Steroidogenesis in immature chicken ovary. Hormonal stimulation of adenylyl cyclase system by luteinizing hormone and β-adrenergic agonists
The presence of a hormonally responsive adenylyl cyclase in the immature chicken ovary was investigated. We found that there was a highly significant difference (P< 0. 05) between basal and LH and catecholamine activatable activities. In addition, the basal activity was stimulated by NaF, forskol...
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todo:paper_10656995_v18_n2_p103_Gonzalez2023-10-03T16:02:04Z Steroidogenesis in immature chicken ovary. Hormonal stimulation of adenylyl cyclase system by luteinizing hormone and β-adrenergic agonists Gonzalez, C.B. Cantore, M.L. Passeron, S. adenylate cyclase adrenalin beta adrenergic receptor stimulating agent catecholamine cyclic amp fluoride sodium forskolin guanylylimidodiphosphate isoprenaline luteinizing hormone progesterone propranolol animal tissue article chicken dose response drug antagonism drug mixture drug potentiation enzyme activation enzyme activity enzyme inhibition hormonal regulation nonhuman ovary development progesterone synthesis second messenger signal transduction steroidogenesis tissue homogenate Adenylate Cyclase Animal Chickens Cyclic AMP Enzyme Activation Epinephrine Female Forskolin Guanylyl Imidodiphosphate Isoproterenol Luteinizing Hormone Ovary Progesterone Propranolol Sodium Fluoride Support, Non-U.S. Gov't The presence of a hormonally responsive adenylyl cyclase in the immature chicken ovary was investigated. We found that there was a highly significant difference (P< 0. 05) between basal and LH and catecholamine activatable activities. In addition, the basal activity was stimulated by NaF, forskolin and the non-hydrolyzable GTP analogue guanosine-5′-(β, gamma;-imido)-triphosphate (GMPP(NH)P. The action of catecholamines on cyclic AMP and progesterone production was also investigated and compared to that of LH. The stimulatory effect of isoproterenol on cyclic AMP and progesterone production was significantly higher (P< 0.05) than that of LH. The β-adrenergic antagonist propranolol caused complete inhibition of the stimulatory action of catecholamines. Progesterone accumulation induced by LH or isoproterenol was synergistically augmentated by the simultaneous presence of both inducers. © 1994 Academic Press. All rights reserved. Fil:Gonzalez, C.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cantore, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10656995_v18_n2_p103_Gonzalez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adenylate cyclase adrenalin beta adrenergic receptor stimulating agent catecholamine cyclic amp fluoride sodium forskolin guanylylimidodiphosphate isoprenaline luteinizing hormone progesterone propranolol animal tissue article chicken dose response drug antagonism drug mixture drug potentiation enzyme activation enzyme activity enzyme inhibition hormonal regulation nonhuman ovary development progesterone synthesis second messenger signal transduction steroidogenesis tissue homogenate Adenylate Cyclase Animal Chickens Cyclic AMP Enzyme Activation Epinephrine Female Forskolin Guanylyl Imidodiphosphate Isoproterenol Luteinizing Hormone Ovary Progesterone Propranolol Sodium Fluoride Support, Non-U.S. Gov't |
| spellingShingle |
adenylate cyclase adrenalin beta adrenergic receptor stimulating agent catecholamine cyclic amp fluoride sodium forskolin guanylylimidodiphosphate isoprenaline luteinizing hormone progesterone propranolol animal tissue article chicken dose response drug antagonism drug mixture drug potentiation enzyme activation enzyme activity enzyme inhibition hormonal regulation nonhuman ovary development progesterone synthesis second messenger signal transduction steroidogenesis tissue homogenate Adenylate Cyclase Animal Chickens Cyclic AMP Enzyme Activation Epinephrine Female Forskolin Guanylyl Imidodiphosphate Isoproterenol Luteinizing Hormone Ovary Progesterone Propranolol Sodium Fluoride Support, Non-U.S. Gov't Gonzalez, C.B. Cantore, M.L. Passeron, S. Steroidogenesis in immature chicken ovary. Hormonal stimulation of adenylyl cyclase system by luteinizing hormone and β-adrenergic agonists |
| topic_facet |
adenylate cyclase adrenalin beta adrenergic receptor stimulating agent catecholamine cyclic amp fluoride sodium forskolin guanylylimidodiphosphate isoprenaline luteinizing hormone progesterone propranolol animal tissue article chicken dose response drug antagonism drug mixture drug potentiation enzyme activation enzyme activity enzyme inhibition hormonal regulation nonhuman ovary development progesterone synthesis second messenger signal transduction steroidogenesis tissue homogenate Adenylate Cyclase Animal Chickens Cyclic AMP Enzyme Activation Epinephrine Female Forskolin Guanylyl Imidodiphosphate Isoproterenol Luteinizing Hormone Ovary Progesterone Propranolol Sodium Fluoride Support, Non-U.S. Gov't |
| description |
The presence of a hormonally responsive adenylyl cyclase in the immature chicken ovary was investigated. We found that there was a highly significant difference (P< 0. 05) between basal and LH and catecholamine activatable activities. In addition, the basal activity was stimulated by NaF, forskolin and the non-hydrolyzable GTP analogue guanosine-5′-(β, gamma;-imido)-triphosphate (GMPP(NH)P. The action of catecholamines on cyclic AMP and progesterone production was also investigated and compared to that of LH. The stimulatory effect of isoproterenol on cyclic AMP and progesterone production was significantly higher (P< 0.05) than that of LH. The β-adrenergic antagonist propranolol caused complete inhibition of the stimulatory action of catecholamines. Progesterone accumulation induced by LH or isoproterenol was synergistically augmentated by the simultaneous presence of both inducers. © 1994 Academic Press. All rights reserved. |
| format |
JOUR |
| author |
Gonzalez, C.B. Cantore, M.L. Passeron, S. |
| author_facet |
Gonzalez, C.B. Cantore, M.L. Passeron, S. |
| author_sort |
Gonzalez, C.B. |
| title |
Steroidogenesis in immature chicken ovary. Hormonal stimulation of adenylyl cyclase system by luteinizing hormone and β-adrenergic agonists |
| title_short |
Steroidogenesis in immature chicken ovary. Hormonal stimulation of adenylyl cyclase system by luteinizing hormone and β-adrenergic agonists |
| title_full |
Steroidogenesis in immature chicken ovary. Hormonal stimulation of adenylyl cyclase system by luteinizing hormone and β-adrenergic agonists |
| title_fullStr |
Steroidogenesis in immature chicken ovary. Hormonal stimulation of adenylyl cyclase system by luteinizing hormone and β-adrenergic agonists |
| title_full_unstemmed |
Steroidogenesis in immature chicken ovary. Hormonal stimulation of adenylyl cyclase system by luteinizing hormone and β-adrenergic agonists |
| title_sort |
steroidogenesis in immature chicken ovary. hormonal stimulation of adenylyl cyclase system by luteinizing hormone and β-adrenergic agonists |
| url |
http://hdl.handle.net/20.500.12110/paper_10656995_v18_n2_p103_Gonzalez |
| work_keys_str_mv |
AT gonzalezcb steroidogenesisinimmaturechickenovaryhormonalstimulationofadenylylcyclasesystembyluteinizinghormoneandbadrenergicagonists AT cantoreml steroidogenesisinimmaturechickenovaryhormonalstimulationofadenylylcyclasesystembyluteinizinghormoneandbadrenergicagonists AT passerons steroidogenesisinimmaturechickenovaryhormonalstimulationofadenylylcyclasesystembyluteinizinghormoneandbadrenergicagonists |
| _version_ |
1807323481924173824 |