FRET imaging

Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a revised photophysical framework for the phenomenon and provide a systematic catalog of FRET te...

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Detalles Bibliográficos
Autores principales: Jares-Erijman, E.A., Jovin, T.M.
Formato: JOUR
Materias:
Cells
Fluorescence
Microscopic examination
Photophysics
Imaging techniques
algorithm
calculation
cell
conformation
fluorescence resonance energy transfer imaging
imaging system
light
optics
phenomenology
priority journal
review
Fluorescence Resonance Energy Transfer
Fluorescent Dyes
Microscopy, Fluorescence
Models, Chemical
Proteins
Technology Assessment, Biomedical
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10870156_v21_n11_p1387_JaresErijman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a revised photophysical framework for the phenomenon and provide a systematic catalog of FRET techniques adapted to imaging systems, including new approaches proposed as suitable prospects for implementation. Applications extending from a single molecule to live cells will benefit from multidimensional microscopy techniques, particularly those adapted for optical sectioning and incorporating new algorithms for resolving the component contributions to images of complex molecular systems.

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