Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach
Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; however, a dy...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_13675435_v45_n1_p15_Tribelli |
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todo:paper_13675435_v45_n1_p15_Tribelli2023-10-03T16:11:35Z Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach Tribelli, P.M. Rossi, L. Ricardi, M.M. Gomez-Lozano, M. Molin, S. Raiger Iustman, L.J. Lopez, N.I. Alkane degradation alkB Micro-aerobiosis Pseudomonas extremaustralis RNA-seq alcohol dehydrogenase aldehyde dehydrogenase alkane alkane 1 monooxygenase diesel fuel hydrocarbon oxidoreductase oxygen RNA rubredoxin rubredoxine reductase unclassified drug aerobic metabolism amino acid metabolism argc gene Article azu gene bacterial count carbon source cell viability controlled study degradation denitrification down regulation flga gene gene gene expression genetic organization nonhuman promoter region Pseudomonas Pseudomonas extremaustralis reverse transcription polymerase chain reaction RNA sequence transcriptomics upregulation Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; however, a dynamic spectrum of oxygen availability can be found in the environment. Pseudomonas extremaustralis, an Antarctic bacterium isolated from a pristine environment, is able to degrade diesel fuel and presents a wide microaerophilic metabolism. In this work RNA-deep sequence experiments were analyzed comparing the expression profile in aerobic and microaerophilic cultures. Interestingly, genes involved in alkane degradation, including alkB, were over-expressed in micro-aerobiosis in absence of hydrocarbon compounds. In minimal media supplemented with diesel fuel, n-alkanes degradation (C13–C19) after 7 days was observed under low oxygen conditions but not in aerobiosis. In-silico analysis of the alkB promoter zone showed a putative binding sequence for the anaerobic global regulator, Anr. Our results indicate that some diesel fuel components can be utilized as sole carbon source under microaerophilic conditions for cell maintenance or slow growth in a Pseudomonas species and this metabolism could represent an adaptive advantage in polluted environments. © 2017, Society for Industrial Microbiology and Biotechnology. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13675435_v45_n1_p15_Tribelli |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alkane degradation alkB Micro-aerobiosis Pseudomonas extremaustralis RNA-seq alcohol dehydrogenase aldehyde dehydrogenase alkane alkane 1 monooxygenase diesel fuel hydrocarbon oxidoreductase oxygen RNA rubredoxin rubredoxine reductase unclassified drug aerobic metabolism amino acid metabolism argc gene Article azu gene bacterial count carbon source cell viability controlled study degradation denitrification down regulation flga gene gene gene expression genetic organization nonhuman promoter region Pseudomonas Pseudomonas extremaustralis reverse transcription polymerase chain reaction RNA sequence transcriptomics upregulation |
| spellingShingle |
Alkane degradation alkB Micro-aerobiosis Pseudomonas extremaustralis RNA-seq alcohol dehydrogenase aldehyde dehydrogenase alkane alkane 1 monooxygenase diesel fuel hydrocarbon oxidoreductase oxygen RNA rubredoxin rubredoxine reductase unclassified drug aerobic metabolism amino acid metabolism argc gene Article azu gene bacterial count carbon source cell viability controlled study degradation denitrification down regulation flga gene gene gene expression genetic organization nonhuman promoter region Pseudomonas Pseudomonas extremaustralis reverse transcription polymerase chain reaction RNA sequence transcriptomics upregulation Tribelli, P.M. Rossi, L. Ricardi, M.M. Gomez-Lozano, M. Molin, S. Raiger Iustman, L.J. Lopez, N.I. Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
| topic_facet |
Alkane degradation alkB Micro-aerobiosis Pseudomonas extremaustralis RNA-seq alcohol dehydrogenase aldehyde dehydrogenase alkane alkane 1 monooxygenase diesel fuel hydrocarbon oxidoreductase oxygen RNA rubredoxin rubredoxine reductase unclassified drug aerobic metabolism amino acid metabolism argc gene Article azu gene bacterial count carbon source cell viability controlled study degradation denitrification down regulation flga gene gene gene expression genetic organization nonhuman promoter region Pseudomonas Pseudomonas extremaustralis reverse transcription polymerase chain reaction RNA sequence transcriptomics upregulation |
| description |
Diesel fuel is one of the most important sources of hydrocarbon contamination worldwide. Its composition consists of a complex mixture of n-alkanes, branched alkanes and aromatic compounds. Hydrocarbon degradation in Pseudomonas species has been mostly studied under aerobic conditions; however, a dynamic spectrum of oxygen availability can be found in the environment. Pseudomonas extremaustralis, an Antarctic bacterium isolated from a pristine environment, is able to degrade diesel fuel and presents a wide microaerophilic metabolism. In this work RNA-deep sequence experiments were analyzed comparing the expression profile in aerobic and microaerophilic cultures. Interestingly, genes involved in alkane degradation, including alkB, were over-expressed in micro-aerobiosis in absence of hydrocarbon compounds. In minimal media supplemented with diesel fuel, n-alkanes degradation (C13–C19) after 7 days was observed under low oxygen conditions but not in aerobiosis. In-silico analysis of the alkB promoter zone showed a putative binding sequence for the anaerobic global regulator, Anr. Our results indicate that some diesel fuel components can be utilized as sole carbon source under microaerophilic conditions for cell maintenance or slow growth in a Pseudomonas species and this metabolism could represent an adaptive advantage in polluted environments. © 2017, Society for Industrial Microbiology and Biotechnology. |
| format |
JOUR |
| author |
Tribelli, P.M. Rossi, L. Ricardi, M.M. Gomez-Lozano, M. Molin, S. Raiger Iustman, L.J. Lopez, N.I. |
| author_facet |
Tribelli, P.M. Rossi, L. Ricardi, M.M. Gomez-Lozano, M. Molin, S. Raiger Iustman, L.J. Lopez, N.I. |
| author_sort |
Tribelli, P.M. |
| title |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
| title_short |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
| title_full |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
| title_fullStr |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
| title_full_unstemmed |
Microaerophilic alkane degradation in Pseudomonas extremaustralis: a transcriptomic and physiological approach |
| title_sort |
microaerophilic alkane degradation in pseudomonas extremaustralis: a transcriptomic and physiological approach |
| url |
http://hdl.handle.net/20.500.12110/paper_13675435_v45_n1_p15_Tribelli |
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