MALDI-MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation
In many amphibians, the granular glands can be grouped in special regions forming macroglands. This is the case of toads, characterized by the presence of a pair of parotoid macroglands, strategically located to give protection by poison release in case of attacks. The product secreted consists of a...
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todo:paper_10765174_v53_n6_p465_Petroselli2023-10-03T16:03:27Z MALDI-MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation Petroselli, G. Raices, M. Jungblut, L.D. Pozzi, A.G. Erra-Balsells, R. bufadienolides fingerprint MALDI-MS parotoid secretion Rhinella arenarum Amines Desorption Electrospray ionization Esters Mass spectrometry Physiology Proteins bufadienolides fingerprint MALDI-MS parotoid secretion Rhinella arenarum Ultraviolet lasers argininylbufadienolide ester bufadienolide derivative ester unclassified drug arginine bufadienolide derivative ester adult age Anura Article chemical composition controlled study desorption electrospray mass spectrometry female male matrix-assisted laser desorption-ionization mass spectrometry nonhuman parotid gland priority journal Rhinella arenarum saliva analysis salivation seasonal variation sex difference tandem mass spectrometry ultraviolet radiation analogs and derivatives animal Chordata growth, development and aging high performance liquid chromatography matrix-assisted laser desorption-ionization mass spectrometry metabolism parotid gland physiology principal component analysis procedures season secretion (process) Animals Arginine Bufanolides Chordata Chromatography, High Pressure Liquid Esters Female Male Parotid Gland Principal Component Analysis Seasons Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tandem Mass Spectrometry In many amphibians, the granular glands can be grouped in special regions forming macroglands. This is the case of toads, characterized by the presence of a pair of parotoid macroglands, strategically located to give protection by poison release in case of attacks. The product secreted consists of a wide variety of chemical compounds including proteins, peptides, biogenic amines, toxic steroidal bufadienolides, and various alkaloids, depending on the species. In this work, using Rhinella arenarum, we have performed, for the first time, the matrix assisted-ultraviolet laser desorption/ionization mass spectrometry and tandem mass spectrometry characterization of the components of the secretion used as crude material, just suspended in MeOH (or MeCN). The crude sample as a whole (whole suspension) was spotted on the matrix assisted-ultraviolet laser desorption plate for analysis. Electrospray ionization-Orbitrap was used for cross-checking experiments. The pattern of signals obtained at m/z ranges 600 to 800 and 1200 to 1600 could be assigned as the argininyl bufadienolide esters fingerprint characteristic of female and male. Variation patterns for gender (female, male), age (non-reproductive, reproductive), and season (non-reproductive, reproductive) are described. Copyright © 2018 John Wiley & Sons, Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10765174_v53_n6_p465_Petroselli |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
bufadienolides fingerprint MALDI-MS parotoid secretion Rhinella arenarum Amines Desorption Electrospray ionization Esters Mass spectrometry Physiology Proteins bufadienolides fingerprint MALDI-MS parotoid secretion Rhinella arenarum Ultraviolet lasers argininylbufadienolide ester bufadienolide derivative ester unclassified drug arginine bufadienolide derivative ester adult age Anura Article chemical composition controlled study desorption electrospray mass spectrometry female male matrix-assisted laser desorption-ionization mass spectrometry nonhuman parotid gland priority journal Rhinella arenarum saliva analysis salivation seasonal variation sex difference tandem mass spectrometry ultraviolet radiation analogs and derivatives animal Chordata growth, development and aging high performance liquid chromatography matrix-assisted laser desorption-ionization mass spectrometry metabolism parotid gland physiology principal component analysis procedures season secretion (process) Animals Arginine Bufanolides Chordata Chromatography, High Pressure Liquid Esters Female Male Parotid Gland Principal Component Analysis Seasons Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tandem Mass Spectrometry |
spellingShingle |
bufadienolides fingerprint MALDI-MS parotoid secretion Rhinella arenarum Amines Desorption Electrospray ionization Esters Mass spectrometry Physiology Proteins bufadienolides fingerprint MALDI-MS parotoid secretion Rhinella arenarum Ultraviolet lasers argininylbufadienolide ester bufadienolide derivative ester unclassified drug arginine bufadienolide derivative ester adult age Anura Article chemical composition controlled study desorption electrospray mass spectrometry female male matrix-assisted laser desorption-ionization mass spectrometry nonhuman parotid gland priority journal Rhinella arenarum saliva analysis salivation seasonal variation sex difference tandem mass spectrometry ultraviolet radiation analogs and derivatives animal Chordata growth, development and aging high performance liquid chromatography matrix-assisted laser desorption-ionization mass spectrometry metabolism parotid gland physiology principal component analysis procedures season secretion (process) Animals Arginine Bufanolides Chordata Chromatography, High Pressure Liquid Esters Female Male Parotid Gland Principal Component Analysis Seasons Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tandem Mass Spectrometry Petroselli, G. Raices, M. Jungblut, L.D. Pozzi, A.G. Erra-Balsells, R. MALDI-MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation |
topic_facet |
bufadienolides fingerprint MALDI-MS parotoid secretion Rhinella arenarum Amines Desorption Electrospray ionization Esters Mass spectrometry Physiology Proteins bufadienolides fingerprint MALDI-MS parotoid secretion Rhinella arenarum Ultraviolet lasers argininylbufadienolide ester bufadienolide derivative ester unclassified drug arginine bufadienolide derivative ester adult age Anura Article chemical composition controlled study desorption electrospray mass spectrometry female male matrix-assisted laser desorption-ionization mass spectrometry nonhuman parotid gland priority journal Rhinella arenarum saliva analysis salivation seasonal variation sex difference tandem mass spectrometry ultraviolet radiation analogs and derivatives animal Chordata growth, development and aging high performance liquid chromatography matrix-assisted laser desorption-ionization mass spectrometry metabolism parotid gland physiology principal component analysis procedures season secretion (process) Animals Arginine Bufanolides Chordata Chromatography, High Pressure Liquid Esters Female Male Parotid Gland Principal Component Analysis Seasons Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tandem Mass Spectrometry |
description |
In many amphibians, the granular glands can be grouped in special regions forming macroglands. This is the case of toads, characterized by the presence of a pair of parotoid macroglands, strategically located to give protection by poison release in case of attacks. The product secreted consists of a wide variety of chemical compounds including proteins, peptides, biogenic amines, toxic steroidal bufadienolides, and various alkaloids, depending on the species. In this work, using Rhinella arenarum, we have performed, for the first time, the matrix assisted-ultraviolet laser desorption/ionization mass spectrometry and tandem mass spectrometry characterization of the components of the secretion used as crude material, just suspended in MeOH (or MeCN). The crude sample as a whole (whole suspension) was spotted on the matrix assisted-ultraviolet laser desorption plate for analysis. Electrospray ionization-Orbitrap was used for cross-checking experiments. The pattern of signals obtained at m/z ranges 600 to 800 and 1200 to 1600 could be assigned as the argininyl bufadienolide esters fingerprint characteristic of female and male. Variation patterns for gender (female, male), age (non-reproductive, reproductive), and season (non-reproductive, reproductive) are described. Copyright © 2018 John Wiley & Sons, Ltd. |
format |
JOUR |
author |
Petroselli, G. Raices, M. Jungblut, L.D. Pozzi, A.G. Erra-Balsells, R. |
author_facet |
Petroselli, G. Raices, M. Jungblut, L.D. Pozzi, A.G. Erra-Balsells, R. |
author_sort |
Petroselli, G. |
title |
MALDI-MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation |
title_short |
MALDI-MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation |
title_full |
MALDI-MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation |
title_fullStr |
MALDI-MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation |
title_full_unstemmed |
MALDI-MS argininyl bufadienolide esters fingerprint from parotoid gland secretions of Rhinella arenarum: Age, gender, and seasonal variation |
title_sort |
maldi-ms argininyl bufadienolide esters fingerprint from parotoid gland secretions of rhinella arenarum: age, gender, and seasonal variation |
url |
http://hdl.handle.net/20.500.12110/paper_10765174_v53_n6_p465_Petroselli |
work_keys_str_mv |
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