Modulation of Maternal LIF Producers T Cells by Trophoblast and Paternal Antigens

Problem Fetal implantation enhances the production of essential growth factors such as LIF (leukaemia inhibitory factor), hence we investigated the contribution of maternal CD4 cells, activated by paternal or trophoblast antigens and its modulation by VIP (vasoactive intestinal peptide) and progeste...

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Detalles Bibliográficos
Autores principales: Fraccaroli, L., Grasso, E., Zeitler, E., Lombardi, E., Gogorza, S., Etchepareborda, J.J., Nagle, C., Cortelezzi, M., Leirós, C.P., Ramhorst, R.
Formato: JOUR
Materias:
Chemokines
Recurrent spontaneous abortions
Tolerance and pregnancy
antigen
gelatinase B
leukemia inhibitory factor
progesterone
STAT3 protein
vasoactive intestinal polypeptide
article
CD4+ T lymphocyte
cell stimulation
controlled study
enzyme activity
female
female fertility
fetoplacental unit
human
human cell
inflammation
lymphocytic infiltration
nidation
peripheral blood mononuclear cell
priority journal
protein synthesis
recurrent abortion
spontaneous abortion
T lymphocyte
trophoblast
Abortion, Habitual
Antigens
CD4-Positive T-Lymphocytes
Cells, Cultured
Coculture Techniques
Embryo Implantation
Female
Humans
Immune Tolerance
Leukemia Inhibitory Factor
Leukocytes, Mononuclear
Male
Pregnancy
Progesterone
Trophoblasts
Vasoactive Intestinal Peptide
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10467408_v65_n2_p133_Fraccaroli
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Problem Fetal implantation enhances the production of essential growth factors such as LIF (leukaemia inhibitory factor), hence we investigated the contribution of maternal CD4 cells, activated by paternal or trophoblast antigens and its modulation by VIP (vasoactive intestinal peptide) and progesterone.Method of study We performed co cultures of trophoblast cells (Swan 71 cell line) or paternal antigens and PBMCs from patients with recurrent spontaneous abortions (RSA) and fertile women.Result Fertile-CD4+LIF+ cells were increased by VIP and progesterone in response to paternal and trophoblast antigens. Also MMP 9 activity was decreased and pSTAT3/STAT3 ratio was increased. RSA patients have decreased levels of LIF expression which could not be modulated by VIP and progesterone and displayed a reduced number of endometrial infiltrated CD4+LIF+ cells compared with fertile women.Conclusion The decrease of CD4+LIF+ cells in RSA patients could be related with the exacerbated inflammatory response observed in the maternal fetal dialogue model. © 2010 John Wiley & Sons A/S.

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