Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium

Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). Enzyme activity was measured throughout the different growth phases of B. megaterium and was found t...

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Autores principales: Vazquez, G.J., Pettinari, M.J., Méndez, B.S.
Formato: JOUR
Materias:
Bacillus megaterium
Polyhydroxybutyrate Phosphotransbutyrylase
enzyme
glucose
isoleucine
phosphotransbutyrylase
poly(3 hydroxybutyric acid)
unclassified drug
valine
article
bacterial growth
controlled study
correlation analysis
enzyme activity
gene location
genetic code
growth curve
nonhuman
protein expression
Bacteria (microorganisms)
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_11396709_v6_n2_p127_Vazquez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_11396709_v6_n2_p127_Vazquez
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spelling todo:paper_11396709_v6_n2_p127_Vazquez2023-10-03T16:08:02Z Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium Vazquez, G.J. Pettinari, M.J. Méndez, B.S. Bacillus megaterium Polyhydroxybutyrate Phosphotransbutyrylase enzyme glucose isoleucine phosphotransbutyrylase poly(3 hydroxybutyric acid) unclassified drug valine article Bacillus megaterium bacterial growth controlled study correlation analysis enzyme activity gene location genetic code growth curve nonhuman protein expression Bacillus megaterium Bacteria (microorganisms) Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). Enzyme activity was measured throughout the different growth phases of B. megaterium and was found to correlate with PHB accumulation during the late-exponential growth phase. Ptb expression was repressed by glucose and activated by the branched amino acids isoleucine and valine. Overexpression of ActBm, a σ 54 regulator from B. megaterium whose gene is located upstream from ptb, caused an increase in Ptb activity and PHB accumulation in B. megaterium. Fil:Vazquez, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_11396709_v6_n2_p127_Vazquez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bacillus megaterium
Polyhydroxybutyrate Phosphotransbutyrylase
enzyme
glucose
isoleucine
phosphotransbutyrylase
poly(3 hydroxybutyric acid)
unclassified drug
valine
article
Bacillus megaterium
bacterial growth
controlled study
correlation analysis
enzyme activity
gene location
genetic code
growth curve
nonhuman
protein expression
Bacillus megaterium
Bacteria (microorganisms)
spellingShingle Bacillus megaterium
Polyhydroxybutyrate Phosphotransbutyrylase
enzyme
glucose
isoleucine
phosphotransbutyrylase
poly(3 hydroxybutyric acid)
unclassified drug
valine
article
Bacillus megaterium
bacterial growth
controlled study
correlation analysis
enzyme activity
gene location
genetic code
growth curve
nonhuman
protein expression
Bacillus megaterium
Bacteria (microorganisms)
Vazquez, G.J.
Pettinari, M.J.
Méndez, B.S.
Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium
topic_facet Bacillus megaterium
Polyhydroxybutyrate Phosphotransbutyrylase
enzyme
glucose
isoleucine
phosphotransbutyrylase
poly(3 hydroxybutyric acid)
unclassified drug
valine
article
Bacillus megaterium
bacterial growth
controlled study
correlation analysis
enzyme activity
gene location
genetic code
growth curve
nonhuman
protein expression
Bacillus megaterium
Bacteria (microorganisms)
description Molecular analysis of a genomic region of Bacillus megaterium, a polyhydroxybutyrate (PHB)-producing microorganism, revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). Enzyme activity was measured throughout the different growth phases of B. megaterium and was found to correlate with PHB accumulation during the late-exponential growth phase. Ptb expression was repressed by glucose and activated by the branched amino acids isoleucine and valine. Overexpression of ActBm, a σ 54 regulator from B. megaterium whose gene is located upstream from ptb, caused an increase in Ptb activity and PHB accumulation in B. megaterium.
format JOUR
author Vazquez, G.J.
Pettinari, M.J.
Méndez, B.S.
author_facet Vazquez, G.J.
Pettinari, M.J.
Méndez, B.S.
author_sort Vazquez, G.J.
title Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium
title_short Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium
title_full Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium
title_fullStr Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium
title_full_unstemmed Evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in Bacillus megaterium
title_sort evidence of an association between poly(3-hydroxybutyrate) accumulation and phosphotransbutyrylase expression in bacillus megaterium
url http://hdl.handle.net/20.500.12110/paper_11396709_v6_n2_p127_Vazquez
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AT pettinarimj evidenceofanassociationbetweenpoly3hydroxybutyrateaccumulationandphosphotransbutyrylaseexpressioninbacillusmegaterium
AT mendezbs evidenceofanassociationbetweenpoly3hydroxybutyrateaccumulationandphosphotransbutyrylaseexpressioninbacillusmegaterium
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