Clonostachys rosea BAFC3874 as a Sclerotinia sclerotiorum antagonist: Mechanisms involved and potential as a biocontrol agent
Aims: To establish the modes of action of the antagonistic fungal strain Clonostachys rosea BAFC3874 isolated from suppressive soils against Sclerotinia sclerotiorum and to determine its potential as a biocontrol agent. Methods and Results: The antagonistic activity of C. rosea BAFC3874 was determin...
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todo:paper_13645072_v110_n5_p1177_Rodriguez2023-10-03T16:11:01Z Clonostachys rosea BAFC3874 as a Sclerotinia sclerotiorum antagonist: Mechanisms involved and potential as a biocontrol agent Rodriguez, M.A. Cabrera, G. Gozzo, F.C. Eberlin, M.N. Godeas, A. Antagonism Biocontrol Clonostachys rosea Peptaibols Sclerotinia sclerotiorum peptaibol peptide derivative antagonism antimicrobial activity bioassay biocontrol agent biological control experimental study fungus growth response inhibition metabolite parasitism peptide soil microorganism soybean antifungal activity article biological control agent Clonostachys rosea bafc3874 controlled study fungal strain fungus growth growth inhibition mycoparasitism nonhuman peptide synthesis Sclerotinia sclerotiorum Antibiosis Ascomycota Hypocreales Lettuce Mycelium Plant Diseases Soil Microbiology Soybeans Bionectria ochroleuca Fungi Glycine max Lactuca Sclerotinia sclerotiorum Aims: To establish the modes of action of the antagonistic fungal strain Clonostachys rosea BAFC3874 isolated from suppressive soils against Sclerotinia sclerotiorum and to determine its potential as a biocontrol agent. Methods and Results: The antagonistic activity of C. rosea BAFC3874 was determined in vitro by dual cultures. The strain effectively antagonized S. sclerotiorum in pot-grown lettuce and soybean plants. Antifungal activity assays of C. rosea BAFC3874 grown in culture established that the strain produced antifungal compounds against S. sclerotiorum associated with secondary metabolism. High mycelial growth inhibition coincided with sclerotia production inhibition. The C. rosea strain produced a microheterogeneous mixture of peptides belonging to the peptaibiotic family. Moreover, mycoparasitism activity was observed in the dual culture. Conclusions: Clonostachys rosea strain BAFC3874 was proved to be an effective antagonist against the aggressive soil-borne pathogen S. sclerotiorum in greenhouse experiments. The main mechanisms involve peptaibiotic metabolite production and mycoparasitism activity. Significance and Impact of the Study: Clonostachys rosea BAFC3874 may be a good fungal biological control agent against S. sclerotiorum. In addition, we were also able to isolate and identify peptaibols, an unusual family of compounds in this genus of fungi. © 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology. Fil:Rodriguez, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cabrera, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Godeas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13645072_v110_n5_p1177_Rodriguez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antagonism Biocontrol Clonostachys rosea Peptaibols Sclerotinia sclerotiorum peptaibol peptide derivative antagonism antimicrobial activity bioassay biocontrol agent biological control experimental study fungus growth response inhibition metabolite parasitism peptide soil microorganism soybean antifungal activity article biological control agent Clonostachys rosea bafc3874 controlled study fungal strain fungus growth growth inhibition mycoparasitism nonhuman peptide synthesis Sclerotinia sclerotiorum Antibiosis Ascomycota Hypocreales Lettuce Mycelium Plant Diseases Soil Microbiology Soybeans Bionectria ochroleuca Fungi Glycine max Lactuca Sclerotinia sclerotiorum |
| spellingShingle |
Antagonism Biocontrol Clonostachys rosea Peptaibols Sclerotinia sclerotiorum peptaibol peptide derivative antagonism antimicrobial activity bioassay biocontrol agent biological control experimental study fungus growth response inhibition metabolite parasitism peptide soil microorganism soybean antifungal activity article biological control agent Clonostachys rosea bafc3874 controlled study fungal strain fungus growth growth inhibition mycoparasitism nonhuman peptide synthesis Sclerotinia sclerotiorum Antibiosis Ascomycota Hypocreales Lettuce Mycelium Plant Diseases Soil Microbiology Soybeans Bionectria ochroleuca Fungi Glycine max Lactuca Sclerotinia sclerotiorum Rodriguez, M.A. Cabrera, G. Gozzo, F.C. Eberlin, M.N. Godeas, A. Clonostachys rosea BAFC3874 as a Sclerotinia sclerotiorum antagonist: Mechanisms involved and potential as a biocontrol agent |
| topic_facet |
Antagonism Biocontrol Clonostachys rosea Peptaibols Sclerotinia sclerotiorum peptaibol peptide derivative antagonism antimicrobial activity bioassay biocontrol agent biological control experimental study fungus growth response inhibition metabolite parasitism peptide soil microorganism soybean antifungal activity article biological control agent Clonostachys rosea bafc3874 controlled study fungal strain fungus growth growth inhibition mycoparasitism nonhuman peptide synthesis Sclerotinia sclerotiorum Antibiosis Ascomycota Hypocreales Lettuce Mycelium Plant Diseases Soil Microbiology Soybeans Bionectria ochroleuca Fungi Glycine max Lactuca Sclerotinia sclerotiorum |
| description |
Aims: To establish the modes of action of the antagonistic fungal strain Clonostachys rosea BAFC3874 isolated from suppressive soils against Sclerotinia sclerotiorum and to determine its potential as a biocontrol agent. Methods and Results: The antagonistic activity of C. rosea BAFC3874 was determined in vitro by dual cultures. The strain effectively antagonized S. sclerotiorum in pot-grown lettuce and soybean plants. Antifungal activity assays of C. rosea BAFC3874 grown in culture established that the strain produced antifungal compounds against S. sclerotiorum associated with secondary metabolism. High mycelial growth inhibition coincided with sclerotia production inhibition. The C. rosea strain produced a microheterogeneous mixture of peptides belonging to the peptaibiotic family. Moreover, mycoparasitism activity was observed in the dual culture. Conclusions: Clonostachys rosea strain BAFC3874 was proved to be an effective antagonist against the aggressive soil-borne pathogen S. sclerotiorum in greenhouse experiments. The main mechanisms involve peptaibiotic metabolite production and mycoparasitism activity. Significance and Impact of the Study: Clonostachys rosea BAFC3874 may be a good fungal biological control agent against S. sclerotiorum. In addition, we were also able to isolate and identify peptaibols, an unusual family of compounds in this genus of fungi. © 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology. |
| format |
JOUR |
| author |
Rodriguez, M.A. Cabrera, G. Gozzo, F.C. Eberlin, M.N. Godeas, A. |
| author_facet |
Rodriguez, M.A. Cabrera, G. Gozzo, F.C. Eberlin, M.N. Godeas, A. |
| author_sort |
Rodriguez, M.A. |
| title |
Clonostachys rosea BAFC3874 as a Sclerotinia sclerotiorum antagonist: Mechanisms involved and potential as a biocontrol agent |
| title_short |
Clonostachys rosea BAFC3874 as a Sclerotinia sclerotiorum antagonist: Mechanisms involved and potential as a biocontrol agent |
| title_full |
Clonostachys rosea BAFC3874 as a Sclerotinia sclerotiorum antagonist: Mechanisms involved and potential as a biocontrol agent |
| title_fullStr |
Clonostachys rosea BAFC3874 as a Sclerotinia sclerotiorum antagonist: Mechanisms involved and potential as a biocontrol agent |
| title_full_unstemmed |
Clonostachys rosea BAFC3874 as a Sclerotinia sclerotiorum antagonist: Mechanisms involved and potential as a biocontrol agent |
| title_sort |
clonostachys rosea bafc3874 as a sclerotinia sclerotiorum antagonist: mechanisms involved and potential as a biocontrol agent |
| url |
http://hdl.handle.net/20.500.12110/paper_13645072_v110_n5_p1177_Rodriguez |
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