RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells

The recently cloned small RWD-domain containing protein RSUME was shown to increase protein levels of hypoxia-inducible factor-1α (HIF-1α). The latter is the oxygen-regulated subunit of HIF-1, the most important transcription factor of the cellular adaptive processes to hypoxic conditions. It is als...

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Autores principales:	Shan, B., Gerez, J., Haedo, M., Fuertes, M., Theodoropoulou, M., Buchfelder, M., Losa, M., Stalla, G.K., Arzt, E., Renner, U.
Formato:	JOUR
Materias:	cobalt chloride hypoxia inducible factor 1alpha messenger RNA protein protein RSUME small interfering RNA unclassified drug vasculotropin A cobalt HIF1A protein, human RSUME protein, human transcription factor VEGFA protein, human article cancer cell culture cell nucleus controlled study correlation analysis down regulation human human cell human tissue hypophysis adenoma hypoxia protein expression protein function protein induction protein secretion protein transport RNA interference upregulation adenoma animal anoxia biosynthesis genetics hypophysis tumor metabolism mouse neovascularization (pathology) tumor cell culture tumor cell line Adenoma Animals Anoxia Cell Line, Tumor Cobalt Humans Hypoxia-Inducible Factor 1, alpha Subunit Mice Neovascularization, Pathologic Pituitary Neoplasms RNA, Messenger Transcription Factors Tumor Cells, Cultured Vascular Endothelial Growth Factor A
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13510088_v19_n1_p13_Shan
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_13510088_v19_n1_p13_Shan
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spelling	todo:paper_13510088_v19_n1_p13_Shan2023-10-03T16:10:07Z RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells Shan, B. Gerez, J. Haedo, M. Fuertes, M. Theodoropoulou, M. Buchfelder, M. Losa, M. Stalla, G.K. Arzt, E. Renner, U. cobalt chloride hypoxia inducible factor 1alpha messenger RNA protein protein RSUME small interfering RNA unclassified drug vasculotropin A cobalt HIF1A protein, human hypoxia inducible factor 1alpha messenger RNA RSUME protein, human transcription factor vasculotropin A VEGFA protein, human article cancer cell culture cell nucleus controlled study correlation analysis down regulation human human cell human tissue hypophysis adenoma hypoxia protein expression protein function protein induction protein secretion protein transport RNA interference upregulation adenoma animal anoxia biosynthesis genetics hypophysis tumor metabolism mouse neovascularization (pathology) tumor cell culture tumor cell line Adenoma Animals Anoxia Cell Line, Tumor Cobalt Humans Hypoxia-Inducible Factor 1, alpha Subunit Mice Neovascularization, Pathologic Pituitary Neoplasms RNA, Messenger Transcription Factors Tumor Cells, Cultured Vascular Endothelial Growth Factor A The recently cloned small RWD-domain containing protein RSUME was shown to increase protein levels of hypoxia-inducible factor-1α (HIF-1α). The latter is the oxygen-regulated subunit of HIF-1, the most important transcription factor of the cellular adaptive processes to hypoxic conditions. It is also a major regulator of vascular endothelial growth factor-A (VEGF-A), which is critically involved in the complex process of tumour neovascularisation. In this study, the expression and role of RSUME in pituitary tumours was studied. We found that RSUME mRNA was up-regulated in pituitary adenomas and significantly correlated with HIF-1α mRNA levels. Hypoxia (1% O 2) or treatment with hypoxia-mimicking CoCl 2 enhanced RSUME and HIF-1α expression, induced translocation of HIF-1α to the nuclei and stimulated VEGF-A production both in pituitary tumour cell lines and primary human pituitary adenoma cell cultures. When RSUME expression was specifically down-regulated by siRNA, the CoCl 2-induced increase VEGF-A secretion was strongly reduced which was shown to be a consequence of the RSUME knockdown-associated reduction of HIF-1α synthesis. Thus, RSUME plays an important role in initiating pituitary tumour neovascularisation through regulating HIF-1α levels and subsequent VEGF-A production and may therefore be critically involved in pituitary adenoma progression. © 2012 Society for Endocrinology Printed in Great Britain. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13510088_v19_n1_p13_Shan
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cobalt chloride hypoxia inducible factor 1alpha messenger RNA protein protein RSUME small interfering RNA unclassified drug vasculotropin A cobalt HIF1A protein, human hypoxia inducible factor 1alpha messenger RNA RSUME protein, human transcription factor vasculotropin A VEGFA protein, human article cancer cell culture cell nucleus controlled study correlation analysis down regulation human human cell human tissue hypophysis adenoma hypoxia protein expression protein function protein induction protein secretion protein transport RNA interference upregulation adenoma animal anoxia biosynthesis genetics hypophysis tumor metabolism mouse neovascularization (pathology) tumor cell culture tumor cell line Adenoma Animals Anoxia Cell Line, Tumor Cobalt Humans Hypoxia-Inducible Factor 1, alpha Subunit Mice Neovascularization, Pathologic Pituitary Neoplasms RNA, Messenger Transcription Factors Tumor Cells, Cultured Vascular Endothelial Growth Factor A
spellingShingle	cobalt chloride hypoxia inducible factor 1alpha messenger RNA protein protein RSUME small interfering RNA unclassified drug vasculotropin A cobalt HIF1A protein, human hypoxia inducible factor 1alpha messenger RNA RSUME protein, human transcription factor vasculotropin A VEGFA protein, human article cancer cell culture cell nucleus controlled study correlation analysis down regulation human human cell human tissue hypophysis adenoma hypoxia protein expression protein function protein induction protein secretion protein transport RNA interference upregulation adenoma animal anoxia biosynthesis genetics hypophysis tumor metabolism mouse neovascularization (pathology) tumor cell culture tumor cell line Adenoma Animals Anoxia Cell Line, Tumor Cobalt Humans Hypoxia-Inducible Factor 1, alpha Subunit Mice Neovascularization, Pathologic Pituitary Neoplasms RNA, Messenger Transcription Factors Tumor Cells, Cultured Vascular Endothelial Growth Factor A Shan, B. Gerez, J. Haedo, M. Fuertes, M. Theodoropoulou, M. Buchfelder, M. Losa, M. Stalla, G.K. Arzt, E. Renner, U. RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
topic_facet	cobalt chloride hypoxia inducible factor 1alpha messenger RNA protein protein RSUME small interfering RNA unclassified drug vasculotropin A cobalt HIF1A protein, human hypoxia inducible factor 1alpha messenger RNA RSUME protein, human transcription factor vasculotropin A VEGFA protein, human article cancer cell culture cell nucleus controlled study correlation analysis down regulation human human cell human tissue hypophysis adenoma hypoxia protein expression protein function protein induction protein secretion protein transport RNA interference upregulation adenoma animal anoxia biosynthesis genetics hypophysis tumor metabolism mouse neovascularization (pathology) tumor cell culture tumor cell line Adenoma Animals Anoxia Cell Line, Tumor Cobalt Humans Hypoxia-Inducible Factor 1, alpha Subunit Mice Neovascularization, Pathologic Pituitary Neoplasms RNA, Messenger Transcription Factors Tumor Cells, Cultured Vascular Endothelial Growth Factor A
description	The recently cloned small RWD-domain containing protein RSUME was shown to increase protein levels of hypoxia-inducible factor-1α (HIF-1α). The latter is the oxygen-regulated subunit of HIF-1, the most important transcription factor of the cellular adaptive processes to hypoxic conditions. It is also a major regulator of vascular endothelial growth factor-A (VEGF-A), which is critically involved in the complex process of tumour neovascularisation. In this study, the expression and role of RSUME in pituitary tumours was studied. We found that RSUME mRNA was up-regulated in pituitary adenomas and significantly correlated with HIF-1α mRNA levels. Hypoxia (1% O 2) or treatment with hypoxia-mimicking CoCl 2 enhanced RSUME and HIF-1α expression, induced translocation of HIF-1α to the nuclei and stimulated VEGF-A production both in pituitary tumour cell lines and primary human pituitary adenoma cell cultures. When RSUME expression was specifically down-regulated by siRNA, the CoCl 2-induced increase VEGF-A secretion was strongly reduced which was shown to be a consequence of the RSUME knockdown-associated reduction of HIF-1α synthesis. Thus, RSUME plays an important role in initiating pituitary tumour neovascularisation through regulating HIF-1α levels and subsequent VEGF-A production and may therefore be critically involved in pituitary adenoma progression. © 2012 Society for Endocrinology Printed in Great Britain.
format	JOUR
author	Shan, B. Gerez, J. Haedo, M. Fuertes, M. Theodoropoulou, M. Buchfelder, M. Losa, M. Stalla, G.K. Arzt, E. Renner, U.
author_facet	Shan, B. Gerez, J. Haedo, M. Fuertes, M. Theodoropoulou, M. Buchfelder, M. Losa, M. Stalla, G.K. Arzt, E. Renner, U.
author_sort	Shan, B.
title	RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
title_short	RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
title_full	RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
title_fullStr	RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
title_full_unstemmed	RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells
title_sort	rsume is implicated in hif-1-induced vegf-a production in pituitary tumour cells
url	http://hdl.handle.net/20.500.12110/paper_13510088_v19_n1_p13_Shan
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_version_	1782023936550109184

RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells

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