Direct analysis by electrospray ionization tandem mass spectrometry of mixtures of phosphatidyldiacylglycerols from Lactobacillus
Electrospray ionization followed by collision-induced dissociation in a quadrupole ion trap mass spectrometer of mixtures of deprotonated phosphatidyldiacylglycerols afforded a group of three diagnostic ions of convenient abundance for each phosphatidyldiacylglycerol (PG) present in the mixture. Thu...
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10765174_v35_n12_p1452_Cabrera |
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todo:paper_10765174_v35_n12_p1452_Cabrera2023-10-03T16:03:22Z Direct analysis by electrospray ionization tandem mass spectrometry of mixtures of phosphatidyldiacylglycerols from Lactobacillus Cabrera, G.M. Fern´andez Murga, M.L. Font de Valdez, G. Seldes, A.M. Collision-induced dissociation Electrospray ionization mass spectrometry Phosphatidyldiacylglycerols Quadrupole ion trap mass spectrometry Sn-1 and sn-2 acyl substitution diacylglycerol derivative phosphatidyldiglyceride unclassified drug article chemical analysis controlled study dissociation extraction fast atom bombardment mass spectrometry ionization Lactobacillus acidophilus mass spectrometry nonhuman priority journal proton transport Electrospray ionization followed by collision-induced dissociation in a quadrupole ion trap mass spectrometer of mixtures of deprotonated phosphatidyldiacylglycerols afforded a group of three diagnostic ions of convenient abundance for each phosphatidyldiacylglycerol (PG) present in the mixture. Thus, it was possible to determine unmistakably the identity and substitution positions (sn-1 or sn-2) for both acyl groups of each PG present in the mixture. The method also allows the study of isomeric mixtures of PG and mixtures containing minor amounts of some PG from crude extracts of Lactobacillus acidophillus. The present results improve those of previous studies using fast atom bombardment and electrospray ionization tanden mass spectrometry, in which it was reported that it was possible to differentiate the identity and position of the sn-2 acyl substituent only by the presence of one ion, with variable abundance. Copyright © 2000 John Wiley & Sons, Ltd. Fil:Cabrera, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Seldes, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10765174_v35_n12_p1452_Cabrera |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Collision-induced dissociation Electrospray ionization mass spectrometry Phosphatidyldiacylglycerols Quadrupole ion trap mass spectrometry Sn-1 and sn-2 acyl substitution diacylglycerol derivative phosphatidyldiglyceride unclassified drug article chemical analysis controlled study dissociation extraction fast atom bombardment mass spectrometry ionization Lactobacillus acidophilus mass spectrometry nonhuman priority journal proton transport |
| spellingShingle |
Collision-induced dissociation Electrospray ionization mass spectrometry Phosphatidyldiacylglycerols Quadrupole ion trap mass spectrometry Sn-1 and sn-2 acyl substitution diacylglycerol derivative phosphatidyldiglyceride unclassified drug article chemical analysis controlled study dissociation extraction fast atom bombardment mass spectrometry ionization Lactobacillus acidophilus mass spectrometry nonhuman priority journal proton transport Cabrera, G.M. Fern´andez Murga, M.L. Font de Valdez, G. Seldes, A.M. Direct analysis by electrospray ionization tandem mass spectrometry of mixtures of phosphatidyldiacylglycerols from Lactobacillus |
| topic_facet |
Collision-induced dissociation Electrospray ionization mass spectrometry Phosphatidyldiacylglycerols Quadrupole ion trap mass spectrometry Sn-1 and sn-2 acyl substitution diacylglycerol derivative phosphatidyldiglyceride unclassified drug article chemical analysis controlled study dissociation extraction fast atom bombardment mass spectrometry ionization Lactobacillus acidophilus mass spectrometry nonhuman priority journal proton transport |
| description |
Electrospray ionization followed by collision-induced dissociation in a quadrupole ion trap mass spectrometer of mixtures of deprotonated phosphatidyldiacylglycerols afforded a group of three diagnostic ions of convenient abundance for each phosphatidyldiacylglycerol (PG) present in the mixture. Thus, it was possible to determine unmistakably the identity and substitution positions (sn-1 or sn-2) for both acyl groups of each PG present in the mixture. The method also allows the study of isomeric mixtures of PG and mixtures containing minor amounts of some PG from crude extracts of Lactobacillus acidophillus. The present results improve those of previous studies using fast atom bombardment and electrospray ionization tanden mass spectrometry, in which it was reported that it was possible to differentiate the identity and position of the sn-2 acyl substituent only by the presence of one ion, with variable abundance. Copyright © 2000 John Wiley & Sons, Ltd. |
| format |
JOUR |
| author |
Cabrera, G.M. Fern´andez Murga, M.L. Font de Valdez, G. Seldes, A.M. |
| author_facet |
Cabrera, G.M. Fern´andez Murga, M.L. Font de Valdez, G. Seldes, A.M. |
| author_sort |
Cabrera, G.M. |
| title |
Direct analysis by electrospray ionization tandem mass spectrometry of mixtures of phosphatidyldiacylglycerols from Lactobacillus |
| title_short |
Direct analysis by electrospray ionization tandem mass spectrometry of mixtures of phosphatidyldiacylglycerols from Lactobacillus |
| title_full |
Direct analysis by electrospray ionization tandem mass spectrometry of mixtures of phosphatidyldiacylglycerols from Lactobacillus |
| title_fullStr |
Direct analysis by electrospray ionization tandem mass spectrometry of mixtures of phosphatidyldiacylglycerols from Lactobacillus |
| title_full_unstemmed |
Direct analysis by electrospray ionization tandem mass spectrometry of mixtures of phosphatidyldiacylglycerols from Lactobacillus |
| title_sort |
direct analysis by electrospray ionization tandem mass spectrometry of mixtures of phosphatidyldiacylglycerols from lactobacillus |
| url |
http://hdl.handle.net/20.500.12110/paper_10765174_v35_n12_p1452_Cabrera |
| work_keys_str_mv |
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