Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke

Fomes sclerodermeus produces manganese peroxidase (MnP) and laccase as part of its ligninolytic system. A Doehlert experimental design was applied in order to find the optimum conditions for MnP and laccase production. The factors studied were Cu2+, Mn2+ and asparagine. The present model and data an...

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Autores principales:	Papinutti, V.L., Forchiassin, F.
Formato:	JOUR
Materias:	Fomes sclerodermeus Laccase Manganese peroxidase Statistical experimental design asparagine copper ion copper sulfate laccase manganese manganese peroxidase manganese sulfate article controlled study enzyme synthesis experimental design Fomes scleroderneus fungus fungus culture fungus growth model nonhuman nutrient process optimization South America Asparagine Biomass Biotechnology Copper Culture Media Fungi Lignin Manganese Models, Biological Peroxidases Fomes
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13675435_v30_n9_p536_Papinutti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_13675435_v30_n9_p536_Papinutti
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spelling	todo:paper_13675435_v30_n9_p536_Papinutti2023-10-03T16:11:35Z Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke Papinutti, V.L. Forchiassin, F. Fomes sclerodermeus Laccase Manganese peroxidase Statistical experimental design asparagine copper ion copper sulfate laccase manganese manganese peroxidase manganese sulfate article controlled study enzyme synthesis experimental design Fomes scleroderneus fungus fungus culture fungus growth model nonhuman nutrient process optimization South America Asparagine Biomass Biotechnology Copper Culture Media Fungi Laccase Lignin Manganese Models, Biological Peroxidases South America Fomes Fungi Fomes sclerodermeus produces manganese peroxidase (MnP) and laccase as part of its ligninolytic system. A Doehlert experimental design was applied in order to find the optimum conditions for MnP and laccase production. The factors studied were Cu2+, Mn2+ and asparagine. The present model and data analysis allowed us not only to define optimal media for production of both laccase and MnP, but also to show the combined effects between the factors. MnP was strongly influenced by Mn2+, which acts as an inducer. Under these conditions Cu2+ negatively affected MnP activity. At 13 days of growth 0.75 U ml-1 were produced in the optimized culture medium supplemented with 1 mM MnSO4 and 4 g l -1 asparagine. The laccase titer under optimized conditions reached maximum values at 16 days of growth: 13.5 U ml-1 in the presence of 0.2 mM CuSO4, 0.4 mM MnSO4 and 6 g l-1 asparagine. Mn2+ promoted production of both enzymes. There were important interactions among the nutrients evaluated, the most significant being those between Cu2+ and asparagine. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13675435_v30_n9_p536_Papinutti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Fomes sclerodermeus Laccase Manganese peroxidase Statistical experimental design asparagine copper ion copper sulfate laccase manganese manganese peroxidase manganese sulfate article controlled study enzyme synthesis experimental design Fomes scleroderneus fungus fungus culture fungus growth model nonhuman nutrient process optimization South America Asparagine Biomass Biotechnology Copper Culture Media Fungi Laccase Lignin Manganese Models, Biological Peroxidases South America Fomes Fungi
spellingShingle	Fomes sclerodermeus Laccase Manganese peroxidase Statistical experimental design asparagine copper ion copper sulfate laccase manganese manganese peroxidase manganese sulfate article controlled study enzyme synthesis experimental design Fomes scleroderneus fungus fungus culture fungus growth model nonhuman nutrient process optimization South America Asparagine Biomass Biotechnology Copper Culture Media Fungi Laccase Lignin Manganese Models, Biological Peroxidases South America Fomes Fungi Papinutti, V.L. Forchiassin, F. Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke
topic_facet	Fomes sclerodermeus Laccase Manganese peroxidase Statistical experimental design asparagine copper ion copper sulfate laccase manganese manganese peroxidase manganese sulfate article controlled study enzyme synthesis experimental design Fomes scleroderneus fungus fungus culture fungus growth model nonhuman nutrient process optimization South America Asparagine Biomass Biotechnology Copper Culture Media Fungi Laccase Lignin Manganese Models, Biological Peroxidases South America Fomes Fungi
description	Fomes sclerodermeus produces manganese peroxidase (MnP) and laccase as part of its ligninolytic system. A Doehlert experimental design was applied in order to find the optimum conditions for MnP and laccase production. The factors studied were Cu2+, Mn2+ and asparagine. The present model and data analysis allowed us not only to define optimal media for production of both laccase and MnP, but also to show the combined effects between the factors. MnP was strongly influenced by Mn2+, which acts as an inducer. Under these conditions Cu2+ negatively affected MnP activity. At 13 days of growth 0.75 U ml-1 were produced in the optimized culture medium supplemented with 1 mM MnSO4 and 4 g l -1 asparagine. The laccase titer under optimized conditions reached maximum values at 16 days of growth: 13.5 U ml-1 in the presence of 0.2 mM CuSO4, 0.4 mM MnSO4 and 6 g l-1 asparagine. Mn2+ promoted production of both enzymes. There were important interactions among the nutrients evaluated, the most significant being those between Cu2+ and asparagine.
format	JOUR
author	Papinutti, V.L. Forchiassin, F.
author_facet	Papinutti, V.L. Forchiassin, F.
author_sort	Papinutti, V.L.
title	Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke
title_short	Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke
title_full	Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke
title_fullStr	Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke
title_full_unstemmed	Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke
title_sort	optimization of manganese peroxidase and laccase production in the south american fungus fomes sclerodermeus (lév.) cke
url	http://hdl.handle.net/20.500.12110/paper_13675435_v30_n9_p536_Papinutti
work_keys_str_mv	AT papinuttivl optimizationofmanganeseperoxidaseandlaccaseproductioninthesouthamericanfungusfomessclerodermeuslevcke AT forchiassinf optimizationofmanganeseperoxidaseandlaccaseproductioninthesouthamericanfungusfomessclerodermeuslevcke
_version_	1782024269566312448

Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lév.) Cke

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