Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae)

The neo-X and neo-Y sex chromosomes of Dysdercus albofasciatus represent a unique model for the study of early stages of sex chromosome evolution since they retained the ability to pair and recombine, in contrast to sex chromosomes in most Heteroptera. Here we examined structure, molecular different...

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Autores principales:	Bressa, M.J., Papeschi, A.G., Vítková, M., Kubíková, S., Fuková, I., Pigozzi, M.I., Marec, F.
Formato:	JOUR
Materias:	Chromosome painting Evolution GISH NOR Ribosomal DNA Sex chromosomes Synaptonemal complex Zoo-FISH cohesin ribosome DNA 4',6 diamidino 2 phenylindole fluorescent dye indole derivative animal experiment animal tissue article autosome chromosome band chromosome C band chromosome NOR chromosome painting chromosome pairing controlled study Dysdercus albofasciatus Dysdercus chaquensis Dysdercus ruficollis fluorescence in situ hybridization genomic in situ hybridization Heteroptera immunohistochemistry karyotype karyotype evolution male nonhuman priority journal sex chromosome species difference synaptonemal complex X chromosome Y chromosome animal biotinylation chemistry chromosome chromosome banding pattern DNA probe female genetics karyotyping meiosis metabolism metaphase molecular evolution Dysdercus Pyrrhocoridae Wupatkius semo Animals Biotinylation Chromosome Banding Chromosome Painting Chromosomes DNA Probes DNA, Ribosomal Evolution, Molecular Female Fluorescent Dyes Immunohistochemistry In Situ Hybridization, Fluorescence Indoles Karyotyping Male Meiosis Metaphase Nucleolus Organizer Region Species Specificity Synaptonemal Complex X Chromosome Y Chromosome
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_14248581_v125_n4_p292_Bressa
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_14248581_v125_n4_p292_Bressa2023-10-03T16:13:47Z Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae) Bressa, M.J. Papeschi, A.G. Vítková, M. Kubíková, S. Fuková, I. Pigozzi, M.I. Marec, F. Chromosome painting Evolution GISH NOR Ribosomal DNA Sex chromosomes Synaptonemal complex Zoo-FISH cohesin ribosome DNA 4',6 diamidino 2 phenylindole fluorescent dye indole derivative animal experiment animal tissue article autosome chromosome band chromosome C band chromosome NOR chromosome painting chromosome pairing controlled study Dysdercus albofasciatus Dysdercus chaquensis Dysdercus ruficollis fluorescence in situ hybridization genomic in situ hybridization Heteroptera immunohistochemistry karyotype karyotype evolution male nonhuman priority journal sex chromosome species difference synaptonemal complex X chromosome Y chromosome animal biotinylation chemistry chromosome chromosome banding pattern DNA probe female genetics karyotyping meiosis metabolism metaphase molecular evolution X chromosome Y chromosome Dysdercus Dysdercus ruficollis Heteroptera Pyrrhocoridae Wupatkius semo Animals Biotinylation Chromosome Banding Chromosome Painting Chromosomes DNA Probes DNA, Ribosomal Evolution, Molecular Female Fluorescent Dyes Heteroptera Immunohistochemistry In Situ Hybridization, Fluorescence Indoles Karyotyping Male Meiosis Metaphase Nucleolus Organizer Region Species Specificity Synaptonemal Complex X Chromosome Y Chromosome The neo-X and neo-Y sex chromosomes of Dysdercus albofasciatus represent a unique model for the study of early stages of sex chromosome evolution since they retained the ability to pair and recombine, in contrast to sex chromosomes in most Heteroptera. Here we examined structure, molecular differentiation, and meiotic behaviour of the D. albofasciatus neo-sex chromosomes. Two related species with the ancestral X0 system, D. chaquensis and D. ruficollis, were used for a comparison. In D. albofasciatus, 2 nucleolar organizer regions (NORs) were identified on the neo-X chromosome using fluorescence in situ hybridization (FISH) with an rDNA probe, whereas a single NOR was found on an autosomal pair in the other 2 species. Genomic in situ hybridization (GISH) differentiated a part of the original X in the neo-X chromosome but not the neo-Y chromosome. The same segment of the neo-X chromosome was identified by Zoo-FISH with a chromosome painting probe derived from the X chromosome of D. ruficollis, indicating that this part is conserved between the species. Immunostaining against the cohesin subunit SMC3 revealed that only terminal regions of the D. albofasciatus neo-Xneo-Y bivalent pair and form a synaptonemal complex, which is in keeping with the occurrence of terminal chiasmata, whereas the interstitial region forms a large loop indicating the absence of homology. These results support the hypothesis that the neo-X chromosome evolved by insertion of the original X chromosome into 1 NOR-bearing autosome in an ancestor carrying the X0 system. As a consequence, the homologue of this NOR-autosome became the neo-Y chromosome. A subsequent inversion followed by transposition of the NOR located on the neo-Y onto the neo-X chromosome resulted in the present neo-sex chromosome system in D. albofasciatus. Copyright © 2009 S. Karger AG, Basel. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14248581_v125_n4_p292_Bressa
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Chromosome painting Evolution GISH NOR Ribosomal DNA Sex chromosomes Synaptonemal complex Zoo-FISH cohesin ribosome DNA 4',6 diamidino 2 phenylindole fluorescent dye indole derivative animal experiment animal tissue article autosome chromosome band chromosome C band chromosome NOR chromosome painting chromosome pairing controlled study Dysdercus albofasciatus Dysdercus chaquensis Dysdercus ruficollis fluorescence in situ hybridization genomic in situ hybridization Heteroptera immunohistochemistry karyotype karyotype evolution male nonhuman priority journal sex chromosome species difference synaptonemal complex X chromosome Y chromosome animal biotinylation chemistry chromosome chromosome banding pattern DNA probe female genetics karyotyping meiosis metabolism metaphase molecular evolution X chromosome Y chromosome Dysdercus Dysdercus ruficollis Heteroptera Pyrrhocoridae Wupatkius semo Animals Biotinylation Chromosome Banding Chromosome Painting Chromosomes DNA Probes DNA, Ribosomal Evolution, Molecular Female Fluorescent Dyes Heteroptera Immunohistochemistry In Situ Hybridization, Fluorescence Indoles Karyotyping Male Meiosis Metaphase Nucleolus Organizer Region Species Specificity Synaptonemal Complex X Chromosome Y Chromosome
spellingShingle	Chromosome painting Evolution GISH NOR Ribosomal DNA Sex chromosomes Synaptonemal complex Zoo-FISH cohesin ribosome DNA 4',6 diamidino 2 phenylindole fluorescent dye indole derivative animal experiment animal tissue article autosome chromosome band chromosome C band chromosome NOR chromosome painting chromosome pairing controlled study Dysdercus albofasciatus Dysdercus chaquensis Dysdercus ruficollis fluorescence in situ hybridization genomic in situ hybridization Heteroptera immunohistochemistry karyotype karyotype evolution male nonhuman priority journal sex chromosome species difference synaptonemal complex X chromosome Y chromosome animal biotinylation chemistry chromosome chromosome banding pattern DNA probe female genetics karyotyping meiosis metabolism metaphase molecular evolution X chromosome Y chromosome Dysdercus Dysdercus ruficollis Heteroptera Pyrrhocoridae Wupatkius semo Animals Biotinylation Chromosome Banding Chromosome Painting Chromosomes DNA Probes DNA, Ribosomal Evolution, Molecular Female Fluorescent Dyes Heteroptera Immunohistochemistry In Situ Hybridization, Fluorescence Indoles Karyotyping Male Meiosis Metaphase Nucleolus Organizer Region Species Specificity Synaptonemal Complex X Chromosome Y Chromosome Bressa, M.J. Papeschi, A.G. Vítková, M. Kubíková, S. Fuková, I. Pigozzi, M.I. Marec, F. Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae)
topic_facet	Chromosome painting Evolution GISH NOR Ribosomal DNA Sex chromosomes Synaptonemal complex Zoo-FISH cohesin ribosome DNA 4',6 diamidino 2 phenylindole fluorescent dye indole derivative animal experiment animal tissue article autosome chromosome band chromosome C band chromosome NOR chromosome painting chromosome pairing controlled study Dysdercus albofasciatus Dysdercus chaquensis Dysdercus ruficollis fluorescence in situ hybridization genomic in situ hybridization Heteroptera immunohistochemistry karyotype karyotype evolution male nonhuman priority journal sex chromosome species difference synaptonemal complex X chromosome Y chromosome animal biotinylation chemistry chromosome chromosome banding pattern DNA probe female genetics karyotyping meiosis metabolism metaphase molecular evolution X chromosome Y chromosome Dysdercus Dysdercus ruficollis Heteroptera Pyrrhocoridae Wupatkius semo Animals Biotinylation Chromosome Banding Chromosome Painting Chromosomes DNA Probes DNA, Ribosomal Evolution, Molecular Female Fluorescent Dyes Heteroptera Immunohistochemistry In Situ Hybridization, Fluorescence Indoles Karyotyping Male Meiosis Metaphase Nucleolus Organizer Region Species Specificity Synaptonemal Complex X Chromosome Y Chromosome
description	The neo-X and neo-Y sex chromosomes of Dysdercus albofasciatus represent a unique model for the study of early stages of sex chromosome evolution since they retained the ability to pair and recombine, in contrast to sex chromosomes in most Heteroptera. Here we examined structure, molecular differentiation, and meiotic behaviour of the D. albofasciatus neo-sex chromosomes. Two related species with the ancestral X0 system, D. chaquensis and D. ruficollis, were used for a comparison. In D. albofasciatus, 2 nucleolar organizer regions (NORs) were identified on the neo-X chromosome using fluorescence in situ hybridization (FISH) with an rDNA probe, whereas a single NOR was found on an autosomal pair in the other 2 species. Genomic in situ hybridization (GISH) differentiated a part of the original X in the neo-X chromosome but not the neo-Y chromosome. The same segment of the neo-X chromosome was identified by Zoo-FISH with a chromosome painting probe derived from the X chromosome of D. ruficollis, indicating that this part is conserved between the species. Immunostaining against the cohesin subunit SMC3 revealed that only terminal regions of the D. albofasciatus neo-Xneo-Y bivalent pair and form a synaptonemal complex, which is in keeping with the occurrence of terminal chiasmata, whereas the interstitial region forms a large loop indicating the absence of homology. These results support the hypothesis that the neo-X chromosome evolved by insertion of the original X chromosome into 1 NOR-bearing autosome in an ancestor carrying the X0 system. As a consequence, the homologue of this NOR-autosome became the neo-Y chromosome. A subsequent inversion followed by transposition of the NOR located on the neo-Y onto the neo-X chromosome resulted in the present neo-sex chromosome system in D. albofasciatus. Copyright © 2009 S. Karger AG, Basel.
format	JOUR
author	Bressa, M.J. Papeschi, A.G. Vítková, M. Kubíková, S. Fuková, I. Pigozzi, M.I. Marec, F.
author_facet	Bressa, M.J. Papeschi, A.G. Vítková, M. Kubíková, S. Fuková, I. Pigozzi, M.I. Marec, F.
author_sort	Bressa, M.J.
title	Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae)
title_short	Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae)
title_full	Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae)
title_fullStr	Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae)
title_full_unstemmed	Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae)
title_sort	sex chromosome evolution in cotton stainers of the genus dysdercus (heteroptera: pyrrhocoridae)
url	http://hdl.handle.net/20.500.12110/paper_14248581_v125_n4_p292_Bressa
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Sex chromosome evolution in cotton stainers of the genus dysdercus (Heteroptera: Pyrrhocoridae)

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