Steroidogenesis in immature chicken ovary. Hormonal stimulation of adenylyl cyclase system by luteinizing hormone and β-adrenergic agonists

The presence of a hormonally responsive adenylyl cyclase in the immature chicken ovary was investigated. We found that there was a highly significant difference (P< 0. 05) between basal and LH and catecholamine activatable activities. In addition, the basal activity was stimulated by NaF, forskol...

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Detalles Bibliográficos
Autores principales: Gonzalez, C.B., Cantore, M.L., Passeron, S.
Formato: JOUR
Materias:
adenylate cyclase
adrenalin
beta adrenergic receptor stimulating agent
catecholamine
cyclic amp
fluoride sodium
forskolin
guanylylimidodiphosphate
isoprenaline
luteinizing hormone
progesterone
propranolol
animal tissue
article
chicken
dose response
drug antagonism
drug mixture
drug potentiation
enzyme activation
enzyme activity
enzyme inhibition
hormonal regulation
nonhuman
ovary development
progesterone synthesis
second messenger
signal transduction
steroidogenesis
tissue homogenate
Adenylate Cyclase
Animal
Chickens
Cyclic AMP
Enzyme Activation
Epinephrine
Female
Forskolin
Guanylyl Imidodiphosphate
Isoproterenol
Luteinizing Hormone
Ovary
Progesterone
Propranolol
Sodium Fluoride
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10656995_v18_n2_p103_Gonzalez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The presence of a hormonally responsive adenylyl cyclase in the immature chicken ovary was investigated. We found that there was a highly significant difference (P< 0. 05) between basal and LH and catecholamine activatable activities. In addition, the basal activity was stimulated by NaF, forskolin and the non-hydrolyzable GTP analogue guanosine-5′-(β, gamma;-imido)-triphosphate (GMPP(NH)P. The action of catecholamines on cyclic AMP and progesterone production was also investigated and compared to that of LH. The stimulatory effect of isoproterenol on cyclic AMP and progesterone production was significantly higher (P< 0.05) than that of LH. The β-adrenergic antagonist propranolol caused complete inhibition of the stimulatory action of catecholamines. Progesterone accumulation induced by LH or isoproterenol was synergistically augmentated by the simultaneous presence of both inducers. © 1994 Academic Press. All rights reserved.

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