Cryopreservation procedure does not modify human carotid homografts mechanical properties: An isobaric and dynamic analysis
The viscoelastic and inertial properties of the arterial wall are responsible for the arterial functional role in the cardiovascular system. Cryopreservation is widely used to preserve blood vessels for vascular reconstruction but it is controversially suspected to affect the dynamic behaviour of th...
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| Lenguaje: | English |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_13899333_v7_n3_p183_Bia |
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todo:paper_13899333_v7_n3_p183_Bia2023-10-03T16:12:48Z Cryopreservation procedure does not modify human carotid homografts mechanical properties: An isobaric and dynamic analysis Bia, D. Pessana, F. Armentano, R. Pérez, H. Graf, S. Zócalo, Y. Saldías, M. Perez, N. Alvarez, O. Silva, W. Machin, D. Sueta, P. Ferrin, S. Acosta, M. Alvarez, I. Arterial wall Cryopreservation Human arteries Pressure-diameter Viscoelasticity nitrogen arterial pressure artery diameter artery graft artery wall article biomechanics blood vessel function common carotid artery controlled study cryopreservation gas graft preservation human human tissue priority journal pulse pressure stress thawing viscoelasticity Carotid Arteries Cryopreservation Elasticity Humans Transplantation, Homologous The viscoelastic and inertial properties of the arterial wall are responsible for the arterial functional role in the cardiovascular system. Cryopreservation is widely used to preserve blood vessels for vascular reconstruction but it is controversially suspected to affect the dynamic behaviour of these allografts. The aim of this work was to assess the cryopreservation's effects on human arteries mechanical properties. Common carotid artery (CCA) segments harvested from donors were divided into two groups: Fresh (n = 18), tested for 24-48 h after harvesting, and Cryopreserved (n = 18) for an average time of 30 days in gas-nitrogen phase, and finally defrosted. Each segment was tested in a circulation mock, and its pressure and diameter were registered at similar pump frequency, pulse and mean pressure levels, including those of normotensive and hipertensive conditions. A compliance transfer function (diameter/pressure) derived from a mathematical adaptive modelling was designed for the on line assessment of the arterial wall dynamics and its frequency response. Assessment of arterial wall dynamics was made by measuring its viscous (η), inertial (M) and elastic (E) properties, and creep and stress relaxation time constant (τC and τSR, respectively). The frequency response characterization allowed to evaluate the arterial wall filter or buffer function. Results showed that non-significant differences exist between wall dynamics and buffer function of fresh and cryopreserved segments of human CCA. In conclusion, our cryopreservation method maintains arterial wall functional properties, close to their fresh values. © Springer 2006. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13899333_v7_n3_p183_Bia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
English |
| orig_language_str_mv |
English |
| topic |
Arterial wall Cryopreservation Human arteries Pressure-diameter Viscoelasticity nitrogen arterial pressure artery diameter artery graft artery wall article biomechanics blood vessel function common carotid artery controlled study cryopreservation gas graft preservation human human tissue priority journal pulse pressure stress thawing viscoelasticity Carotid Arteries Cryopreservation Elasticity Humans Transplantation, Homologous |
| spellingShingle |
Arterial wall Cryopreservation Human arteries Pressure-diameter Viscoelasticity nitrogen arterial pressure artery diameter artery graft artery wall article biomechanics blood vessel function common carotid artery controlled study cryopreservation gas graft preservation human human tissue priority journal pulse pressure stress thawing viscoelasticity Carotid Arteries Cryopreservation Elasticity Humans Transplantation, Homologous Bia, D. Pessana, F. Armentano, R. Pérez, H. Graf, S. Zócalo, Y. Saldías, M. Perez, N. Alvarez, O. Silva, W. Machin, D. Sueta, P. Ferrin, S. Acosta, M. Alvarez, I. Cryopreservation procedure does not modify human carotid homografts mechanical properties: An isobaric and dynamic analysis |
| topic_facet |
Arterial wall Cryopreservation Human arteries Pressure-diameter Viscoelasticity nitrogen arterial pressure artery diameter artery graft artery wall article biomechanics blood vessel function common carotid artery controlled study cryopreservation gas graft preservation human human tissue priority journal pulse pressure stress thawing viscoelasticity Carotid Arteries Cryopreservation Elasticity Humans Transplantation, Homologous |
| description |
The viscoelastic and inertial properties of the arterial wall are responsible for the arterial functional role in the cardiovascular system. Cryopreservation is widely used to preserve blood vessels for vascular reconstruction but it is controversially suspected to affect the dynamic behaviour of these allografts. The aim of this work was to assess the cryopreservation's effects on human arteries mechanical properties. Common carotid artery (CCA) segments harvested from donors were divided into two groups: Fresh (n = 18), tested for 24-48 h after harvesting, and Cryopreserved (n = 18) for an average time of 30 days in gas-nitrogen phase, and finally defrosted. Each segment was tested in a circulation mock, and its pressure and diameter were registered at similar pump frequency, pulse and mean pressure levels, including those of normotensive and hipertensive conditions. A compliance transfer function (diameter/pressure) derived from a mathematical adaptive modelling was designed for the on line assessment of the arterial wall dynamics and its frequency response. Assessment of arterial wall dynamics was made by measuring its viscous (η), inertial (M) and elastic (E) properties, and creep and stress relaxation time constant (τC and τSR, respectively). The frequency response characterization allowed to evaluate the arterial wall filter or buffer function. Results showed that non-significant differences exist between wall dynamics and buffer function of fresh and cryopreserved segments of human CCA. In conclusion, our cryopreservation method maintains arterial wall functional properties, close to their fresh values. © Springer 2006. |
| format |
JOUR |
| author |
Bia, D. Pessana, F. Armentano, R. Pérez, H. Graf, S. Zócalo, Y. Saldías, M. Perez, N. Alvarez, O. Silva, W. Machin, D. Sueta, P. Ferrin, S. Acosta, M. Alvarez, I. |
| author_facet |
Bia, D. Pessana, F. Armentano, R. Pérez, H. Graf, S. Zócalo, Y. Saldías, M. Perez, N. Alvarez, O. Silva, W. Machin, D. Sueta, P. Ferrin, S. Acosta, M. Alvarez, I. |
| author_sort |
Bia, D. |
| title |
Cryopreservation procedure does not modify human carotid homografts mechanical properties: An isobaric and dynamic analysis |
| title_short |
Cryopreservation procedure does not modify human carotid homografts mechanical properties: An isobaric and dynamic analysis |
| title_full |
Cryopreservation procedure does not modify human carotid homografts mechanical properties: An isobaric and dynamic analysis |
| title_fullStr |
Cryopreservation procedure does not modify human carotid homografts mechanical properties: An isobaric and dynamic analysis |
| title_full_unstemmed |
Cryopreservation procedure does not modify human carotid homografts mechanical properties: An isobaric and dynamic analysis |
| title_sort |
cryopreservation procedure does not modify human carotid homografts mechanical properties: an isobaric and dynamic analysis |
| url |
http://hdl.handle.net/20.500.12110/paper_13899333_v7_n3_p183_Bia |
| work_keys_str_mv |
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