Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium

Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). Enzyme activity was measured throughout the different growth phases of B. megaterium and was found t...

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Detalles Bibliográficos
Autores principales: Vazquez, G.J., Pettinari, M.J., Méndez, B.S.
Formato: JOUR
Materias:
Bacillus megaterium
Polyhydroxybutyrate Phosphotransbutyrylase
enzyme
glucose
isoleucine
phosphotransbutyrylase
poly(3 hydroxybutyric acid)
unclassified drug
valine
article
bacterial growth
controlled study
correlation analysis
enzyme activity
gene location
genetic code
growth curve
nonhuman
protein expression
Bacteria (microorganisms)
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_11396709_v6_n2_p127_Vazquez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). Enzyme activity was measured throughout the different growth phases of B. megaterium and was found to correlate with PHB accumulation during the late-exponential growth phase. Ptb expression was repressed by glucose and activated by the branched amino acids isoleucine and valine. Overexpression of ActBm, a σ 54 regulator from B. megaterium whose gene is located upstream from ptb, caused an increase in Ptb activity and PHB accumulation in B. megaterium.

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