In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus

In this work the antiviral activity of 20 dehydroepiandrosterone (DHEA) analogs with different substituents at positions C-3, C-15, C-16 and C-17 were evaluated against vesicular stomatitis virus (VSV) in Vero cell cultures. The selectivity indexes (SI) obtained with DHEA and epiandrosterone (EA) we...

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Autores principales:	Romanutti, C., Bruttomesso, A.C., Castilla, V., Bisceglia, J.A., Galagovsky, L.R., Wachsman, M.B.
Formato:	JOUR
Materias:	Antiviral Dehydroepiandrosterone analogs DHEA EA Vesicular stomatitis virus 1 beta dextro ribofuranosil 1 2 4 triazole 3 carboxamide 16alpha hydroxyprasterone 17 oxo 5alpha androstan 3beta,16alpha yldiacetate 17 oxo 5alpha androstan 3beta,16beta yldiacetate 17 oxoandrost 5 en 3beta yl acetate 17 oxoandrost 5 en 3beta ylacetae 17 oxoandrost 5 en 3beta,16alpha diyldiacetate 17 oxoandrost 5 en 3beta,16beta yldiacetate 17,17 ethylendioxy 3beta androstanyl acetate 17,17 ethylendioxyandrost 5 en 3beta ol 17,17 ethylendioxyandrost 5,15 dien 3beta ol 17,17 ethylendioxyandrostan 16alpha bromo 3beta ol 17,17 ethylendioxyandrostan 3beta ol 17,17 ethylendioxyandrostan 5,15 dien 3beta ol 21 norpregna 5,17(20) dien 3beta,16alpha diyldiacetate 3beta 6alpha dihydroxy 17 androstanone 3beta hydroxypregn 17(20) en 16 one 3beta,15alpha dihydroxy 5alpha androstan 17 one 3beta,15beta dihydroxy 5alpha androstan 17 one 3beta,16alpha dihydroxy 5alpha androstan 17 one androsta 5,16 dien 3beta,17 diyldiacetate antivirus agent epiandrosterone prasterone prasterone derivative ribavirin unclassified drug animal cell antiviral activity article controlled study drug mechanism growth inhibition in vitro study nonhuman protein synthesis RNA synthesis Vero cell virogenesis virus particle virus replication Animals Antiviral Agents Cercopithecus aethiops Dehydroepiandrosterone Fluorescent Antibody Technique, Indirect Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Vero Cells Vesicular Stomatitis Vesiculovirus Virus Replication
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10900233_v182_n2_p327_Romanutti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_10900233_v182_n2_p327_Romanutti2023-10-03T16:04:56Z In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus Romanutti, C. Bruttomesso, A.C. Castilla, V. Bisceglia, J.A. Galagovsky, L.R. Wachsman, M.B. Antiviral Dehydroepiandrosterone analogs DHEA EA Vesicular stomatitis virus 1 beta dextro ribofuranosil 1 2 4 triazole 3 carboxamide 16alpha hydroxyprasterone 17 oxo 5alpha androstan 3beta,16alpha yldiacetate 17 oxo 5alpha androstan 3beta,16beta yldiacetate 17 oxoandrost 5 en 3beta yl acetate 17 oxoandrost 5 en 3beta ylacetae 17 oxoandrost 5 en 3beta,16alpha diyldiacetate 17 oxoandrost 5 en 3beta,16beta yldiacetate 17,17 ethylendioxy 3beta androstanyl acetate 17,17 ethylendioxyandrost 5 en 3beta ol 17,17 ethylendioxyandrost 5,15 dien 3beta ol 17,17 ethylendioxyandrostan 16alpha bromo 3beta ol 17,17 ethylendioxyandrostan 3beta ol 17,17 ethylendioxyandrostan 5,15 dien 3beta ol 21 norpregna 5,17(20) dien 3beta,16alpha diyldiacetate 3beta 6alpha dihydroxy 17 androstanone 3beta hydroxypregn 17(20) en 16 one 3beta,15alpha dihydroxy 5alpha androstan 17 one 3beta,15beta dihydroxy 5alpha androstan 17 one 3beta,16alpha dihydroxy 5alpha androstan 17 one androsta 5,16 dien 3beta,17 diyldiacetate antivirus agent epiandrosterone prasterone prasterone derivative ribavirin unclassified drug animal cell antiviral activity article controlled study drug mechanism growth inhibition in vitro study nonhuman protein synthesis RNA synthesis Vero cell Vesicular stomatitis virus virogenesis virus particle virus replication Animals Antiviral Agents Cercopithecus aethiops Dehydroepiandrosterone Fluorescent Antibody Technique, Indirect Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Vero Cells Vesicular Stomatitis Vesiculovirus Virus Replication Vesicular stomatitis virus In this work the antiviral activity of 20 dehydroepiandrosterone (DHEA) analogs with different substituents at positions C-3, C-15, C-16 and C-17 were evaluated against vesicular stomatitis virus (VSV) in Vero cell cultures. The selectivity indexes (SI) obtained with DHEA and epiandrosterone (EA) were 50 and 72.6, respectively. The work showed that the compounds 21-norpregna-5,17(20)-dien-3β,16α-diyl-diacetate, 17,17-ethylendioxyandrostan-5,15-dien-3β-ol and 3β-hydroxypregn-17(20)-en-16-one had higher SI values than ribavirin, which was used as a reference drug. The antiviral mode of action of DHEA was also investigated against VSV replication in Vero cells, and time of addition experiments showed that DHEA mainly affected a late event in the virus growth cycle. Analysis of RNA and protein synthesis indicated that DHEA adversely affected positive strand RNA synthesis and viral mature particle formation. © 2008 Elsevier Ltd. All rights reserved. Fil:Romanutti, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bruttomesso, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wachsman, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10900233_v182_n2_p327_Romanutti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antiviral Dehydroepiandrosterone analogs DHEA EA Vesicular stomatitis virus 1 beta dextro ribofuranosil 1 2 4 triazole 3 carboxamide 16alpha hydroxyprasterone 17 oxo 5alpha androstan 3beta,16alpha yldiacetate 17 oxo 5alpha androstan 3beta,16beta yldiacetate 17 oxoandrost 5 en 3beta yl acetate 17 oxoandrost 5 en 3beta ylacetae 17 oxoandrost 5 en 3beta,16alpha diyldiacetate 17 oxoandrost 5 en 3beta,16beta yldiacetate 17,17 ethylendioxy 3beta androstanyl acetate 17,17 ethylendioxyandrost 5 en 3beta ol 17,17 ethylendioxyandrost 5,15 dien 3beta ol 17,17 ethylendioxyandrostan 16alpha bromo 3beta ol 17,17 ethylendioxyandrostan 3beta ol 17,17 ethylendioxyandrostan 5,15 dien 3beta ol 21 norpregna 5,17(20) dien 3beta,16alpha diyldiacetate 3beta 6alpha dihydroxy 17 androstanone 3beta hydroxypregn 17(20) en 16 one 3beta,15alpha dihydroxy 5alpha androstan 17 one 3beta,15beta dihydroxy 5alpha androstan 17 one 3beta,16alpha dihydroxy 5alpha androstan 17 one androsta 5,16 dien 3beta,17 diyldiacetate antivirus agent epiandrosterone prasterone prasterone derivative ribavirin unclassified drug animal cell antiviral activity article controlled study drug mechanism growth inhibition in vitro study nonhuman protein synthesis RNA synthesis Vero cell Vesicular stomatitis virus virogenesis virus particle virus replication Animals Antiviral Agents Cercopithecus aethiops Dehydroepiandrosterone Fluorescent Antibody Technique, Indirect Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Vero Cells Vesicular Stomatitis Vesiculovirus Virus Replication Vesicular stomatitis virus
spellingShingle	Antiviral Dehydroepiandrosterone analogs DHEA EA Vesicular stomatitis virus 1 beta dextro ribofuranosil 1 2 4 triazole 3 carboxamide 16alpha hydroxyprasterone 17 oxo 5alpha androstan 3beta,16alpha yldiacetate 17 oxo 5alpha androstan 3beta,16beta yldiacetate 17 oxoandrost 5 en 3beta yl acetate 17 oxoandrost 5 en 3beta ylacetae 17 oxoandrost 5 en 3beta,16alpha diyldiacetate 17 oxoandrost 5 en 3beta,16beta yldiacetate 17,17 ethylendioxy 3beta androstanyl acetate 17,17 ethylendioxyandrost 5 en 3beta ol 17,17 ethylendioxyandrost 5,15 dien 3beta ol 17,17 ethylendioxyandrostan 16alpha bromo 3beta ol 17,17 ethylendioxyandrostan 3beta ol 17,17 ethylendioxyandrostan 5,15 dien 3beta ol 21 norpregna 5,17(20) dien 3beta,16alpha diyldiacetate 3beta 6alpha dihydroxy 17 androstanone 3beta hydroxypregn 17(20) en 16 one 3beta,15alpha dihydroxy 5alpha androstan 17 one 3beta,15beta dihydroxy 5alpha androstan 17 one 3beta,16alpha dihydroxy 5alpha androstan 17 one androsta 5,16 dien 3beta,17 diyldiacetate antivirus agent epiandrosterone prasterone prasterone derivative ribavirin unclassified drug animal cell antiviral activity article controlled study drug mechanism growth inhibition in vitro study nonhuman protein synthesis RNA synthesis Vero cell Vesicular stomatitis virus virogenesis virus particle virus replication Animals Antiviral Agents Cercopithecus aethiops Dehydroepiandrosterone Fluorescent Antibody Technique, Indirect Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Vero Cells Vesicular Stomatitis Vesiculovirus Virus Replication Vesicular stomatitis virus Romanutti, C. Bruttomesso, A.C. Castilla, V. Bisceglia, J.A. Galagovsky, L.R. Wachsman, M.B. In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus
topic_facet	Antiviral Dehydroepiandrosterone analogs DHEA EA Vesicular stomatitis virus 1 beta dextro ribofuranosil 1 2 4 triazole 3 carboxamide 16alpha hydroxyprasterone 17 oxo 5alpha androstan 3beta,16alpha yldiacetate 17 oxo 5alpha androstan 3beta,16beta yldiacetate 17 oxoandrost 5 en 3beta yl acetate 17 oxoandrost 5 en 3beta ylacetae 17 oxoandrost 5 en 3beta,16alpha diyldiacetate 17 oxoandrost 5 en 3beta,16beta yldiacetate 17,17 ethylendioxy 3beta androstanyl acetate 17,17 ethylendioxyandrost 5 en 3beta ol 17,17 ethylendioxyandrost 5,15 dien 3beta ol 17,17 ethylendioxyandrostan 16alpha bromo 3beta ol 17,17 ethylendioxyandrostan 3beta ol 17,17 ethylendioxyandrostan 5,15 dien 3beta ol 21 norpregna 5,17(20) dien 3beta,16alpha diyldiacetate 3beta 6alpha dihydroxy 17 androstanone 3beta hydroxypregn 17(20) en 16 one 3beta,15alpha dihydroxy 5alpha androstan 17 one 3beta,15beta dihydroxy 5alpha androstan 17 one 3beta,16alpha dihydroxy 5alpha androstan 17 one androsta 5,16 dien 3beta,17 diyldiacetate antivirus agent epiandrosterone prasterone prasterone derivative ribavirin unclassified drug animal cell antiviral activity article controlled study drug mechanism growth inhibition in vitro study nonhuman protein synthesis RNA synthesis Vero cell Vesicular stomatitis virus virogenesis virus particle virus replication Animals Antiviral Agents Cercopithecus aethiops Dehydroepiandrosterone Fluorescent Antibody Technique, Indirect Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Vero Cells Vesicular Stomatitis Vesiculovirus Virus Replication Vesicular stomatitis virus
description	In this work the antiviral activity of 20 dehydroepiandrosterone (DHEA) analogs with different substituents at positions C-3, C-15, C-16 and C-17 were evaluated against vesicular stomatitis virus (VSV) in Vero cell cultures. The selectivity indexes (SI) obtained with DHEA and epiandrosterone (EA) were 50 and 72.6, respectively. The work showed that the compounds 21-norpregna-5,17(20)-dien-3β,16α-diyl-diacetate, 17,17-ethylendioxyandrostan-5,15-dien-3β-ol and 3β-hydroxypregn-17(20)-en-16-one had higher SI values than ribavirin, which was used as a reference drug. The antiviral mode of action of DHEA was also investigated against VSV replication in Vero cells, and time of addition experiments showed that DHEA mainly affected a late event in the virus growth cycle. Analysis of RNA and protein synthesis indicated that DHEA adversely affected positive strand RNA synthesis and viral mature particle formation. © 2008 Elsevier Ltd. All rights reserved.
format	JOUR
author	Romanutti, C. Bruttomesso, A.C. Castilla, V. Bisceglia, J.A. Galagovsky, L.R. Wachsman, M.B.
author_facet	Romanutti, C. Bruttomesso, A.C. Castilla, V. Bisceglia, J.A. Galagovsky, L.R. Wachsman, M.B.
author_sort	Romanutti, C.
title	In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus
title_short	In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus
title_full	In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus
title_fullStr	In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus
title_full_unstemmed	In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus
title_sort	in vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus
url	http://hdl.handle.net/20.500.12110/paper_10900233_v182_n2_p327_Romanutti
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In vitro antiviral activity of dehydroepiandrosterone and its synthetic derivatives against vesicular stomatitis virus

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