold-induced morpho-anatomical plasticity of wheat leaves |
| url |
http://hdl.handle.net/20.500.12110/paper_14358603_v21_n_p84_Lorenzo |
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