Probable gamma-aminobutyric acid involvement in bisphenol A effect at the hypothalamic level in adult male rats

The aim of the present study was to investigate the effects of bisphenol A (BPA) on the neuroendocrine mechanism of control of the reproductive axis in adult male rats exposed to it during pre- and early postnatal periods. Wistar mated rats were treated with either 0.1% ethanol or BPA in their drink...

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Autores principales:	Cardoso, N., Pandolfi, M., Lavalle, J., Carbone, S., Ponzo, O., Scacchi, P., Reynoso, R.
Formato:	INPR
Lenguaje:	English
Materias:	Bisphenol A GABA Gn-RH Gonadotropins Testosterone
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_11387548_v_n_p1_Cardoso
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_11387548_v_n_p1_Cardoso
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spelling	todo:paper_11387548_v_n_p1_Cardoso2023-10-03T16:08:00Z Probable gamma-aminobutyric acid involvement in bisphenol A effect at the hypothalamic level in adult male rats Cardoso, N. Pandolfi, M. Lavalle, J. Carbone, S. Ponzo, O. Scacchi, P. Reynoso, R. Bisphenol A GABA Gn-RH Gonadotropins Testosterone The aim of the present study was to investigate the effects of bisphenol A (BPA) on the neuroendocrine mechanism of control of the reproductive axis in adult male rats exposed to it during pre- and early postnatal periods. Wistar mated rats were treated with either 0.1% ethanol or BPA in their drinking water until their offspring were weaned at the age of 21 days. The estimated average dose of exposure to dams was approximately 2.5 mg/kg body weight per day of BPA. After 21 days, the pups were separated from the mother and sacrificed on 70 day of life. Gn-RH and gamma-aminobutyric acid (GABA) release from hypothalamic fragments was measured. LH, FSH, and testosterone concentrations were determined, and histological and morphometrical studies of testis were performed. Gn-RH release decreased significantly, while GABA serum levels were markedly increased by treatment. LH serum levels showed no changes, and FSH and testosterone levels decreased significantly. Histological studies showed abnormalities in the tubular organization of the germinal epithelium. The cytoarchitecture of germinal cells was apparently normal, and a reduction of the nuclear area of Leydig cells but not their number was observed. Taken all together, these results provide evidence of the effect caused by BPA on the adult male reproductive axis when exposed during pre- and postnatal period. Moreover, our findings suggest a probable GABA involvement in its effect at the hypothalamic level. © 2011 University of Navarra. Fil:Pandolfi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. INPR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_11387548_v_n_p1_Cardoso
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language	English
orig_language_str_mv	English
topic	Bisphenol A GABA Gn-RH Gonadotropins Testosterone
spellingShingle	Bisphenol A GABA Gn-RH Gonadotropins Testosterone Cardoso, N. Pandolfi, M. Lavalle, J. Carbone, S. Ponzo, O. Scacchi, P. Reynoso, R. Probable gamma-aminobutyric acid involvement in bisphenol A effect at the hypothalamic level in adult male rats
topic_facet	Bisphenol A GABA Gn-RH Gonadotropins Testosterone
description	The aim of the present study was to investigate the effects of bisphenol A (BPA) on the neuroendocrine mechanism of control of the reproductive axis in adult male rats exposed to it during pre- and early postnatal periods. Wistar mated rats were treated with either 0.1% ethanol or BPA in their drinking water until their offspring were weaned at the age of 21 days. The estimated average dose of exposure to dams was approximately 2.5 mg/kg body weight per day of BPA. After 21 days, the pups were separated from the mother and sacrificed on 70 day of life. Gn-RH and gamma-aminobutyric acid (GABA) release from hypothalamic fragments was measured. LH, FSH, and testosterone concentrations were determined, and histological and morphometrical studies of testis were performed. Gn-RH release decreased significantly, while GABA serum levels were markedly increased by treatment. LH serum levels showed no changes, and FSH and testosterone levels decreased significantly. Histological studies showed abnormalities in the tubular organization of the germinal epithelium. The cytoarchitecture of germinal cells was apparently normal, and a reduction of the nuclear area of Leydig cells but not their number was observed. Taken all together, these results provide evidence of the effect caused by BPA on the adult male reproductive axis when exposed during pre- and postnatal period. Moreover, our findings suggest a probable GABA involvement in its effect at the hypothalamic level. © 2011 University of Navarra.
format	INPR
author	Cardoso, N. Pandolfi, M. Lavalle, J. Carbone, S. Ponzo, O. Scacchi, P. Reynoso, R.
author_facet	Cardoso, N. Pandolfi, M. Lavalle, J. Carbone, S. Ponzo, O. Scacchi, P. Reynoso, R.
author_sort	Cardoso, N.
title	Probable gamma-aminobutyric acid involvement in bisphenol A effect at the hypothalamic level in adult male rats
title_short	Probable gamma-aminobutyric acid involvement in bisphenol A effect at the hypothalamic level in adult male rats
title_full	Probable gamma-aminobutyric acid involvement in bisphenol A effect at the hypothalamic level in adult male rats
title_fullStr	Probable gamma-aminobutyric acid involvement in bisphenol A effect at the hypothalamic level in adult male rats
title_full_unstemmed	Probable gamma-aminobutyric acid involvement in bisphenol A effect at the hypothalamic level in adult male rats
title_sort	probable gamma-aminobutyric acid involvement in bisphenol a effect at the hypothalamic level in adult male rats
url	http://hdl.handle.net/20.500.12110/paper_11387548_v_n_p1_Cardoso
work_keys_str_mv	AT cardoson probablegammaaminobutyricacidinvolvementinbisphenolaeffectatthehypothalamiclevelinadultmalerats AT pandolfim probablegammaaminobutyricacidinvolvementinbisphenolaeffectatthehypothalamiclevelinadultmalerats AT lavallej probablegammaaminobutyricacidinvolvementinbisphenolaeffectatthehypothalamiclevelinadultmalerats AT carbones probablegammaaminobutyricacidinvolvementinbisphenolaeffectatthehypothalamiclevelinadultmalerats AT ponzoo probablegammaaminobutyricacidinvolvementinbisphenolaeffectatthehypothalamiclevelinadultmalerats AT scacchip probablegammaaminobutyricacidinvolvementinbisphenolaeffectatthehypothalamiclevelinadultmalerats AT reynosor probablegammaaminobutyricacidinvolvementinbisphenolaeffectatthehypothalamiclevelinadultmalerats
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Probable gamma-aminobutyric acid involvement in bisphenol A effect at the hypothalamic level in adult male rats

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