Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L.
Zineb [ethylene bis(dithiocarbamate) zinc] is a widely employed foliar fungicide for agricultural and industrial applications. Allium cepa L. is a reliable model for the assessment of xenobiotic genotoxicity and cytotoxicity. We evaluated the effects of the zineb-containing commercial formulation Az...
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todo:paper_13835718_v742_n1-2_p48_Andrioli2023-10-03T16:11:56Z Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L. Andrioli, N.B. Soloneski, S. Larramendy, M.L. Mudry, M.D. Allium cepa Cytotoxicity Genotoxicity Microtubules Zineb zineb anaphase article cell cycle chromosome aberration concentration (parameters) controlled study cytogenetics genotoxicity micronucleus microtubule mitosis index mitosis spindle nonhuman onion phragmoplast plant cell plant root priority journal telophase Allium Cell Cycle Chromosome Aberrations Fungicides, Industrial Meristem Micronuclei, Chromosome-Defective Microtubules Mitotic Index Mitotic Spindle Apparatus Plant Roots Zineb Allium cepa Zineb [ethylene bis(dithiocarbamate) zinc] is a widely employed foliar fungicide for agricultural and industrial applications. Allium cepa L. is a reliable model for the assessment of xenobiotic genotoxicity and cytotoxicity. We evaluated the effects of the zineb-containing commercial formulation Azzurro ® (70% zineb) in cell cycle stages of the meristem root cells of A. cepa. The mitotic index (MI), chromosomal aberrations at anaphase/telophase (CAs), micronuclei (MN), and abnormalities in immunodetected microtubule structures, e.g., preprophasic band (PPB), mitotic spindle (MS), and phragmoplast (Phrag), were used as end-points. Azzurro ® (1 and 10μg/ml) induced a significant increase in the frequency of CAs (P<0.05), and the higher concentration inhibited the MI (P<0.05) compared to control values. The frequency of MN did not differ from control values at any concentration. Treatment with 1μg/ml Azzurro ® induced a significant increase in the frequency of abnormal PPB (P<0.01), MS (P<0.001), and Phrag (P<0.01) and, at 10μg/ml, enhancements in the frequencies of abnormal MS (P<0.05) and Phrag (P<0.05) were seen. A tubulin immunodetection assay showed that exposure to Azzurro ® interferes with normal assembly of microtubule structures during mitosis. © 2011 Elsevier B.V. Fil:Andrioli, N.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13835718_v742_n1-2_p48_Andrioli |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Allium cepa Cytotoxicity Genotoxicity Microtubules Zineb zineb anaphase article cell cycle chromosome aberration concentration (parameters) controlled study cytogenetics genotoxicity micronucleus microtubule mitosis index mitosis spindle nonhuman onion phragmoplast plant cell plant root priority journal telophase Allium Cell Cycle Chromosome Aberrations Fungicides, Industrial Meristem Micronuclei, Chromosome-Defective Microtubules Mitotic Index Mitotic Spindle Apparatus Plant Roots Zineb Allium cepa |
| spellingShingle |
Allium cepa Cytotoxicity Genotoxicity Microtubules Zineb zineb anaphase article cell cycle chromosome aberration concentration (parameters) controlled study cytogenetics genotoxicity micronucleus microtubule mitosis index mitosis spindle nonhuman onion phragmoplast plant cell plant root priority journal telophase Allium Cell Cycle Chromosome Aberrations Fungicides, Industrial Meristem Micronuclei, Chromosome-Defective Microtubules Mitotic Index Mitotic Spindle Apparatus Plant Roots Zineb Allium cepa Andrioli, N.B. Soloneski, S. Larramendy, M.L. Mudry, M.D. Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L. |
| topic_facet |
Allium cepa Cytotoxicity Genotoxicity Microtubules Zineb zineb anaphase article cell cycle chromosome aberration concentration (parameters) controlled study cytogenetics genotoxicity micronucleus microtubule mitosis index mitosis spindle nonhuman onion phragmoplast plant cell plant root priority journal telophase Allium Cell Cycle Chromosome Aberrations Fungicides, Industrial Meristem Micronuclei, Chromosome-Defective Microtubules Mitotic Index Mitotic Spindle Apparatus Plant Roots Zineb Allium cepa |
| description |
Zineb [ethylene bis(dithiocarbamate) zinc] is a widely employed foliar fungicide for agricultural and industrial applications. Allium cepa L. is a reliable model for the assessment of xenobiotic genotoxicity and cytotoxicity. We evaluated the effects of the zineb-containing commercial formulation Azzurro ® (70% zineb) in cell cycle stages of the meristem root cells of A. cepa. The mitotic index (MI), chromosomal aberrations at anaphase/telophase (CAs), micronuclei (MN), and abnormalities in immunodetected microtubule structures, e.g., preprophasic band (PPB), mitotic spindle (MS), and phragmoplast (Phrag), were used as end-points. Azzurro ® (1 and 10μg/ml) induced a significant increase in the frequency of CAs (P<0.05), and the higher concentration inhibited the MI (P<0.05) compared to control values. The frequency of MN did not differ from control values at any concentration. Treatment with 1μg/ml Azzurro ® induced a significant increase in the frequency of abnormal PPB (P<0.01), MS (P<0.001), and Phrag (P<0.01) and, at 10μg/ml, enhancements in the frequencies of abnormal MS (P<0.05) and Phrag (P<0.05) were seen. A tubulin immunodetection assay showed that exposure to Azzurro ® interferes with normal assembly of microtubule structures during mitosis. © 2011 Elsevier B.V. |
| format |
JOUR |
| author |
Andrioli, N.B. Soloneski, S. Larramendy, M.L. Mudry, M.D. |
| author_facet |
Andrioli, N.B. Soloneski, S. Larramendy, M.L. Mudry, M.D. |
| author_sort |
Andrioli, N.B. |
| title |
Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L. |
| title_short |
Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L. |
| title_full |
Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L. |
| title_fullStr |
Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L. |
| title_full_unstemmed |
Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro ® on meristematic root cells of Allium cepa L. |
| title_sort |
cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation azzurro ® on meristematic root cells of allium cepa l. |
| url |
http://hdl.handle.net/20.500.12110/paper_13835718_v742_n1-2_p48_Andrioli |
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