Utility of Genetic Markers and Morphology for Species Discrimination within the Order Tintinnida (Ciliophora, Spirotrichea)
We evaluated the small- and large-subunit rDNA (SSU and LSU, respectively) for their ability to discriminate morphospecies of tintinnid ciliates. Multiple individuals from 29 morphospecies were identified according to microscopically-observed characteristics of the lorica, and then sequenced for bot...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_14344610_v164_n1_p24_Santoferrara |
| Aporte de: |
| id |
todo:paper_14344610_v164_n1_p24_Santoferrara |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_14344610_v164_n1_p24_Santoferrara2023-10-03T16:14:25Z Utility of Genetic Markers and Morphology for Species Discrimination within the Order Tintinnida (Ciliophora, Spirotrichea) Santoferrara, L.F. McManus, G.B. Alder, V.A. Barcoding Ciliophora LSU rDNA SSU rDNA V4 V9 protozoal DNA protozoal RNA ribosome DNA ribosome RNA RNA 18S RNA, ribosomal, 26S article chemistry ciliate classification cluster analysis cytology DNA sequence genetics microscopy molecular genetics nucleotide sequence phylogeny RNA gene Ciliophora Cluster Analysis DNA, Protozoan DNA, Ribosomal Genes, rRNA Microscopy Molecular Sequence Data Phylogeny RNA, Protozoan RNA, Ribosomal RNA, Ribosomal, 18S Sequence Analysis, DNA Ciliophora Lorica Spirotrichea Tintinnida We evaluated the small- and large-subunit rDNA (SSU and LSU, respectively) for their ability to discriminate morphospecies of tintinnid ciliates. Multiple individuals from 29 morphospecies were identified according to microscopically-observed characteristics of the lorica, and then sequenced for both loci (21 new species for SSU and all of them new for LSU). Sequences from public databases were included in our analyses, and two hypervariable SSU regions (V4 and V9) were separately examined. Of the four regions, LSU is the most useful as a potential barcoding tool. It showed a gap in distances within and between species, and discriminated the maximum number of phylotypes (86% at 1% cut-off). SSU and V4 were less consistent, sometimes lumping together very distinctive morphospecies, even at the 1% level of sequence divergence. V9 was the least reliable marker in delimitating morphospecies. The agreement in sequences and morphology suggests that the lorica is useful for species discrimination, even in agglomerated forms. However, the observation of both genetically constant yet polymorphic groups of species, as well as similar morphospecies with divergent sequences, indicates that previous taxonomic schemes are complementary to the emerging molecular database. © 2011 Elsevier GmbH. Fil:Santoferrara, L.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alder, V.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14344610_v164_n1_p24_Santoferrara |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Barcoding Ciliophora LSU rDNA SSU rDNA V4 V9 protozoal DNA protozoal RNA ribosome DNA ribosome RNA RNA 18S RNA, ribosomal, 26S article chemistry ciliate classification cluster analysis cytology DNA sequence genetics microscopy molecular genetics nucleotide sequence phylogeny RNA gene Ciliophora Cluster Analysis DNA, Protozoan DNA, Ribosomal Genes, rRNA Microscopy Molecular Sequence Data Phylogeny RNA, Protozoan RNA, Ribosomal RNA, Ribosomal, 18S Sequence Analysis, DNA Ciliophora Lorica Spirotrichea Tintinnida |
| spellingShingle |
Barcoding Ciliophora LSU rDNA SSU rDNA V4 V9 protozoal DNA protozoal RNA ribosome DNA ribosome RNA RNA 18S RNA, ribosomal, 26S article chemistry ciliate classification cluster analysis cytology DNA sequence genetics microscopy molecular genetics nucleotide sequence phylogeny RNA gene Ciliophora Cluster Analysis DNA, Protozoan DNA, Ribosomal Genes, rRNA Microscopy Molecular Sequence Data Phylogeny RNA, Protozoan RNA, Ribosomal RNA, Ribosomal, 18S Sequence Analysis, DNA Ciliophora Lorica Spirotrichea Tintinnida Santoferrara, L.F. McManus, G.B. Alder, V.A. Utility of Genetic Markers and Morphology for Species Discrimination within the Order Tintinnida (Ciliophora, Spirotrichea) |
| topic_facet |
Barcoding Ciliophora LSU rDNA SSU rDNA V4 V9 protozoal DNA protozoal RNA ribosome DNA ribosome RNA RNA 18S RNA, ribosomal, 26S article chemistry ciliate classification cluster analysis cytology DNA sequence genetics microscopy molecular genetics nucleotide sequence phylogeny RNA gene Ciliophora Cluster Analysis DNA, Protozoan DNA, Ribosomal Genes, rRNA Microscopy Molecular Sequence Data Phylogeny RNA, Protozoan RNA, Ribosomal RNA, Ribosomal, 18S Sequence Analysis, DNA Ciliophora Lorica Spirotrichea Tintinnida |
| description |
We evaluated the small- and large-subunit rDNA (SSU and LSU, respectively) for their ability to discriminate morphospecies of tintinnid ciliates. Multiple individuals from 29 morphospecies were identified according to microscopically-observed characteristics of the lorica, and then sequenced for both loci (21 new species for SSU and all of them new for LSU). Sequences from public databases were included in our analyses, and two hypervariable SSU regions (V4 and V9) were separately examined. Of the four regions, LSU is the most useful as a potential barcoding tool. It showed a gap in distances within and between species, and discriminated the maximum number of phylotypes (86% at 1% cut-off). SSU and V4 were less consistent, sometimes lumping together very distinctive morphospecies, even at the 1% level of sequence divergence. V9 was the least reliable marker in delimitating morphospecies. The agreement in sequences and morphology suggests that the lorica is useful for species discrimination, even in agglomerated forms. However, the observation of both genetically constant yet polymorphic groups of species, as well as similar morphospecies with divergent sequences, indicates that previous taxonomic schemes are complementary to the emerging molecular database. © 2011 Elsevier GmbH. |
| format |
JOUR |
| author |
Santoferrara, L.F. McManus, G.B. Alder, V.A. |
| author_facet |
Santoferrara, L.F. McManus, G.B. Alder, V.A. |
| author_sort |
Santoferrara, L.F. |
| title |
Utility of Genetic Markers and Morphology for Species Discrimination within the Order Tintinnida (Ciliophora, Spirotrichea) |
| title_short |
Utility of Genetic Markers and Morphology for Species Discrimination within the Order Tintinnida (Ciliophora, Spirotrichea) |
| title_full |
Utility of Genetic Markers and Morphology for Species Discrimination within the Order Tintinnida (Ciliophora, Spirotrichea) |
| title_fullStr |
Utility of Genetic Markers and Morphology for Species Discrimination within the Order Tintinnida (Ciliophora, Spirotrichea) |
| title_full_unstemmed |
Utility of Genetic Markers and Morphology for Species Discrimination within the Order Tintinnida (Ciliophora, Spirotrichea) |
| title_sort |
utility of genetic markers and morphology for species discrimination within the order tintinnida (ciliophora, spirotrichea) |
| url |
http://hdl.handle.net/20.500.12110/paper_14344610_v164_n1_p24_Santoferrara |
| work_keys_str_mv |
AT santoferraralf utilityofgeneticmarkersandmorphologyforspeciesdiscriminationwithintheordertintinnidaciliophoraspirotrichea AT mcmanusgb utilityofgeneticmarkersandmorphologyforspeciesdiscriminationwithintheordertintinnidaciliophoraspirotrichea AT alderva utilityofgeneticmarkersandmorphologyforspeciesdiscriminationwithintheordertintinnidaciliophoraspirotrichea |
| _version_ |
1807317928573403136 |