Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat
11 beta-hydroxysteroid dehydrogenase (HSDs) enzymes regulate the activity of glucocorticoids in target organs. HSD1, one of the two existing isoforms, locates mainly in CNS, liver and adipose tissue. HSD1 is involved in the pathogenesis of diseases such as obesity, insulin resistance, arterial hyper...
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todo:paper_11387548_v65_n4_p329_Altuna2023-10-03T16:07:59Z Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat Altuna, M.E. Mazzetti, M.B. Rago, L.F. De Viale, L.C.S.M. Damasco, M.C. Glucose Glycyrrhetinic acid HSD1 Pepck Stress 11beta hydroxysteroid dehydrogenase 1 carbohydrate chloride glucose glycogen phosphorylase glycyrrhetinic acid phosphoenolpyruvate carboxykinase (ATP) acidity animal experiment animal tissue article carbohydrate metabolism controlled study enzyme activity glucose blood level glucose metabolism male metabolic syndrome X non insulin dependent diabetes mellitus nonhuman rat 11-beta-Hydroxysteroid Dehydrogenases Adipose Tissue Animals Carbohydrate Metabolism Carbohydrates Central Nervous System Glucose Glycyrrhetinic Acid Liver Male Models, Biological Phosphoenolpyruvate Carboxykinase (ATP) Protein Isoforms Rats Rats, Sprague-Dawley Animalia Rattus 11 beta-hydroxysteroid dehydrogenase (HSDs) enzymes regulate the activity of glucocorticoids in target organs. HSD1, one of the two existing isoforms, locates mainly in CNS, liver and adipose tissue. HSD1 is involved in the pathogenesis of diseases such as obesity, insulin resistance, arterial hypertension and the Metabolic Syndrome. The stress produced by HC1 overload triggers metabolic acidosis and increases liver HSD1 activity associated with increased phosphoenolpyruvate carboxykinase, a regulatory enzyme of gluconeogenesis that is activated by glucocorticoids, with increased glycaemia and glycogen breakdown. The aim of this study was to analyze whether the metabolic modifications triggered by HC1 stress are due to increased liver HSD1 activity. Glycyrrhetinic acid, a potent HDS inhibitor, was administered subcutaneously (20 mg/ml) to stressed and unstressed four months old male Sprague Dawley rats to investigate changes in liver HSD1, phosphoenolpyruvate carboxykinase (PECPK) and glycogen phosphorylase activities and plasma glucose levels. It was observed that all these parameters increased in stressed animals, but that treatment with glycyrrhetinic acid significantly reduced their levels. In conclusion, our results demonstrate the involvement of HSD1 in stress induced carbohydrate disturbances and could contribute to the impact of HSD1 inhibitors on carbohydrate metabolism and its relevance in the study of Metabolic Syndrome Disorder and non insulin-dependent diabetes mellitus. Fil:Mazzetti, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_11387548_v65_n4_p329_Altuna |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Glucose Glycyrrhetinic acid HSD1 Pepck Stress 11beta hydroxysteroid dehydrogenase 1 carbohydrate chloride glucose glycogen phosphorylase glycyrrhetinic acid phosphoenolpyruvate carboxykinase (ATP) acidity animal experiment animal tissue article carbohydrate metabolism controlled study enzyme activity glucose blood level glucose metabolism male metabolic syndrome X non insulin dependent diabetes mellitus nonhuman rat 11-beta-Hydroxysteroid Dehydrogenases Adipose Tissue Animals Carbohydrate Metabolism Carbohydrates Central Nervous System Glucose Glycyrrhetinic Acid Liver Male Models, Biological Phosphoenolpyruvate Carboxykinase (ATP) Protein Isoforms Rats Rats, Sprague-Dawley Animalia Rattus |
spellingShingle |
Glucose Glycyrrhetinic acid HSD1 Pepck Stress 11beta hydroxysteroid dehydrogenase 1 carbohydrate chloride glucose glycogen phosphorylase glycyrrhetinic acid phosphoenolpyruvate carboxykinase (ATP) acidity animal experiment animal tissue article carbohydrate metabolism controlled study enzyme activity glucose blood level glucose metabolism male metabolic syndrome X non insulin dependent diabetes mellitus nonhuman rat 11-beta-Hydroxysteroid Dehydrogenases Adipose Tissue Animals Carbohydrate Metabolism Carbohydrates Central Nervous System Glucose Glycyrrhetinic Acid Liver Male Models, Biological Phosphoenolpyruvate Carboxykinase (ATP) Protein Isoforms Rats Rats, Sprague-Dawley Animalia Rattus Altuna, M.E. Mazzetti, M.B. Rago, L.F. De Viale, L.C.S.M. Damasco, M.C. Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat |
topic_facet |
Glucose Glycyrrhetinic acid HSD1 Pepck Stress 11beta hydroxysteroid dehydrogenase 1 carbohydrate chloride glucose glycogen phosphorylase glycyrrhetinic acid phosphoenolpyruvate carboxykinase (ATP) acidity animal experiment animal tissue article carbohydrate metabolism controlled study enzyme activity glucose blood level glucose metabolism male metabolic syndrome X non insulin dependent diabetes mellitus nonhuman rat 11-beta-Hydroxysteroid Dehydrogenases Adipose Tissue Animals Carbohydrate Metabolism Carbohydrates Central Nervous System Glucose Glycyrrhetinic Acid Liver Male Models, Biological Phosphoenolpyruvate Carboxykinase (ATP) Protein Isoforms Rats Rats, Sprague-Dawley Animalia Rattus |
description |
11 beta-hydroxysteroid dehydrogenase (HSDs) enzymes regulate the activity of glucocorticoids in target organs. HSD1, one of the two existing isoforms, locates mainly in CNS, liver and adipose tissue. HSD1 is involved in the pathogenesis of diseases such as obesity, insulin resistance, arterial hypertension and the Metabolic Syndrome. The stress produced by HC1 overload triggers metabolic acidosis and increases liver HSD1 activity associated with increased phosphoenolpyruvate carboxykinase, a regulatory enzyme of gluconeogenesis that is activated by glucocorticoids, with increased glycaemia and glycogen breakdown. The aim of this study was to analyze whether the metabolic modifications triggered by HC1 stress are due to increased liver HSD1 activity. Glycyrrhetinic acid, a potent HDS inhibitor, was administered subcutaneously (20 mg/ml) to stressed and unstressed four months old male Sprague Dawley rats to investigate changes in liver HSD1, phosphoenolpyruvate carboxykinase (PECPK) and glycogen phosphorylase activities and plasma glucose levels. It was observed that all these parameters increased in stressed animals, but that treatment with glycyrrhetinic acid significantly reduced their levels. In conclusion, our results demonstrate the involvement of HSD1 in stress induced carbohydrate disturbances and could contribute to the impact of HSD1 inhibitors on carbohydrate metabolism and its relevance in the study of Metabolic Syndrome Disorder and non insulin-dependent diabetes mellitus. |
format |
JOUR |
author |
Altuna, M.E. Mazzetti, M.B. Rago, L.F. De Viale, L.C.S.M. Damasco, M.C. |
author_facet |
Altuna, M.E. Mazzetti, M.B. Rago, L.F. De Viale, L.C.S.M. Damasco, M.C. |
author_sort |
Altuna, M.E. |
title |
Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat |
title_short |
Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat |
title_full |
Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat |
title_fullStr |
Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat |
title_full_unstemmed |
Hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat |
title_sort |
hepatic 11 beta-hydroxysteroid dehydrogenase 1 involvement in alterations of glucose metabolism produced by acidotic stress in rat |
url |
http://hdl.handle.net/20.500.12110/paper_11387548_v65_n4_p329_Altuna |
work_keys_str_mv |
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