Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum

In this study we have performed a comparison of the most used spectroscopic glucose-oxidase assay for routine clinical monitoring of serum glucose, with the high-performance anion-exchange chromatographic method using pulsed amperometric detection (HPAEC-PAD). The linearity, repeatability and intern...

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Autores principales: Casabuono, A.C., Moirón, M.D.C., Buchta, C., Perego, M.D.C., Couto, A.S.
Formato: JOUR
Materias:
Glucose determination
Glucose-oxidase method
HPAEC-PAD
Human serum analysis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_14246376_v2005_n12_p243_Casabuono
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_14246376_v2005_n12_p243_Casabuono
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spelling todo:paper_14246376_v2005_n12_p243_Casabuono2023-10-03T16:13:41Z Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum Casabuono, A.C. Moirón, M.D.C. Buchta, C. Perego, M.D.C. Couto, A.S. Glucose determination Glucose-oxidase method HPAEC-PAD Human serum analysis In this study we have performed a comparison of the most used spectroscopic glucose-oxidase assay for routine clinical monitoring of serum glucose, with the high-performance anion-exchange chromatographic method using pulsed amperometric detection (HPAEC-PAD). The linearity, repeatability and internal reproducibility of the chromatographic method were determined. The correlation by linear regression and difference plot was analyzed. It is known that the enzymatic method leads to unreliable results when glucose must be determined in samples containing high concentration of bilirubin or lipids. On the contrary, the accuracy of the HPAEC-PAD method with these complex matrix sera resulted similar to normal samples showing no interferences. Another advantage was the minimal amount of human serum needed: one microliter was largely sufficient to perform the whole analysis. This fact would make the HPAEC-PAD method applicable when samples of preterm infants or new born babies of low weight must be analyzed. ©ARKAT. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14246376_v2005_n12_p243_Casabuono
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Glucose determination
Glucose-oxidase method
HPAEC-PAD
Human serum analysis
spellingShingle Glucose determination
Glucose-oxidase method
HPAEC-PAD
Human serum analysis
Casabuono, A.C.
Moirón, M.D.C.
Buchta, C.
Perego, M.D.C.
Couto, A.S.
Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
topic_facet Glucose determination
Glucose-oxidase method
HPAEC-PAD
Human serum analysis
description In this study we have performed a comparison of the most used spectroscopic glucose-oxidase assay for routine clinical monitoring of serum glucose, with the high-performance anion-exchange chromatographic method using pulsed amperometric detection (HPAEC-PAD). The linearity, repeatability and internal reproducibility of the chromatographic method were determined. The correlation by linear regression and difference plot was analyzed. It is known that the enzymatic method leads to unreliable results when glucose must be determined in samples containing high concentration of bilirubin or lipids. On the contrary, the accuracy of the HPAEC-PAD method with these complex matrix sera resulted similar to normal samples showing no interferences. Another advantage was the minimal amount of human serum needed: one microliter was largely sufficient to perform the whole analysis. This fact would make the HPAEC-PAD method applicable when samples of preterm infants or new born babies of low weight must be analyzed. ©ARKAT.
format JOUR
author Casabuono, A.C.
Moirón, M.D.C.
Buchta, C.
Perego, M.D.C.
Couto, A.S.
author_facet Casabuono, A.C.
Moirón, M.D.C.
Buchta, C.
Perego, M.D.C.
Couto, A.S.
author_sort Casabuono, A.C.
title Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
title_short Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
title_full Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
title_fullStr Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
title_full_unstemmed Comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
title_sort comparison of micro-enzymatic and high performance anion exchange chromatography methods for the analysis of glucose in human serum
url http://hdl.handle.net/20.500.12110/paper_14246376_v2005_n12_p243_Casabuono
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AT buchtac comparisonofmicroenzymaticandhighperformanceanionexchangechromatographymethodsfortheanalysisofglucoseinhumanserum
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