Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age

Several studies indicate that certain factors such as age, sex or nutritional status among others, may affect the level of DNA damage, both induced and spontaneous, so it is very important to consider them for a more accurate interpretation of the findings. The aim of this study was to analyze the i...

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Autores principales: Schaumburg, L.G., Poletta, G.L., Siroski, P.A., Mudry, M.D.
Formato: JOUR
Materias:
Age
Basal genetic damage
Comet assay
Micronucleus test
Monitoring
Tegu lizard
adolescent
adult
animal tissue
article
basal damage index
birth weight
blood analysis
comet assay
controlled study
erythrocyte
female
gender
genetic damage
genetic parameters
groups by age
lizard
male
micronucleus
newborn
nonhuman
priority journal
Tupinambis merianae
Animalia
Squamata
Tupinambis nigropunctatus
Aging
Animals
Erythroblasts
Humans
Lizards
Male
Micronuclei, Chromosome-Defective
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_13835718_v766_n_p5_Schaumburg
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_13835718_v766_n_p5_Schaumburg
record_format dspace
spelling todo:paper_13835718_v766_n_p5_Schaumburg2023-10-03T16:11:57Z Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age Schaumburg, L.G. Poletta, G.L. Siroski, P.A. Mudry, M.D. Age Basal genetic damage Comet assay Micronucleus test Monitoring Tegu lizard adolescent adult animal tissue article basal damage index birth weight blood analysis comet assay controlled study erythrocyte female gender genetic damage genetic parameters groups by age lizard male micronucleus newborn nonhuman priority journal Tupinambis merianae Animalia Squamata Tupinambis merianae Tupinambis nigropunctatus Age Basal genetic damage Comet assay Micronucleus test Monitoring Tegu lizard Aging Animals Erythroblasts Humans Lizards Male Micronuclei, Chromosome-Defective Several studies indicate that certain factors such as age, sex or nutritional status among others, may affect the level of DNA damage, both induced and spontaneous, so it is very important to consider them for a more accurate interpretation of the findings. The aim of this study was to analyze the influence of age, sex, and nest of origin on spontaneous genetic damage of Tupinambis merianae determined by the comet assay (CA) and the micronucleus (MN) test, in order to improve reference data for future in vivo studies of xenobiotics exposure in this species. Sixty-five tegu lizards of three different ages: newborns (NB), juveniles (JUV) and adults (AD), both sexes and from different nests of origin were used. Blood samples were collected from the caudal vein of all animals and the MN test and CA were applied on peripheral blood erythrocytes to determine basal frequency of MN (BFMN) and basal damage index (BDI). The comparison between age groups showed statistically significant differences in the BFMN and BDI (p<0.05). NB animals showed significantly higher BDI values in relation to JUV and AD (p<0.016), but no statistically differences were found between the latter two. NB showed lower BFMN respect to other age groups, being statistically significant only when compared to AD (p<0.016). BFMN or BDI showed no statistically significant differences between sexes or nests of origin (p>0.05). A weak negative relationship was found only between BFMN and weight of NB tegu lizard (p=0.014; R2=0.245). Basal values of genetic damage obtained with both biomarkers in the tegu lizard evidenced that age is an intrinsic factor that should be taken into account to avoid misunderstanding of the results in future biomonitoring studies. © 2014 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13835718_v766_n_p5_Schaumburg
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Age
Basal genetic damage
Comet assay
Micronucleus test
Monitoring
Tegu lizard
adolescent
adult
animal tissue
article
basal damage index
birth weight
blood analysis
comet assay
controlled study
erythrocyte
female
gender
genetic damage
genetic parameters
groups by age
lizard
male
micronucleus
newborn
nonhuman
priority journal
Tupinambis merianae
Animalia
Squamata
Tupinambis merianae
Tupinambis nigropunctatus
Age
Basal genetic damage
Comet assay
Micronucleus test
Monitoring
Tegu lizard
Aging
Animals
Erythroblasts
Humans
Lizards
Male
Micronuclei, Chromosome-Defective
spellingShingle Age
Basal genetic damage
Comet assay
Micronucleus test
Monitoring
Tegu lizard
adolescent
adult
animal tissue
article
basal damage index
birth weight
blood analysis
comet assay
controlled study
erythrocyte
female
gender
genetic damage
genetic parameters
groups by age
lizard
male
micronucleus
newborn
nonhuman
priority journal
Tupinambis merianae
Animalia
Squamata
Tupinambis merianae
Tupinambis nigropunctatus
Age
Basal genetic damage
Comet assay
Micronucleus test
Monitoring
Tegu lizard
Aging
Animals
Erythroblasts
Humans
Lizards
Male
Micronuclei, Chromosome-Defective
Schaumburg, L.G.
Poletta, G.L.
Siroski, P.A.
Mudry, M.D.
Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
topic_facet Age
Basal genetic damage
Comet assay
Micronucleus test
Monitoring
Tegu lizard
adolescent
adult
animal tissue
article
basal damage index
birth weight
blood analysis
comet assay
controlled study
erythrocyte
female
gender
genetic damage
genetic parameters
groups by age
lizard
male
micronucleus
newborn
nonhuman
priority journal
Tupinambis merianae
Animalia
Squamata
Tupinambis merianae
Tupinambis nigropunctatus
Age
Basal genetic damage
Comet assay
Micronucleus test
Monitoring
Tegu lizard
Aging
Animals
Erythroblasts
Humans
Lizards
Male
Micronuclei, Chromosome-Defective
description Several studies indicate that certain factors such as age, sex or nutritional status among others, may affect the level of DNA damage, both induced and spontaneous, so it is very important to consider them for a more accurate interpretation of the findings. The aim of this study was to analyze the influence of age, sex, and nest of origin on spontaneous genetic damage of Tupinambis merianae determined by the comet assay (CA) and the micronucleus (MN) test, in order to improve reference data for future in vivo studies of xenobiotics exposure in this species. Sixty-five tegu lizards of three different ages: newborns (NB), juveniles (JUV) and adults (AD), both sexes and from different nests of origin were used. Blood samples were collected from the caudal vein of all animals and the MN test and CA were applied on peripheral blood erythrocytes to determine basal frequency of MN (BFMN) and basal damage index (BDI). The comparison between age groups showed statistically significant differences in the BFMN and BDI (p<0.05). NB animals showed significantly higher BDI values in relation to JUV and AD (p<0.016), but no statistically differences were found between the latter two. NB showed lower BFMN respect to other age groups, being statistically significant only when compared to AD (p<0.016). BFMN or BDI showed no statistically significant differences between sexes or nests of origin (p>0.05). A weak negative relationship was found only between BFMN and weight of NB tegu lizard (p=0.014; R2=0.245). Basal values of genetic damage obtained with both biomarkers in the tegu lizard evidenced that age is an intrinsic factor that should be taken into account to avoid misunderstanding of the results in future biomonitoring studies. © 2014 Elsevier B.V.
format JOUR
author Schaumburg, L.G.
Poletta, G.L.
Siroski, P.A.
Mudry, M.D.
author_facet Schaumburg, L.G.
Poletta, G.L.
Siroski, P.A.
Mudry, M.D.
author_sort Schaumburg, L.G.
title Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
title_short Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
title_full Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
title_fullStr Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
title_full_unstemmed Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
title_sort spontaneous genetic damage in the tegu lizard (tupinambis merianae): the effect of age
url http://hdl.handle.net/20.500.12110/paper_13835718_v766_n_p5_Schaumburg
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AT siroskipa spontaneousgeneticdamageinthetegulizardtupinambismerianaetheeffectofage
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