Imaging molecular interactions in living cells by FRET microscopy

Förster resonance energy transfer (FRET) is applied extensively in all fields of biological research and technology, generally as a 'nanoruler' with a dynamic range corresponding to the intramolecular and intermolecular distances characterizing the molecular structures that regulate cellul...

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Autores principales: Jares-Erijman, E.A., Jovin, T.M.
Formato: JOUR
Materias:
cell function
conformational transition
fluorescence resonance energy transfer
molecular interaction
protein modification
protein transport
review
Cells
Fluorescence Resonance Energy Transfer
Proteins
Quantum Dots
Quantum Theory
Sensitivity and Specificity
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_13675931_v10_n5_p409_JaresErijman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_13675931_v10_n5_p409_JaresErijman
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spelling todo:paper_13675931_v10_n5_p409_JaresErijman2023-10-03T16:11:35Z Imaging molecular interactions in living cells by FRET microscopy Jares-Erijman, E.A. Jovin, T.M. cell function conformational transition fluorescence resonance energy transfer molecular interaction protein modification protein transport review Cells Fluorescence Resonance Energy Transfer Proteins Quantum Dots Quantum Theory Sensitivity and Specificity Förster resonance energy transfer (FRET) is applied extensively in all fields of biological research and technology, generally as a 'nanoruler' with a dynamic range corresponding to the intramolecular and intermolecular distances characterizing the molecular structures that regulate cellular function. The complex underlying network of interactions reflects elementary reactions operating under strict spatio-temporal control: binding, conformational transition, covalent modification and transport. FRET imaging provides information about all these molecular processes with high specificity and sensitivity via probes expressed by or introduced from the external medium into the cell, tissue or organism. Current approaches and developments in the field are discussed with emphasis on formalism, probes and technical implementation. © 2006 Elsevier Ltd. All rights reserved. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13675931_v10_n5_p409_JaresErijman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cell function
conformational transition
fluorescence resonance energy transfer
molecular interaction
protein modification
protein transport
review
Cells
Fluorescence Resonance Energy Transfer
Proteins
Quantum Dots
Quantum Theory
Sensitivity and Specificity
spellingShingle cell function
conformational transition
fluorescence resonance energy transfer
molecular interaction
protein modification
protein transport
review
Cells
Fluorescence Resonance Energy Transfer
Proteins
Quantum Dots
Quantum Theory
Sensitivity and Specificity
Jares-Erijman, E.A.
Jovin, T.M.
Imaging molecular interactions in living cells by FRET microscopy
topic_facet cell function
conformational transition
fluorescence resonance energy transfer
molecular interaction
protein modification
protein transport
review
Cells
Fluorescence Resonance Energy Transfer
Proteins
Quantum Dots
Quantum Theory
Sensitivity and Specificity
description Förster resonance energy transfer (FRET) is applied extensively in all fields of biological research and technology, generally as a 'nanoruler' with a dynamic range corresponding to the intramolecular and intermolecular distances characterizing the molecular structures that regulate cellular function. The complex underlying network of interactions reflects elementary reactions operating under strict spatio-temporal control: binding, conformational transition, covalent modification and transport. FRET imaging provides information about all these molecular processes with high specificity and sensitivity via probes expressed by or introduced from the external medium into the cell, tissue or organism. Current approaches and developments in the field are discussed with emphasis on formalism, probes and technical implementation. © 2006 Elsevier Ltd. All rights reserved.
format JOUR
author Jares-Erijman, E.A.
Jovin, T.M.
author_facet Jares-Erijman, E.A.
Jovin, T.M.
author_sort Jares-Erijman, E.A.
title Imaging molecular interactions in living cells by FRET microscopy
title_short Imaging molecular interactions in living cells by FRET microscopy
title_full Imaging molecular interactions in living cells by FRET microscopy
title_fullStr Imaging molecular interactions in living cells by FRET microscopy
title_full_unstemmed Imaging molecular interactions in living cells by FRET microscopy
title_sort imaging molecular interactions in living cells by fret microscopy
url http://hdl.handle.net/20.500.12110/paper_13675931_v10_n5_p409_JaresErijman
work_keys_str_mv AT jareserijmanea imagingmolecularinteractionsinlivingcellsbyfretmicroscopy
AT jovintm imagingmolecularinteractionsinlivingcellsbyfretmicroscopy
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