Stereum hirsutum (Wild) Pers. action in dye degradation
Stereum hirsutum, a white rot fungus, has a good growth in solid state fermentation. This was carried on with wheat bran, soy bran and a mixture of both. Mycelia grown on soy bran showed the highest decolonization activity on Ponceau 2R (xylidine), indigo carmine and malachite green. Optimal relatio...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_11301406_v24_n4_p294_Mouso |
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todo:paper_11301406_v24_n4_p294_Mouso2023-10-03T16:07:51Z Stereum hirsutum (Wild) Pers. action in dye degradation Mouso, N. Diorio, L. Forchiassin, F. Decolorization Detoxification Laccase Stereum hirsutum White rot fungi indigo carmine laccase lignin malachite green article decolorization electrophoresis enzyme synthesis fungus growth microbial degradation mycelial growth nonhuman nutrient Phanerochaete solid state fermentation soybean Stereum hirsutum wheat bran white rot fungus Fungi Phanerochaete chrysosporium Stereum hirsutum Triticum aestivum Stereum hirsutum, a white rot fungus, has a good growth in solid state fermentation. This was carried on with wheat bran, soy bran and a mixture of both. Mycelia grown on soy bran showed the highest decolonization activity on Ponceau 2R (xylidine), indigo carmine and malachite green. Optimal relationship between decolorization and detoxification of malachite green was 30 g of fresh weight (mycelia plus substrate) in 500 ml malachite green solution, 42 U/l of laccase was measured in this solution. Decolorization was carried on without the addition either of nutrients or mediators. Conditions for maximal decolorization did not agree with those for maximal ligninolytic enzyme production, but effectiveness of laccase activity on decolorization was evidenced by electrophoretic analysis, that allowed laccase identification and its decolorization activity in gels stained with indigo carmine and malachite green, with ABTS as mediator. Detoxification was assayed using the sensible fungus Phanerochaete chrysosporium. ©2007 Revista Iberoamericana de Micología. Fil:Mouso, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Diorio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_11301406_v24_n4_p294_Mouso |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Decolorization Detoxification Laccase Stereum hirsutum White rot fungi indigo carmine laccase lignin malachite green article decolorization electrophoresis enzyme synthesis fungus growth microbial degradation mycelial growth nonhuman nutrient Phanerochaete solid state fermentation soybean Stereum hirsutum wheat bran white rot fungus Fungi Phanerochaete chrysosporium Stereum hirsutum Triticum aestivum |
| spellingShingle |
Decolorization Detoxification Laccase Stereum hirsutum White rot fungi indigo carmine laccase lignin malachite green article decolorization electrophoresis enzyme synthesis fungus growth microbial degradation mycelial growth nonhuman nutrient Phanerochaete solid state fermentation soybean Stereum hirsutum wheat bran white rot fungus Fungi Phanerochaete chrysosporium Stereum hirsutum Triticum aestivum Mouso, N. Diorio, L. Forchiassin, F. Stereum hirsutum (Wild) Pers. action in dye degradation |
| topic_facet |
Decolorization Detoxification Laccase Stereum hirsutum White rot fungi indigo carmine laccase lignin malachite green article decolorization electrophoresis enzyme synthesis fungus growth microbial degradation mycelial growth nonhuman nutrient Phanerochaete solid state fermentation soybean Stereum hirsutum wheat bran white rot fungus Fungi Phanerochaete chrysosporium Stereum hirsutum Triticum aestivum |
| description |
Stereum hirsutum, a white rot fungus, has a good growth in solid state fermentation. This was carried on with wheat bran, soy bran and a mixture of both. Mycelia grown on soy bran showed the highest decolonization activity on Ponceau 2R (xylidine), indigo carmine and malachite green. Optimal relationship between decolorization and detoxification of malachite green was 30 g of fresh weight (mycelia plus substrate) in 500 ml malachite green solution, 42 U/l of laccase was measured in this solution. Decolorization was carried on without the addition either of nutrients or mediators. Conditions for maximal decolorization did not agree with those for maximal ligninolytic enzyme production, but effectiveness of laccase activity on decolorization was evidenced by electrophoretic analysis, that allowed laccase identification and its decolorization activity in gels stained with indigo carmine and malachite green, with ABTS as mediator. Detoxification was assayed using the sensible fungus Phanerochaete chrysosporium. ©2007 Revista Iberoamericana de Micología. |
| format |
JOUR |
| author |
Mouso, N. Diorio, L. Forchiassin, F. |
| author_facet |
Mouso, N. Diorio, L. Forchiassin, F. |
| author_sort |
Mouso, N. |
| title |
Stereum hirsutum (Wild) Pers. action in dye degradation |
| title_short |
Stereum hirsutum (Wild) Pers. action in dye degradation |
| title_full |
Stereum hirsutum (Wild) Pers. action in dye degradation |
| title_fullStr |
Stereum hirsutum (Wild) Pers. action in dye degradation |
| title_full_unstemmed |
Stereum hirsutum (Wild) Pers. action in dye degradation |
| title_sort |
stereum hirsutum (wild) pers. action in dye degradation |
| url |
http://hdl.handle.net/20.500.12110/paper_11301406_v24_n4_p294_Mouso |
| work_keys_str_mv |
AT mouson stereumhirsutumwildpersactionindyedegradation AT dioriol stereumhirsutumwildpersactionindyedegradation AT forchiassinf stereumhirsutumwildpersactionindyedegradation |
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1807321176760909824 |