Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA
We describe a recombineering-based method for the genetic manipulation of lytically replicating bacteriophages, focusing on mycobacteriophages. The approach utilizes recombineering-proficient strains of Mycobacterium smegmatis and employs a cotransformation strategy with purified phage genomic DNA a...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10643745_v1898_n_p69_Marinelli |
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todo:paper_10643745_v1898_n_p69_Marinelli2023-10-03T16:01:58Z Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA Marinelli, L.J. Piuri, M. Hatfull, G.F. BRED Electroporation Mycobacteria Mycobacteriophage Recombineering bacteriophage DNA genomic DNA oligonucleotide Rac protein recombinant protein RecT protein bacteriophage recombineering of electroporated DNA bacterium culture electroporation genetic engineering genetic manipulation genetic recombination methodology mutant mycobacteriophage Mycobacterium smegmatis natural science nonhuman polymerase chain reaction prophage recombineering We describe a recombineering-based method for the genetic manipulation of lytically replicating bacteriophages, focusing on mycobacteriophages. The approach utilizes recombineering-proficient strains of Mycobacterium smegmatis and employs a cotransformation strategy with purified phage genomic DNA and a mutagenic substrate, which selects for only those cells that are competent to take up DNA. The cotransformation method, combined with the high rates of recombination obtained in M. smegmatis recombineering strains, allows for the efficient and rapid generation of bacteriophage mutants. © 2019, Springer Science+Business Media, LLC, part of Springer Nature. SER info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10643745_v1898_n_p69_Marinelli |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
BRED Electroporation Mycobacteria Mycobacteriophage Recombineering bacteriophage DNA genomic DNA oligonucleotide Rac protein recombinant protein RecT protein bacteriophage recombineering of electroporated DNA bacterium culture electroporation genetic engineering genetic manipulation genetic recombination methodology mutant mycobacteriophage Mycobacterium smegmatis natural science nonhuman polymerase chain reaction prophage recombineering |
| spellingShingle |
BRED Electroporation Mycobacteria Mycobacteriophage Recombineering bacteriophage DNA genomic DNA oligonucleotide Rac protein recombinant protein RecT protein bacteriophage recombineering of electroporated DNA bacterium culture electroporation genetic engineering genetic manipulation genetic recombination methodology mutant mycobacteriophage Mycobacterium smegmatis natural science nonhuman polymerase chain reaction prophage recombineering Marinelli, L.J. Piuri, M. Hatfull, G.F. Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA |
| topic_facet |
BRED Electroporation Mycobacteria Mycobacteriophage Recombineering bacteriophage DNA genomic DNA oligonucleotide Rac protein recombinant protein RecT protein bacteriophage recombineering of electroporated DNA bacterium culture electroporation genetic engineering genetic manipulation genetic recombination methodology mutant mycobacteriophage Mycobacterium smegmatis natural science nonhuman polymerase chain reaction prophage recombineering |
| description |
We describe a recombineering-based method for the genetic manipulation of lytically replicating bacteriophages, focusing on mycobacteriophages. The approach utilizes recombineering-proficient strains of Mycobacterium smegmatis and employs a cotransformation strategy with purified phage genomic DNA and a mutagenic substrate, which selects for only those cells that are competent to take up DNA. The cotransformation method, combined with the high rates of recombination obtained in M. smegmatis recombineering strains, allows for the efficient and rapid generation of bacteriophage mutants. © 2019, Springer Science+Business Media, LLC, part of Springer Nature. |
| format |
SER |
| author |
Marinelli, L.J. Piuri, M. Hatfull, G.F. |
| author_facet |
Marinelli, L.J. Piuri, M. Hatfull, G.F. |
| author_sort |
Marinelli, L.J. |
| title |
Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA |
| title_short |
Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA |
| title_full |
Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA |
| title_fullStr |
Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA |
| title_full_unstemmed |
Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA |
| title_sort |
genetic manipulation of lytic bacteriophages with bred: bacteriophage recombineering of electroporated dna |
| url |
http://hdl.handle.net/20.500.12110/paper_10643745_v1898_n_p69_Marinelli |
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