In vitro effects of both dopaminergic and enkephalinergic antagonists on the ovarian growth of Cherax quadricarinatus (Decapoda, Parastacidae), at different periods of the reproductive cycle

The in vitro effect of both spiperone (dopaminergic antagonist) and naloxone (enkephalinergic antagonist), was assayed on small pieces of ovary dissected from C. quadricarinatus females, with the eventual addition of some neuroendocrine organs, such as thoracic ganglion or eyestalk tissue. The incor...

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Autores principales:	Cahansky, A.V., Medesani, D.A., Chaulet, A., Rodríguez, E.M.
Formato:	JOUR
Materias:	Crayfish Dopamine Enkephalin Naloxone Ovary Reproduction Spiperone Thoracic ganglion leucine naloxone spiperone animal cell article Cherax quadricarinatus controlled study crayfish female in vitro study molecular model nonhuman organ growth ovary reproduction stellate ganglion Animals Astacoidea Dopamine Antagonists Female Narcotic Antagonists Species Specificity Crustacea Decapoda (Crustacea) Parastacidae
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10956433_v158_n1_p126_Cahansky
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_10956433_v158_n1_p126_Cahansky
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spelling	todo:paper_10956433_v158_n1_p126_Cahansky2023-10-03T16:05:21Z In vitro effects of both dopaminergic and enkephalinergic antagonists on the ovarian growth of Cherax quadricarinatus (Decapoda, Parastacidae), at different periods of the reproductive cycle Cahansky, A.V. Medesani, D.A. Chaulet, A. Rodríguez, E.M. Crayfish Dopamine Enkephalin Naloxone Ovary Reproduction Spiperone Thoracic ganglion leucine naloxone spiperone animal cell article Cherax quadricarinatus controlled study crayfish female in vitro study molecular model nonhuman organ growth ovary reproduction stellate ganglion Animals Astacoidea Dopamine Antagonists Female Naloxone Narcotic Antagonists Ovary Reproduction Species Specificity Spiperone Astacoidea Cherax quadricarinatus Crustacea Decapoda (Crustacea) Parastacidae The in vitro effect of both spiperone (dopaminergic antagonist) and naloxone (enkephalinergic antagonist), was assayed on small pieces of ovary dissected from C. quadricarinatus females, with the eventual addition of some neuroendocrine organs, such as thoracic ganglion or eyestalk tissue. The incorporation of tritiated leucine by the ovary was measured in order to estimate the ovarian growth. During the post-reproductive period, both mentioned antagonists were able to significantly stimulate the ovary in the presence of thoracic ganglion, but did not produce any significant effect in the preparation containing ovary and eyestalk tissue, or only ovary. No significant effects of the assayed antagonists were noted during the pre-reproductive period. These results were in accordance with previous models describing the neuroendocrine control of crustacean reproduction, and represent new findings about the hormonal context in different periods of the reproductive cycle of crayfish. Besides, by means of the experimental combination of the tested antagonists with dopamine or met-enkephalin, a new model dealing with the interaction of these two neurotransmitters on the hormonal secretion of thoracic ganglion has been proposed. © 2010 Elsevier Inc. Fil:Cahansky, A.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Medesani, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, E.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10956433_v158_n1_p126_Cahansky
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Crayfish Dopamine Enkephalin Naloxone Ovary Reproduction Spiperone Thoracic ganglion leucine naloxone spiperone animal cell article Cherax quadricarinatus controlled study crayfish female in vitro study molecular model nonhuman organ growth ovary reproduction stellate ganglion Animals Astacoidea Dopamine Antagonists Female Naloxone Narcotic Antagonists Ovary Reproduction Species Specificity Spiperone Astacoidea Cherax quadricarinatus Crustacea Decapoda (Crustacea) Parastacidae
spellingShingle	Crayfish Dopamine Enkephalin Naloxone Ovary Reproduction Spiperone Thoracic ganglion leucine naloxone spiperone animal cell article Cherax quadricarinatus controlled study crayfish female in vitro study molecular model nonhuman organ growth ovary reproduction stellate ganglion Animals Astacoidea Dopamine Antagonists Female Naloxone Narcotic Antagonists Ovary Reproduction Species Specificity Spiperone Astacoidea Cherax quadricarinatus Crustacea Decapoda (Crustacea) Parastacidae Cahansky, A.V. Medesani, D.A. Chaulet, A. Rodríguez, E.M. In vitro effects of both dopaminergic and enkephalinergic antagonists on the ovarian growth of Cherax quadricarinatus (Decapoda, Parastacidae), at different periods of the reproductive cycle
topic_facet	Crayfish Dopamine Enkephalin Naloxone Ovary Reproduction Spiperone Thoracic ganglion leucine naloxone spiperone animal cell article Cherax quadricarinatus controlled study crayfish female in vitro study molecular model nonhuman organ growth ovary reproduction stellate ganglion Animals Astacoidea Dopamine Antagonists Female Naloxone Narcotic Antagonists Ovary Reproduction Species Specificity Spiperone Astacoidea Cherax quadricarinatus Crustacea Decapoda (Crustacea) Parastacidae
description	The in vitro effect of both spiperone (dopaminergic antagonist) and naloxone (enkephalinergic antagonist), was assayed on small pieces of ovary dissected from C. quadricarinatus females, with the eventual addition of some neuroendocrine organs, such as thoracic ganglion or eyestalk tissue. The incorporation of tritiated leucine by the ovary was measured in order to estimate the ovarian growth. During the post-reproductive period, both mentioned antagonists were able to significantly stimulate the ovary in the presence of thoracic ganglion, but did not produce any significant effect in the preparation containing ovary and eyestalk tissue, or only ovary. No significant effects of the assayed antagonists were noted during the pre-reproductive period. These results were in accordance with previous models describing the neuroendocrine control of crustacean reproduction, and represent new findings about the hormonal context in different periods of the reproductive cycle of crayfish. Besides, by means of the experimental combination of the tested antagonists with dopamine or met-enkephalin, a new model dealing with the interaction of these two neurotransmitters on the hormonal secretion of thoracic ganglion has been proposed. © 2010 Elsevier Inc.
format	JOUR
author	Cahansky, A.V. Medesani, D.A. Chaulet, A. Rodríguez, E.M.
author_facet	Cahansky, A.V. Medesani, D.A. Chaulet, A. Rodríguez, E.M.
author_sort	Cahansky, A.V.
title	In vitro effects of both dopaminergic and enkephalinergic antagonists on the ovarian growth of Cherax quadricarinatus (Decapoda, Parastacidae), at different periods of the reproductive cycle
title_short	In vitro effects of both dopaminergic and enkephalinergic antagonists on the ovarian growth of Cherax quadricarinatus (Decapoda, Parastacidae), at different periods of the reproductive cycle
title_full	In vitro effects of both dopaminergic and enkephalinergic antagonists on the ovarian growth of Cherax quadricarinatus (Decapoda, Parastacidae), at different periods of the reproductive cycle
title_fullStr	In vitro effects of both dopaminergic and enkephalinergic antagonists on the ovarian growth of Cherax quadricarinatus (Decapoda, Parastacidae), at different periods of the reproductive cycle
title_full_unstemmed	In vitro effects of both dopaminergic and enkephalinergic antagonists on the ovarian growth of Cherax quadricarinatus (Decapoda, Parastacidae), at different periods of the reproductive cycle
title_sort	in vitro effects of both dopaminergic and enkephalinergic antagonists on the ovarian growth of cherax quadricarinatus (decapoda, parastacidae), at different periods of the reproductive cycle
url	http://hdl.handle.net/20.500.12110/paper_10956433_v158_n1_p126_Cahansky
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In vitro effects of both dopaminergic and enkephalinergic antagonists on the ovarian growth of Cherax quadricarinatus (Decapoda, Parastacidae), at different periods of the reproductive cycle

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