Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis

Most cells are naturally resistant to TNF-α-induced cell death and become sensitized when NF-κB transactivation is blocked or in the presence of protein synthesis inhibitors that prevent the expression of anti-apoptotic genes. In this report we analyzed the role of osmotic stress on TNIF-α-induced c...

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Autores principales:	Franco, D.L., Nojek, I.M., Molinero, L., Coso, O.A., Costas, M.A.
Formato:	JOUR
Materias:	Apoptosis MAP kinases NF-κB TNF-α immunoglobulin enhancer binding protein phosphotransferase protein bcl 2 protein synthesis inhibitor synaptophysin tumor necrosis factor alpha apoptosis article cell death controlled study enzyme activity enzyme inhibition gene activity gene expression HeLa cell human human cell osmotic stress priority journal protein synthesis inhibition sensitization transactivation Dose-Response Relationship, Drug Enzyme Inhibitors Eukaryotic Cells Hela Cells Humans MAP Kinase Signaling System Mitogen-Activated Protein Kinase 12 Mitogen-Activated Protein Kinase 8 Mitogen-Activated Protein Kinases NF-kappa B Osmotic Pressure p38 Mitogen-Activated Protein Kinases Proto-Oncogene Proteins c-bcl-2 Saline Solution, Hypertonic Stress Sulfasalazine Transcription Factor RelA Tumor Necrosis Factor-alpha
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_13509047_v9_n10_p1090_Franco
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_13509047_v9_n10_p1090_Franco
record_format	dspace
spelling	todo:paper_13509047_v9_n10_p1090_Franco2023-10-03T16:10:04Z Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis Franco, D.L. Nojek, I.M. Molinero, L. Coso, O.A. Costas, M.A. Apoptosis MAP kinases NF-κB TNF-α immunoglobulin enhancer binding protein phosphotransferase protein bcl 2 protein synthesis inhibitor synaptophysin tumor necrosis factor alpha apoptosis article cell death controlled study enzyme activity enzyme inhibition gene activity gene expression HeLa cell human human cell osmotic stress priority journal protein synthesis inhibition sensitization transactivation Apoptosis Dose-Response Relationship, Drug Enzyme Inhibitors Eukaryotic Cells Hela Cells Humans MAP Kinase Signaling System Mitogen-Activated Protein Kinase 12 Mitogen-Activated Protein Kinase 8 Mitogen-Activated Protein Kinases NF-kappa B Osmotic Pressure p38 Mitogen-Activated Protein Kinases Proto-Oncogene Proteins c-bcl-2 Saline Solution, Hypertonic Stress Sulfasalazine Transcription Factor RelA Tumor Necrosis Factor-alpha Most cells are naturally resistant to TNF-α-induced cell death and become sensitized when NF-κB transactivation is blocked or in the presence of protein synthesis inhibitors that prevent the expression of anti-apoptotic genes. In this report we analyzed the role of osmotic stress on TNIF-α-induced cell death. We found that it sensitizes the naturally resistant HeLa cells to TNF-α-induced apoptosis, with the involvement of an increase in the activity of several kinases, the inhibition of Bcl-2 expression, and a late increase on NF-κB activation. Cell death occurs regardless of the enhanced NF-κB activity, whose inhibition produces an increase in apoptosis. The inhibition of p38 kinase, also involved in NF-κB activation, significantly increases the effect of osmotic stress on TNF-α-induced cell death. Fil:Franco, D.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nojek, I.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Molinero, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coso, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Costas, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_13509047_v9_n10_p1090_Franco
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Apoptosis MAP kinases NF-κB TNF-α immunoglobulin enhancer binding protein phosphotransferase protein bcl 2 protein synthesis inhibitor synaptophysin tumor necrosis factor alpha apoptosis article cell death controlled study enzyme activity enzyme inhibition gene activity gene expression HeLa cell human human cell osmotic stress priority journal protein synthesis inhibition sensitization transactivation Apoptosis Dose-Response Relationship, Drug Enzyme Inhibitors Eukaryotic Cells Hela Cells Humans MAP Kinase Signaling System Mitogen-Activated Protein Kinase 12 Mitogen-Activated Protein Kinase 8 Mitogen-Activated Protein Kinases NF-kappa B Osmotic Pressure p38 Mitogen-Activated Protein Kinases Proto-Oncogene Proteins c-bcl-2 Saline Solution, Hypertonic Stress Sulfasalazine Transcription Factor RelA Tumor Necrosis Factor-alpha
spellingShingle	Apoptosis MAP kinases NF-κB TNF-α immunoglobulin enhancer binding protein phosphotransferase protein bcl 2 protein synthesis inhibitor synaptophysin tumor necrosis factor alpha apoptosis article cell death controlled study enzyme activity enzyme inhibition gene activity gene expression HeLa cell human human cell osmotic stress priority journal protein synthesis inhibition sensitization transactivation Apoptosis Dose-Response Relationship, Drug Enzyme Inhibitors Eukaryotic Cells Hela Cells Humans MAP Kinase Signaling System Mitogen-Activated Protein Kinase 12 Mitogen-Activated Protein Kinase 8 Mitogen-Activated Protein Kinases NF-kappa B Osmotic Pressure p38 Mitogen-Activated Protein Kinases Proto-Oncogene Proteins c-bcl-2 Saline Solution, Hypertonic Stress Sulfasalazine Transcription Factor RelA Tumor Necrosis Factor-alpha Franco, D.L. Nojek, I.M. Molinero, L. Coso, O.A. Costas, M.A. Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis
topic_facet	Apoptosis MAP kinases NF-κB TNF-α immunoglobulin enhancer binding protein phosphotransferase protein bcl 2 protein synthesis inhibitor synaptophysin tumor necrosis factor alpha apoptosis article cell death controlled study enzyme activity enzyme inhibition gene activity gene expression HeLa cell human human cell osmotic stress priority journal protein synthesis inhibition sensitization transactivation Apoptosis Dose-Response Relationship, Drug Enzyme Inhibitors Eukaryotic Cells Hela Cells Humans MAP Kinase Signaling System Mitogen-Activated Protein Kinase 12 Mitogen-Activated Protein Kinase 8 Mitogen-Activated Protein Kinases NF-kappa B Osmotic Pressure p38 Mitogen-Activated Protein Kinases Proto-Oncogene Proteins c-bcl-2 Saline Solution, Hypertonic Stress Sulfasalazine Transcription Factor RelA Tumor Necrosis Factor-alpha
description	Most cells are naturally resistant to TNF-α-induced cell death and become sensitized when NF-κB transactivation is blocked or in the presence of protein synthesis inhibitors that prevent the expression of anti-apoptotic genes. In this report we analyzed the role of osmotic stress on TNIF-α-induced cell death. We found that it sensitizes the naturally resistant HeLa cells to TNF-α-induced apoptosis, with the involvement of an increase in the activity of several kinases, the inhibition of Bcl-2 expression, and a late increase on NF-κB activation. Cell death occurs regardless of the enhanced NF-κB activity, whose inhibition produces an increase in apoptosis. The inhibition of p38 kinase, also involved in NF-κB activation, significantly increases the effect of osmotic stress on TNF-α-induced cell death.
format	JOUR
author	Franco, D.L. Nojek, I.M. Molinero, L. Coso, O.A. Costas, M.A.
author_facet	Franco, D.L. Nojek, I.M. Molinero, L. Coso, O.A. Costas, M.A.
author_sort	Franco, D.L.
title	Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis
title_short	Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis
title_full	Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis
title_fullStr	Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis
title_full_unstemmed	Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis
title_sort	osmotic stress sensitizes naturally resistant cells to tnf-α-induced apoptosis
url	http://hdl.handle.net/20.500.12110/paper_13509047_v9_n10_p1090_Franco
work_keys_str_mv	AT francodl osmoticstresssensitizesnaturallyresistantcellstotnfainducedapoptosis AT nojekim osmoticstresssensitizesnaturallyresistantcellstotnfainducedapoptosis AT molinerol osmoticstresssensitizesnaturallyresistantcellstotnfainducedapoptosis AT cosooa osmoticstresssensitizesnaturallyresistantcellstotnfainducedapoptosis AT costasma osmoticstresssensitizesnaturallyresistantcellstotnfainducedapoptosis
_version_	1807318063311224832

Osmotic stress sensitizes naturally resistant cells to TNF-α-induced apoptosis

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