The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses
We describe for the first time the karyotype of the black howler monkey, Alouatta pigra. Conventional staining, G- and C-banding, and fluorescence in situ hybridization (FISH) with a peptide nucleic acid (PNA) pantelomeric probe were performed. Eight free ranging adult individuals, four males and fo...
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todo:paper_14248581_v122_n2_p103_Steinberg2023-10-03T16:13:47Z The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses Steinberg, E.R. Cortés-Ortiz, L. Nieves, M. Bolzán, A.D. García-Orduña, F. Hermida-Lagunes, J. Canales-Espinosa, D. Mudry, M.D. peptide nucleic acid acrocentric chromosome Alouatta animal cell article autosome centromere chromosome bivalent chromosome C band chromosome G band chromosome identification chromosome painting chromosome pairing chromosome structure diploidy DNA sequence female fluorescence in situ hybridization karyotype male meiosis metaphase mitosis morphology nonhuman nucleic acid probe priority journal telomere Animals Central America Female Karyotyping Male Meiosis Mitosis Platyrrhini Alouatta caraya Alouatta pigra Platyrrhini Primates We describe for the first time the karyotype of the black howler monkey, Alouatta pigra. Conventional staining, G- and C-banding, and fluorescence in situ hybridization (FISH) with a peptide nucleic acid (PNA) pantelomeric probe were performed. Eight free ranging adult individuals, four males and four females, within the natural distribution of the species presented a diploid karyotype with 2n = 58. Mitotic analyses showed an autosomal complement composed of 6 submetacentric, 3 metacentric, and 19 acrocentric chromosome pairs for females, and 6 submetacentric, 3 metacentric, and 18 acrocentric pairs for males. Meiotic analyses in males revealed 27 autosomal bivalents and a quadrivalent composed of a submetacentric X1 and acrocentric X 2, Y1, and Y2. The G-banded karyotype allowed us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the quadrivalent components. C-banding technique in metaphase I corroborated the structure of the quadrivalent showing four C+ centromeres. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. Copyright © 2008 S. Karger AG. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14248581_v122_n2_p103_Steinberg |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
peptide nucleic acid acrocentric chromosome Alouatta animal cell article autosome centromere chromosome bivalent chromosome C band chromosome G band chromosome identification chromosome painting chromosome pairing chromosome structure diploidy DNA sequence female fluorescence in situ hybridization karyotype male meiosis metaphase mitosis morphology nonhuman nucleic acid probe priority journal telomere Animals Central America Female Karyotyping Male Meiosis Mitosis Platyrrhini Alouatta caraya Alouatta pigra Platyrrhini Primates |
| spellingShingle |
peptide nucleic acid acrocentric chromosome Alouatta animal cell article autosome centromere chromosome bivalent chromosome C band chromosome G band chromosome identification chromosome painting chromosome pairing chromosome structure diploidy DNA sequence female fluorescence in situ hybridization karyotype male meiosis metaphase mitosis morphology nonhuman nucleic acid probe priority journal telomere Animals Central America Female Karyotyping Male Meiosis Mitosis Platyrrhini Alouatta caraya Alouatta pigra Platyrrhini Primates Steinberg, E.R. Cortés-Ortiz, L. Nieves, M. Bolzán, A.D. García-Orduña, F. Hermida-Lagunes, J. Canales-Espinosa, D. Mudry, M.D. The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses |
| topic_facet |
peptide nucleic acid acrocentric chromosome Alouatta animal cell article autosome centromere chromosome bivalent chromosome C band chromosome G band chromosome identification chromosome painting chromosome pairing chromosome structure diploidy DNA sequence female fluorescence in situ hybridization karyotype male meiosis metaphase mitosis morphology nonhuman nucleic acid probe priority journal telomere Animals Central America Female Karyotyping Male Meiosis Mitosis Platyrrhini Alouatta caraya Alouatta pigra Platyrrhini Primates |
| description |
We describe for the first time the karyotype of the black howler monkey, Alouatta pigra. Conventional staining, G- and C-banding, and fluorescence in situ hybridization (FISH) with a peptide nucleic acid (PNA) pantelomeric probe were performed. Eight free ranging adult individuals, four males and four females, within the natural distribution of the species presented a diploid karyotype with 2n = 58. Mitotic analyses showed an autosomal complement composed of 6 submetacentric, 3 metacentric, and 19 acrocentric chromosome pairs for females, and 6 submetacentric, 3 metacentric, and 18 acrocentric pairs for males. Meiotic analyses in males revealed 27 autosomal bivalents and a quadrivalent composed of a submetacentric X1 and acrocentric X 2, Y1, and Y2. The G-banded karyotype allowed us to identify pair #17 as the autosomal pair involved in the rearrangement and the morphology of the quadrivalent components. C-banding technique in metaphase I corroborated the structure of the quadrivalent showing four C+ centromeres. FISH analysis showed telomeric signals at the terminal regions of all chromosomes. No interstitial signals were detected. DNA sequence data were in accordance with those previously published for this species. Copyright © 2008 S. Karger AG. |
| format |
JOUR |
| author |
Steinberg, E.R. Cortés-Ortiz, L. Nieves, M. Bolzán, A.D. García-Orduña, F. Hermida-Lagunes, J. Canales-Espinosa, D. Mudry, M.D. |
| author_facet |
Steinberg, E.R. Cortés-Ortiz, L. Nieves, M. Bolzán, A.D. García-Orduña, F. Hermida-Lagunes, J. Canales-Espinosa, D. Mudry, M.D. |
| author_sort |
Steinberg, E.R. |
| title |
The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses |
| title_short |
The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses |
| title_full |
The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses |
| title_fullStr |
The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses |
| title_full_unstemmed |
The karyotype of Alouattapigra (Primates: Platyrrhini): Mitotic and meiotic analyses |
| title_sort |
karyotype of alouattapigra (primates: platyrrhini): mitotic and meiotic analyses |
| url |
http://hdl.handle.net/20.500.12110/paper_14248581_v122_n2_p103_Steinberg |
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