Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase
The in vivo effect of the known herbicide, paraquat, on both hepatic oxidative stress and heme metabolism was studied. A marked increase in lipid peroxidation and a decrease in reduced glutathione (GSH) content were observed 1 h after paraquat administration. The activity of liver antioxidant enzyme...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10715762_v36_n6_p633_Noriega |
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todo:paper_10715762_v36_n6_p633_Noriega2023-10-03T16:02:39Z Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase Noriega, G.O. Gonzales, S. Tomaro, M.L. Del, A.M. Batlle, C. δ-aminolevulinic acid synthase Antioxidant enzymes Heme metabolism Heme oxygenase-1 Oxidative stress Paraquat 5 aminolevulinate synthase alpha tocopherol antioxidant catalase glutathione glutathione peroxidase heme heme oxygenase 1 herbicide lipid liver enzyme paraquat porphobilinogen synthase superoxide dismutase thiobarbituric acid reactive substance article biosynthesis body weight controlled study degradation enzyme activity enzyme assay enzyme inhibition in vivo study lipid peroxidation liver metabolism nonhuman oxidative stress porphyrin metabolism rat 5-Aminolevulinate Synthetase alpha-Tocopherol Animals Antioxidants Enzymes Female Glutathione Heme Heme Oxygenase (Decyclizing) Heme Oxygenase-1 Herbicides Liver Oxidative Stress Paraquat Rats Rats, Wistar Thiobarbituric Acid Reactive Substances The in vivo effect of the known herbicide, paraquat, on both hepatic oxidative stress and heme metabolism was studied. A marked increase in lipid peroxidation and a decrease in reduced glutathione (GSH) content were observed 1 h after paraquat administration. The activity of liver antioxidant enzymes, superoxide dismutase, catalase and glutathione peroxidase was decreased 3 h after paraquat injection. Heme oxygenase-1 induction started 9 h after treatment, peaking at 15 h. δ-aminolevulinic acid synthase induction occurred once heme oxygenase had been enhanced, reaching its maximum (1.5-fold of control) at 16 h. δ-aminolevulinic acid dehydratase activity was 40% inhibited at 3 h showing a profile similar to that of GSH, while porphobilinogenase activity was not modified along the whole period of the assay. Administration of α-tocopherol (35 mmol/kg body weight) 2 h before paraquat treatment entirely prevented the increase in thiobarbituric acid reactive substances (TBARS) content, the decrease in GSH levels as well as heme oxygenase-1 and δ-aminolevulinic acid synthase induction. This study shows that oxidative stress produced by paraquat leads to an increase in δ-aminolevulinic acid synthase and heme oxygenase-1 activities, indicating that the herbicide affects both heme biosynthesis and degradation. Fil:Noriega, G.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10715762_v36_n6_p633_Noriega |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
δ-aminolevulinic acid synthase Antioxidant enzymes Heme metabolism Heme oxygenase-1 Oxidative stress Paraquat 5 aminolevulinate synthase alpha tocopherol antioxidant catalase glutathione glutathione peroxidase heme heme oxygenase 1 herbicide lipid liver enzyme paraquat porphobilinogen synthase superoxide dismutase thiobarbituric acid reactive substance article biosynthesis body weight controlled study degradation enzyme activity enzyme assay enzyme inhibition in vivo study lipid peroxidation liver metabolism nonhuman oxidative stress porphyrin metabolism rat 5-Aminolevulinate Synthetase alpha-Tocopherol Animals Antioxidants Enzymes Female Glutathione Heme Heme Oxygenase (Decyclizing) Heme Oxygenase-1 Herbicides Liver Oxidative Stress Paraquat Rats Rats, Wistar Thiobarbituric Acid Reactive Substances |
| spellingShingle |
δ-aminolevulinic acid synthase Antioxidant enzymes Heme metabolism Heme oxygenase-1 Oxidative stress Paraquat 5 aminolevulinate synthase alpha tocopherol antioxidant catalase glutathione glutathione peroxidase heme heme oxygenase 1 herbicide lipid liver enzyme paraquat porphobilinogen synthase superoxide dismutase thiobarbituric acid reactive substance article biosynthesis body weight controlled study degradation enzyme activity enzyme assay enzyme inhibition in vivo study lipid peroxidation liver metabolism nonhuman oxidative stress porphyrin metabolism rat 5-Aminolevulinate Synthetase alpha-Tocopherol Animals Antioxidants Enzymes Female Glutathione Heme Heme Oxygenase (Decyclizing) Heme Oxygenase-1 Herbicides Liver Oxidative Stress Paraquat Rats Rats, Wistar Thiobarbituric Acid Reactive Substances Noriega, G.O. Gonzales, S. Tomaro, M.L. Del, A.M. Batlle, C. Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase |
| topic_facet |
δ-aminolevulinic acid synthase Antioxidant enzymes Heme metabolism Heme oxygenase-1 Oxidative stress Paraquat 5 aminolevulinate synthase alpha tocopherol antioxidant catalase glutathione glutathione peroxidase heme heme oxygenase 1 herbicide lipid liver enzyme paraquat porphobilinogen synthase superoxide dismutase thiobarbituric acid reactive substance article biosynthesis body weight controlled study degradation enzyme activity enzyme assay enzyme inhibition in vivo study lipid peroxidation liver metabolism nonhuman oxidative stress porphyrin metabolism rat 5-Aminolevulinate Synthetase alpha-Tocopherol Animals Antioxidants Enzymes Female Glutathione Heme Heme Oxygenase (Decyclizing) Heme Oxygenase-1 Herbicides Liver Oxidative Stress Paraquat Rats Rats, Wistar Thiobarbituric Acid Reactive Substances |
| description |
The in vivo effect of the known herbicide, paraquat, on both hepatic oxidative stress and heme metabolism was studied. A marked increase in lipid peroxidation and a decrease in reduced glutathione (GSH) content were observed 1 h after paraquat administration. The activity of liver antioxidant enzymes, superoxide dismutase, catalase and glutathione peroxidase was decreased 3 h after paraquat injection. Heme oxygenase-1 induction started 9 h after treatment, peaking at 15 h. δ-aminolevulinic acid synthase induction occurred once heme oxygenase had been enhanced, reaching its maximum (1.5-fold of control) at 16 h. δ-aminolevulinic acid dehydratase activity was 40% inhibited at 3 h showing a profile similar to that of GSH, while porphobilinogenase activity was not modified along the whole period of the assay. Administration of α-tocopherol (35 mmol/kg body weight) 2 h before paraquat treatment entirely prevented the increase in thiobarbituric acid reactive substances (TBARS) content, the decrease in GSH levels as well as heme oxygenase-1 and δ-aminolevulinic acid synthase induction. This study shows that oxidative stress produced by paraquat leads to an increase in δ-aminolevulinic acid synthase and heme oxygenase-1 activities, indicating that the herbicide affects both heme biosynthesis and degradation. |
| format |
JOUR |
| author |
Noriega, G.O. Gonzales, S. Tomaro, M.L. Del, A.M. Batlle, C. |
| author_facet |
Noriega, G.O. Gonzales, S. Tomaro, M.L. Del, A.M. Batlle, C. |
| author_sort |
Noriega, G.O. |
| title |
Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase |
| title_short |
Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase |
| title_full |
Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase |
| title_fullStr |
Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase |
| title_full_unstemmed |
Paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase |
| title_sort |
paraquat-generated oxidative stress in rat liver induces heme oxygenase-1 and aminolevulinic acid synthase |
| url |
http://hdl.handle.net/20.500.12110/paper_10715762_v36_n6_p633_Noriega |
| work_keys_str_mv |
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| _version_ |
1782026658006433792 |