Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides

E. coli BL21 cells were able to synthesize several purine nucleosides from pyrimidine ones. Kinetics and yields of this reaction showed a strong dependence on pH, temperature, reagent concentrations and weight of wet cell paste. Yields over 90% were reached in the synthesis of adenosine.

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Detalles Bibliográficos
Autores principales: Rogert, M.C., Martínez, N., Porro, S., Lewkowicz, E., Iribarren, A.
Formato: JOUR
Materias:
purine nucleoside derivative
bacterial strain
biocatalyst
conference paper
drug synthesis
Escherichia coli
kinetics
nonhuman
pH measurement
quantum yield
temperature dependence
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_14203049_v5_n3_p535_Rogert
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:E. coli BL21 cells were able to synthesize several purine nucleosides from pyrimidine ones. Kinetics and yields of this reaction showed a strong dependence on pH, temperature, reagent concentrations and weight of wet cell paste. Yields over 90% were reached in the synthesis of adenosine.

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