Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides

E. coli BL21 cells were able to synthesize several purine nucleosides from pyrimidine ones. Kinetics and yields of this reaction showed a strong dependence on pH, temperature, reagent concentrations and weight of wet cell paste. Yields over 90% were reached in the synthesis of adenosine.

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Autores principales: Rogert, M.C., Martínez, N., Porro, S., Lewkowicz, E., Iribarren, A.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_14203049_v5_n3_p535_Rogert
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spelling todo:paper_14203049_v5_n3_p535_Rogert2023-10-03T16:13:24Z Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides Rogert, M.C. Martínez, N. Porro, S. Lewkowicz, E. Iribarren, A. purine nucleoside derivative bacterial strain biocatalyst conference paper drug synthesis Escherichia coli kinetics nonhuman pH measurement quantum yield temperature dependence E. coli BL21 cells were able to synthesize several purine nucleosides from pyrimidine ones. Kinetics and yields of this reaction showed a strong dependence on pH, temperature, reagent concentrations and weight of wet cell paste. Yields over 90% were reached in the synthesis of adenosine. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_14203049_v5_n3_p535_Rogert
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic purine nucleoside derivative
bacterial strain
biocatalyst
conference paper
drug synthesis
Escherichia coli
kinetics
nonhuman
pH measurement
quantum yield
temperature dependence
spellingShingle purine nucleoside derivative
bacterial strain
biocatalyst
conference paper
drug synthesis
Escherichia coli
kinetics
nonhuman
pH measurement
quantum yield
temperature dependence
Rogert, M.C.
Martínez, N.
Porro, S.
Lewkowicz, E.
Iribarren, A.
Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides
topic_facet purine nucleoside derivative
bacterial strain
biocatalyst
conference paper
drug synthesis
Escherichia coli
kinetics
nonhuman
pH measurement
quantum yield
temperature dependence
description E. coli BL21 cells were able to synthesize several purine nucleosides from pyrimidine ones. Kinetics and yields of this reaction showed a strong dependence on pH, temperature, reagent concentrations and weight of wet cell paste. Yields over 90% were reached in the synthesis of adenosine.
format JOUR
author Rogert, M.C.
Martínez, N.
Porro, S.
Lewkowicz, E.
Iribarren, A.
author_facet Rogert, M.C.
Martínez, N.
Porro, S.
Lewkowicz, E.
Iribarren, A.
author_sort Rogert, M.C.
title Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides
title_short Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides
title_full Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides
title_fullStr Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides
title_full_unstemmed Escherichia coli Bl21: A useful biocatalyst for the synthesis purine nucleosides
title_sort escherichia coli bl21: a useful biocatalyst for the synthesis purine nucleosides
url http://hdl.handle.net/20.500.12110/paper_14203049_v5_n3_p535_Rogert
work_keys_str_mv AT rogertmc escherichiacolibl21ausefulbiocatalystforthesynthesispurinenucleosides
AT martinezn escherichiacolibl21ausefulbiocatalystforthesynthesispurinenucleosides
AT porros escherichiacolibl21ausefulbiocatalystforthesynthesispurinenucleosides
AT lewkowicze escherichiacolibl21ausefulbiocatalystforthesynthesispurinenucleosides
AT iribarrena escherichiacolibl21ausefulbiocatalystforthesynthesispurinenucleosides
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