Effects of radioiodination on hormone binding ability

This paper presents the effects of 125I labelling on human chorionic gonadotropin binding ability. The results obtained showed a marked difference in the half-life of the specific radioactivity of the radiolabelled hormone comparing the one obtained by "self-displacement" analysis and that...

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Autores principales: Radicella, J.P., Calvo, J.C., Charreau, E.H.
Formato: JOUR
Materias:
radioisotope
chorionic gonadotropin i 125
endocrine system
hormone binding
human
human cell
methodology
priority journal
radioiodination
Animal
Binding Sites
Chorionic Gonadotropin
Chromatography, Gel
Half-Life
In Vitro
Iodine Radioisotopes
Kinetics
Male
Molecular Weight
Rats
Testis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10799893_v5_n5-6_p349_Radicella
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_10799893_v5_n5-6_p349_Radicella
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spelling todo:paper_10799893_v5_n5-6_p349_Radicella2023-10-03T16:03:44Z Effects of radioiodination on hormone binding ability Radicella, J.P. Calvo, J.C. Charreau, E.H. radioisotope chorionic gonadotropin i 125 endocrine system hormone binding human human cell methodology priority journal radioiodination Animal Binding Sites Chorionic Gonadotropin Chromatography, Gel Half-Life In Vitro Iodine Radioisotopes Kinetics Male Molecular Weight Rats Testis This paper presents the effects of 125I labelling on human chorionic gonadotropin binding ability. The results obtained showed a marked difference in the half-life of the specific radioactivity of the radiolabelled hormone comparing the one obtained by "self-displacement" analysis and that calculated considering the half-life of the radioactive isotope (60 days for 125I). The decrease in the hormone half-life (t1/2= 3.10 days) was demonstrated by binding experiments performed at various days after hormone labelling. The affinity constant (Ka) and the number of binding sites obtained after Scatchard analysis using rat testes as receptor source, remained constant when the "self-displacement" specific radioactivity was used while they varied significantly if the 60 days half-life was considered for the data processing. The importance of the labelled hormone degradation as a possible cause of the decrease in the binding ability is also described. © 1985 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. Fil:Radicella, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10799893_v5_n5-6_p349_Radicella
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic radioisotope
chorionic gonadotropin i 125
endocrine system
hormone binding
human
human cell
methodology
priority journal
radioiodination
Animal
Binding Sites
Chorionic Gonadotropin
Chromatography, Gel
Half-Life
In Vitro
Iodine Radioisotopes
Kinetics
Male
Molecular Weight
Rats
Testis
spellingShingle radioisotope
chorionic gonadotropin i 125
endocrine system
hormone binding
human
human cell
methodology
priority journal
radioiodination
Animal
Binding Sites
Chorionic Gonadotropin
Chromatography, Gel
Half-Life
In Vitro
Iodine Radioisotopes
Kinetics
Male
Molecular Weight
Rats
Testis
Radicella, J.P.
Calvo, J.C.
Charreau, E.H.
Effects of radioiodination on hormone binding ability
topic_facet radioisotope
chorionic gonadotropin i 125
endocrine system
hormone binding
human
human cell
methodology
priority journal
radioiodination
Animal
Binding Sites
Chorionic Gonadotropin
Chromatography, Gel
Half-Life
In Vitro
Iodine Radioisotopes
Kinetics
Male
Molecular Weight
Rats
Testis
description This paper presents the effects of 125I labelling on human chorionic gonadotropin binding ability. The results obtained showed a marked difference in the half-life of the specific radioactivity of the radiolabelled hormone comparing the one obtained by "self-displacement" analysis and that calculated considering the half-life of the radioactive isotope (60 days for 125I). The decrease in the hormone half-life (t1/2= 3.10 days) was demonstrated by binding experiments performed at various days after hormone labelling. The affinity constant (Ka) and the number of binding sites obtained after Scatchard analysis using rat testes as receptor source, remained constant when the "self-displacement" specific radioactivity was used while they varied significantly if the 60 days half-life was considered for the data processing. The importance of the labelled hormone degradation as a possible cause of the decrease in the binding ability is also described. © 1985 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
format JOUR
author Radicella, J.P.
Calvo, J.C.
Charreau, E.H.
author_facet Radicella, J.P.
Calvo, J.C.
Charreau, E.H.
author_sort Radicella, J.P.
title Effects of radioiodination on hormone binding ability
title_short Effects of radioiodination on hormone binding ability
title_full Effects of radioiodination on hormone binding ability
title_fullStr Effects of radioiodination on hormone binding ability
title_full_unstemmed Effects of radioiodination on hormone binding ability
title_sort effects of radioiodination on hormone binding ability
url http://hdl.handle.net/20.500.12110/paper_10799893_v5_n5-6_p349_Radicella
work_keys_str_mv AT radicellajp effectsofradioiodinationonhormonebindingability
AT calvojc effectsofradioiodinationonhormonebindingability
AT charreaueh effectsofradioiodinationonhormonebindingability
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