Coupling of tyrosine deprotonation and axial ligand exchange in nitrocytochrome c

Here we report a spectroscopic, electrochemical and computational study of cytochrome c showing that nitration of Tyr74 induces Tyr deprotonation, which is coupled to Met/Lys axial ligand exchange, and results in concomitant gain of peroxidatic activity at physiological pH. © The Royal Society of Ch...

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Detalles Bibliográficos
Autores principales: Capdevila, D.A., Álvarez-Paggi, D., Castro, M.A., Tórtora, V., Demicheli, V., Estrín, D.A., Radi, R., Murgida, D.H.
Formato: JOUR
Materias:
cytochrome c
ligand
tyrosine
article
computational fluid dynamics
computer model
controlled study
cyclic potentiometry
deprotonation
electrochemistry
electron transport
molecular dynamics
nitration
nuclear magnetic resonance spectroscopy
pH
proton transport
Computer Simulation
Cytochromes c
Hydrogen-Ion Concentration
Ligands
Models, Molecular
Nitrates
Oxidation-Reduction
Protons
Thermodynamics
Tyrosine
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_13597345_v50_n20_p2592_Capdevila
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Here we report a spectroscopic, electrochemical and computational study of cytochrome c showing that nitration of Tyr74 induces Tyr deprotonation, which is coupled to Met/Lys axial ligand exchange, and results in concomitant gain of peroxidatic activity at physiological pH. © The Royal Society of Chemistry 2014.

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