Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA

We describe a recombineering-based method for the genetic manipulation of lytically replicating bacteriophages, focusing on mycobacteriophages. The approach utilizes recombineering-proficient strains of Mycobacterium smegmatis and employs a cotransformation strategy with purified phage genomic DNA a...

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Detalles Bibliográficos
Autores principales:	Marinelli, L.J., Piuri, M., Hatfull, G.F.
Formato:	SER
Materias:	BRED Electroporation Mycobacteria Mycobacteriophage Recombineering bacteriophage DNA genomic DNA oligonucleotide Rac protein recombinant protein RecT protein bacteriophage recombineering of electroporated DNA bacterium culture electroporation genetic engineering genetic manipulation genetic recombination methodology mutant mycobacteriophage Mycobacterium smegmatis natural science nonhuman polymerase chain reaction prophage recombineering
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_10643745_v1898_n_p69_Marinelli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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